ID A0A369QSV4_9BACT Unreviewed; 506 AA.
AC A0A369QSV4;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 24-JAN-2024, entry version 15.
DE RecName: Full=Homoserine O-acetyltransferase {ECO:0000256|HAMAP-Rule:MF_00296};
DE Short=HAT {ECO:0000256|HAMAP-Rule:MF_00296};
DE EC=2.3.1.31 {ECO:0000256|HAMAP-Rule:MF_00296};
DE AltName: Full=Homoserine transacetylase {ECO:0000256|HAMAP-Rule:MF_00296};
DE Short=HTA {ECO:0000256|HAMAP-Rule:MF_00296};
GN Name=metX {ECO:0000313|EMBL:RDC65248.1};
GN Synonyms=metXA {ECO:0000256|HAMAP-Rule:MF_00296};
GN ORFNames=AHMF7616_03878 {ECO:0000313|EMBL:RDC65248.1};
OS Adhaeribacter pallidiroseus.
OC Bacteria; Bacteroidota; Cytophagia; Cytophagales; Hymenobacteraceae;
OC Adhaeribacter.
OX NCBI_TaxID=2072847 {ECO:0000313|EMBL:RDC65248.1, ECO:0000313|Proteomes:UP000253919};
RN [1] {ECO:0000313|EMBL:RDC65248.1, ECO:0000313|Proteomes:UP000253919}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HMF7616 {ECO:0000313|EMBL:RDC65248.1,
RC ECO:0000313|Proteomes:UP000253919};
RA Kang H., Kang J., Cha I., Kim H., Joh K.;
RT "Adhaeribacter sp. HMF7616 genome sequencing and assembly.";
RL Submitted (APR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transfers an acetyl group from acetyl-CoA to L-homoserine,
CC forming acetyl-L-homoserine. {ECO:0000256|HAMAP-Rule:MF_00296}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine;
CC Xref=Rhea:RHEA:13701, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC ChEBI:CHEBI:57476, ChEBI:CHEBI:57716; EC=2.3.1.31;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00296};
CC -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo
CC pathway; O-acetyl-L-homoserine from L-homoserine: step 1/1.
CC {ECO:0000256|HAMAP-Rule:MF_00296}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00296}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00296}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. MetX family.
CC {ECO:0000256|HAMAP-Rule:MF_00296}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_00296}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RDC65248.1}.
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DR EMBL; QASA01000001; RDC65248.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A369QSV4; -.
DR OrthoDB; 9800754at2; -.
DR UniPathway; UPA00051; UER00074.
DR Proteomes; UP000253919; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004414; F:homoserine O-acetyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR HAMAP; MF_00296; MetX_acyltransf; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR008220; HAT_MetX-like.
DR NCBIfam; TIGR01392; homoserO_Ac_trn; 1.
DR PANTHER; PTHR32268; HOMOSERINE O-ACETYLTRANSFERASE; 1.
DR PANTHER; PTHR32268:SF11; HOMOSERINE O-ACETYLTRANSFERASE; 1.
DR Pfam; PF00561; Abhydrolase_1; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|HAMAP-Rule:MF_00296,
KW ECO:0000313|EMBL:RDC65248.1};
KW Amino-acid biosynthesis {ECO:0000256|HAMAP-Rule:MF_00296};
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00296};
KW Methionine biosynthesis {ECO:0000256|HAMAP-Rule:MF_00296};
KW Reference proteome {ECO:0000313|Proteomes:UP000253919};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_00296}.
FT DOMAIN 40..322
FT /note="AB hydrolase-1"
FT /evidence="ECO:0000259|Pfam:PF00561"
FT ACT_SITE 135
FT /note="Nucleophile"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00296"
FT ACT_SITE 289
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00296"
FT ACT_SITE 318
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00296"
FT BINDING 201
FT /ligand="substrate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00296"
FT BINDING 319
FT /ligand="substrate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00296"
SQ SEQUENCE 506 AA; 57475 MW; E571A019EDFA8EC7 CRC64;
MSQEILHYPQ PFLLECGKTL PQLQITYHTY GTYNPEKNNV IWVCHALTAN ADVFDWWKGL
FGSGCLFNPD DYFIVCANIL GSCYGTTGPL AENIATGAAY FQLFPDISIR DMVAAHEILR
QHLGIFKIYS LIGGSLGGQQ ALEWSIQYPD LIENLLIVAT NASHSPWGIA FNEAQRMAIR
ADETYNLNVP EGGRKGLKAA RAVALLSYRT YHTYGRTQCE TDLNKTDNFK ASSYQNYQGD
KLVNRFNAYS YVSLSKTMDS HNVGRNQPSI EQALQQIKAK TLVIGISSDL LFPPQEQQYL
TEHIPGAVYH EIDSFYGHDG FLIETEKLTQ LIETFLAVPI TPQLKLDKMI SQIVKEEFYF
RGAFMSLFID IEFLLGDTIA TILTGDNELK LNLIEFVNPK LMLEPKLSLL HKILNQKYFD
LYSKKYKDGI NELRKFTDLR NSFAHKRISI DNGNKKLNFI VMDNGKLIDN SFTFENLETK
FTELKALLTL VNTLHDEIVK SQTDKA
//
