ID A0A367EBX4_9ACTN Unreviewed; 610 AA.
AC A0A367EBX4;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 24-JAN-2024, entry version 14.
DE RecName: Full=Beta-galactosidase {ECO:0000256|RuleBase:RU000675};
DE EC=3.2.1.23 {ECO:0000256|RuleBase:RU000675};
GN ORFNames=DTL70_29630 {ECO:0000313|EMBL:RCG15531.1};
OS Streptomyces diacarni.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=2800381 {ECO:0000313|EMBL:RCG15531.1, ECO:0000313|Proteomes:UP000252914};
RN [1] {ECO:0000313|EMBL:RCG15531.1, ECO:0000313|Proteomes:UP000252914}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LHW51701 {ECO:0000313|EMBL:RCG15531.1,
RC ECO:0000313|Proteomes:UP000252914};
RA Li L.;
RT "Streptomyces reniochalinae sp. nov. and Streptomyces diacarnus sp. nov.
RT from marine sponges.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|RuleBase:RU000675};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RCG15531.1}.
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DR EMBL; QOIN01000066; RCG15531.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A367EBX4; -.
DR OrthoDB; 9813184at2; -.
DR Proteomes; UP000252914; Unassembled WGS sequence.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR026283; B-gal_1-like.
DR InterPro; IPR048912; BetaGal1-like_ABD1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421:SF165; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21317; BetaGal_ABD_1; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PIRSF; PIRSF006336; B-gal; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW Reference proteome {ECO:0000313|Proteomes:UP000252914}.
FT DOMAIN 27..339
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 388..496
FT /note="Beta-galactosidase 1-like first all-beta"
FT /evidence="ECO:0000259|Pfam:PF21317"
FT DOMAIN 522..577
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
FT ACT_SITE 174
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
FT ACT_SITE 250
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
SQ SEQUENCE 610 AA; 65992 MW; FF2ADDE9DBDB582D CRC64;
MTPPTTGPAA SSAGRAAATL THADGRFLRG GRPHRVLSGS LHYFRVHPDH WADRLDRLAA
LGLNTVDTYV PWNFHERHPE DIRFDGWRDL PRFVQLAQRA GLDVIVRPGP YICAEWDNGG
LPAWLTGTPG MRPRTSHPPF LDAVGRWFDA LIPKVAALQA ARGGPVVAVQ IENEYGSYGD
DHRYLRWVRD ALVDRGITEL LYTADGPTPL MLDGGTLPGE LTAATFGSRP ADAAALLHTR
RQGEPFFCAE FWNGWFDHWG EKHHVRSADS AAGTVRELLD ADGSISLYMA HGGSNFGLWA
GANHDGETFQ PTVTSYDSDA PIAEHGALTP KFHALRATLA TALGAAAPQR PLPADPPLLA
PRTLPVSRQA ALLEALRATT DPVHAPSPLS FEELGQSAGL VLYTAHPLVP PGRQEVTVTG
LHDRAQIFLD GVPAAVLDRE TASFTVEGTG ERTRLELLVE NQGRINYGPL LGQGKGILGG
VRVERRLVHG WSMHRLPLDA WTSQETAHAA SAAAPGGTAG FAHAHLELVE PADTFLALPG
FAKGFVWVNG TLLGRYWEVG PQRTLYVPGP LLRAGANTLT VLELERFGGH LALVDRPELG
PAEQYVETFE
//
