ID A0A366DQX0_9NOCA Unreviewed; 663 AA.
AC A0A366DQX0;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=DFR74_103125 {ECO:0000313|EMBL:RBO92482.1};
OS Nocardia puris.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae;
OC Nocardia.
OX NCBI_TaxID=208602 {ECO:0000313|EMBL:RBO92482.1, ECO:0000313|Proteomes:UP000252586};
RN [1] {ECO:0000313|EMBL:RBO92482.1, ECO:0000313|Proteomes:UP000252586}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44599 {ECO:0000313|EMBL:RBO92482.1,
RC ECO:0000313|Proteomes:UP000252586};
RA Goeker M.;
RT "Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing the
RT most valuable type-strain genomes for metagenomic binning, comparative
RT biology and taxonomic classification.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RBO92482.1}.
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DR EMBL; QNRE01000003; RBO92482.1; -; Genomic_DNA.
DR RefSeq; WP_067510139.1; NZ_QNRE01000003.1.
DR AlphaFoldDB; A0A366DQX0; -.
DR STRING; 1210090.GCA_001613185_03614; -.
DR OrthoDB; 9784823at2; -.
DR Proteomes; UP000252586; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000252586};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 9..141
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 153..453
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 663 AA; 74327 MW; CD517A78E007764C CRC64;
MNTNKHTELA NHAWSVADLL RGDYKQSDYG KVILPFTVLR RLECVLEPTR EKVSAVVEAR
KDSDLDLDPF LRKASGTSFY NVTPLTLKSI LADSGHAAKN MINYIGGFSE NAREVLEKYE
FAQQVKRLDS ANLLYQVIGR FADLDLRPDV VSNHNMGYVF EELIRRFAEQ SNETAGEHFT
PREVIELMVN LLIAPDGELV GTPGVTRKVL DPACGTGGML SAAEEHILAM NPQARVEVYG
QELNPESWAI CRSDLMIKGQ VPDNIAFGNS FSDDGHKHLR ADYLLANPPF GVEWKKVKED
VEWEHKNLGD AGRFGAGLPR INDGSLLFLQ HMISKMKPVG VDGGGSRVAI VFNGSPLFTG
AAESGESNIR KWIIENDWLE AIVALPDQLF YNTGISTYFW ILTNRKSPDH RGKVVLLDAR
DYWQKMRKSL GDKRKYVGKE QIDEITLLYR DALDVAADPD HPLHGKVKVF ANNDFGYRRI
TVERPLRLRF ELTEESLDQL VSAAAVRKAY TDPGVFRGLL APLVGSVWKT KADTVNALKD
AVVGAGEPWP TGAPFAKALR DALGVRDPEG ELQLLKGAPE PDPDLRDYEN VPLDEDVDEY
LAREVLPHVP DAWIDHSKTK IGYEIPFTRH FYVYTPPRPL EEIDAELREL ETEIQKLLGE
VTQ
//
