ID   A0A366I3H6_9GAMM        Unreviewed;       915 AA.
AC   A0A366I3H6;
DT   07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT   07-NOV-2018, sequence version 1.
DT   24-JAN-2024, entry version 19.
DE   SubName: Full=Type IV secretion system protein VirB4 {ECO:0000313|EMBL:RBP60988.1};
GN   ORFNames=DES54_12840 {ECO:0000313|EMBL:RBP60988.1};
OS   Brenneria salicis ATCC 15712 = DSM 30166.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Brenneria.
OX   NCBI_TaxID=714314 {ECO:0000313|EMBL:RBP60988.1, ECO:0000313|Proteomes:UP000253046};
RN   [1] {ECO:0000313|EMBL:RBP60988.1, ECO:0000313|Proteomes:UP000253046}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 30166 {ECO:0000313|EMBL:RBP60988.1,
RC   ECO:0000313|Proteomes:UP000253046};
RA   Goeker M.;
RT   "Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing the
RT   most valuable type-strain genomes for metagenomic binning, comparative
RT   biology and taxonomic classification.";
RL   Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the TrbE/VirB4 family.
CC       {ECO:0000256|ARBA:ARBA00006512}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RBP60988.1}.
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DR   EMBL; QNRY01000028; RBP60988.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A366I3H6; -.
DR   OrthoDB; 9816422at2; -.
DR   Proteomes; UP000253046; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   CDD; cd01127; TrwB_TraG_TraD_VirD4; 1.
DR   Gene3D; 1.10.8.730; -; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR018145; CagE_TrbE_VirB_cntrl_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR043964; P-loop_TraG.
DR   InterPro; IPR007792; T4SS_VirB3/TrbD/AvhB.
DR   PANTHER; PTHR30121:SF6; TYPE IV SECRETION SYSTEM PROTEIN CAGE; 1.
DR   PANTHER; PTHR30121; UNCHARACTERIZED PROTEIN YJGR-RELATED; 1.
DR   Pfam; PF03135; CagE_TrbE_VirB; 1.
DR   Pfam; PF19044; P-loop_TraG; 1.
DR   Pfam; PF05101; VirB3; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Coiled coil {ECO:0000256|SAM:Coils}; Membrane {ECO:0000256|SAM:Phobius};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        12..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          271..472
FT                   /note="CagE TrbE VirB component of type IV transporter
FT                   system central"
FT                   /evidence="ECO:0000259|Pfam:PF03135"
FT   DOMAIN          535..820
FT                   /note="TraG P-loop"
FT                   /evidence="ECO:0000259|Pfam:PF19044"
FT   COILED          371..398
FT                   /evidence="ECO:0000256|SAM:Coils"
SQ   SEQUENCE   915 AA;  104138 MW;  B9A140605A72A689 CRC64;
     MTTLNKALTR PAAIMGIPLV PFVIVSGTLV LLSVYLSYYL ALLLIPAWIE MKSKARTDIH
     YFGLLWLAFK TRGRFATNRH YGANAMLANR YDAVDVSEFT KKMKLNERIT LDKYIPYSSH
     IHPQVIRNRH GDLVATWELA GVVFECEDEH HLTFLATHLN NVIRSYEGLP VTFYIHRIRE
     QYRDAFEAHS GIPFADEVSA RYYRPIKDKP FWRHRLFFTV CYAPFSRLEK KAMKRQPVGK
     RHAALDDALK AMLEHQEALT SALSRYMATP LGVYEENGRV YSSQLAFYHR LLTGKWQKVA
     VTHSPFYDTL STPDVFFSTD TAECQTVDGS RFFRSLEIKD YSPQTHTGLL DALLYAKSEY
     VLTQSFTCMA RDEAQNHIRL AEKRLNSADD DAISQREELI VLRDLLQSGH ISCGKYHFSL
     LVSSNSADRV VKDTNALAQP FADLGIMTSL STLSLPAAYL AQLPGVYTLR PRLVAVSSQN
     FADMASLHNF HPHKRDGNPW GEAIAILTSP GGGGYYLNLH DSQAGRDDFN EKTPGNTAII
     GKTGSGKTLL MTMMQQLMQK YRNPATFAVS ATIKRLTTVY FDKDRAAEMS IRQMGGRYFR
     IRTGVPSGFN PFSLAPSRRN ISFIKRLVRM LCRRNGKPLD PRDEERISTA VDTIMLDYPP
     EYRRYGITRL LEVLPEPPTK DARTNGLRIR LKQWAQGGEF GWVFDNEEDT FTISDIDNFG
     IDGTEFLDDD DIRGPITFYL LYRVTSLLDG RRLVMFMDEF WKWLADVEFS RFSLNMLKVI
     RKLNGIFIPA TQSPDEIVKH PIAPAIIEQC GTQIFLANPK ASQADYVEKM KVPESVYDIV
     RNLDPGEHYM VVLKTPLRAG ETRPFVSMAK MDLSGLGKIT KLLSGSEDNL KIFDALYQDG
     MKHEEWKASF LKQAI
//