ID   A0A369AST0_9ENTE        Unreviewed;       304 AA.
AC   A0A369AST0;
DT   07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT   07-NOV-2018, sequence version 1.
DT   27-MAR-2024, entry version 17.
DE   SubName: Full=N-acetylneuraminate lyase {ECO:0000313|EMBL:RSU00897.1};
GN   ORFNames=CBF32_09815 {ECO:0000313|EMBL:RSU00897.1};
OS   Vagococcus fluvialis.
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae; Vagococcus.
OX   NCBI_TaxID=2738 {ECO:0000313|EMBL:RSU00897.1, ECO:0000313|Proteomes:UP000288197};
RN   [1] {ECO:0000313|EMBL:RSU00897.1, ECO:0000313|Proteomes:UP000288197}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCFB 2497 {ECO:0000313|EMBL:RSU00897.1,
RC   ECO:0000313|Proteomes:UP000288197};
RA   Gulvik C.A.;
RT   "Vagococcus spp. assemblies.";
RL   Submitted (MAY-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the DapA family.
CC       {ECO:0000256|PIRNR:PIRNR001365}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RSU00897.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; NGJX01000010; RSU00897.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A369AST0; -.
DR   OrthoDB; 9782828at2; -.
DR   Proteomes; UP000288197; Unassembled WGS sequence.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR002220; DapA-like.
DR   PANTHER; PTHR42849; N-ACETYLNEURAMINATE LYASE; 1.
DR   PANTHER; PTHR42849:SF1; N-ACETYLNEURAMINATE LYASE; 1.
DR   Pfam; PF00701; DHDPS; 1.
DR   PIRSF; PIRSF001365; DHDPS; 1.
DR   PRINTS; PR00146; DHPICSNTHASE.
DR   SMART; SM01130; DHDPS; 1.
DR   SUPFAM; SSF51569; Aldolase; 1.
PE   3: Inferred from homology;
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|PIRNR:PIRNR001365};
KW   Reference proteome {ECO:0000313|Proteomes:UP000288197}.
FT   ACT_SITE        138
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001365-1"
FT   ACT_SITE        166
FT                   /note="Schiff-base intermediate with substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001365-1"
FT   BINDING         208
FT                   /ligand="pyruvate"
FT                   /ligand_id="ChEBI:CHEBI:15361"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001365-2"
SQ   SEQUENCE   304 AA;  33422 MW;  24BD9A3A5F0BC794 CRC64;
     MTKLEKYKGI IPAFYACYDE NGNISGERVQ ELAKFYLDKG VKGLYVGGSS GECIYQNVTE
     RKEVLENVMA AVGGKMTIIA HVAAPSTRDS VELAKHAESL GVDALAAIPP IYFGLPEFAI
     RDYWTAMMNA SSLDFIIYNI PQTTGYALSV NMLKEMMSFD QVIGVKNSSM PVQDIYLFKR
     NAGKEIVVFN GPDEQFVAGR IMGADAGIGG TYGVMPELFL AANQAIEDSE IEKAQEIQIM
     IDDIIFKMLE AKGNLYDVMK AVLVKRGITI GSVRAPLPRI TSEDMSIVEE VYGMIELAIK
     KYSL
//