ID A0A369TNR3_9RHOB Unreviewed; 639 AA.
AC A0A369TNR3;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=3-hydroxyisobutyrate dehydrogenase {ECO:0000256|RuleBase:RU910714};
DE Short=HIBADH {ECO:0000256|RuleBase:RU910714};
DE EC=1.1.1.31 {ECO:0000256|RuleBase:RU910714};
GN Name=mmsB {ECO:0000313|EMBL:RDD66931.1};
GN ORFNames=DU478_08280 {ECO:0000313|EMBL:RDD66931.1};
OS Thalassococcus profundi.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Roseobacteraceae; Thalassococcus.
OX NCBI_TaxID=2282382 {ECO:0000313|EMBL:RDD66931.1, ECO:0000313|Proteomes:UP000253977};
RN [1] {ECO:0000313|EMBL:RDD66931.1, ECO:0000313|Proteomes:UP000253977}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WRAS1 {ECO:0000313|EMBL:RDD66931.1,
RC ECO:0000313|Proteomes:UP000253977};
RA Yu M.;
RT "Thalassococcus profundi sp. nov., a marine bacterium isolated from deep
RT seawater of Okinawa Trough.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-
CC oxopropanoate + H(+) + NADH; Xref=Rhea:RHEA:17681, ChEBI:CHEBI:11805,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57700,
CC ChEBI:CHEBI:57945; EC=1.1.1.31;
CC Evidence={ECO:0000256|RuleBase:RU910714};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) +
CC NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;
CC Evidence={ECO:0000256|ARBA:ARBA00023693};
CC -!- PATHWAY: Amino-acid degradation; L-valine degradation.
CC {ECO:0000256|RuleBase:RU910714}.
CC -!- SIMILARITY: Belongs to the HIBADH-related family.
CC {ECO:0000256|ARBA:ARBA00009080, ECO:0000256|RuleBase:RU910714}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RDD66931.1}.
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DR EMBL; QPMK01000004; RDD66931.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A369TNR3; -.
DR OrthoDB; 9790967at2; -.
DR UniPathway; UPA00362; -.
DR Proteomes; UP000253977; Unassembled WGS sequence.
DR GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:RHEA.
DR GO; GO:0008442; F:3-hydroxyisobutyrate dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR GO; GO:0006574; P:valine catabolic process; IEA:UniProtKB-UniPathway.
DR CDD; cd06558; crotonase-like; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR002204; 3-OH-isobutyrate_DH-rel_CS.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR013328; 6PGD_dom2.
DR InterPro; IPR006115; 6PGDH_NADP-bd.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR045004; ECH_dom.
DR InterPro; IPR011548; HIBADH.
DR InterPro; IPR029154; HIBADH-like_NADP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR NCBIfam; TIGR01692; HIBADH; 1.
DR PANTHER; PTHR22981:SF7; 3-HYDROXYISOBUTYRATE DEHYDROGENASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR22981; 3-HYDROXYISOBUTYRATE DEHYDROGENASE-RELATED; 1.
DR Pfam; PF16113; ECH_2; 1.
DR Pfam; PF14833; NAD_binding_11; 1.
DR Pfam; PF03446; NAD_binding_2; 1.
DR SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 1.
DR SUPFAM; SSF52096; ClpP/crotonase; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS00895; 3_HYDROXYISOBUT_DH; 1.
PE 3: Inferred from homology;
KW Branched-chain amino acid catabolism {ECO:0000256|ARBA:ARBA00022456,
KW ECO:0000256|RuleBase:RU910714};
KW NAD {ECO:0000256|ARBA:ARBA00023027, ECO:0000256|RuleBase:RU910714};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU910714};
KW Reference proteome {ECO:0000313|Proteomes:UP000253977}.
FT DOMAIN 13..332
FT /note="Enoyl-CoA hydratase/isomerase"
FT /evidence="ECO:0000259|Pfam:PF16113"
FT DOMAIN 351..502
FT /note="6-phosphogluconate dehydrogenase NADP-binding"
FT /evidence="ECO:0000259|Pfam:PF03446"
FT DOMAIN 505..630
FT /note="3-hydroxyisobutyrate dehydrogenase-like NAD-binding"
FT /evidence="ECO:0000259|Pfam:PF14833"
SQ SEQUENCE 639 AA; 67551 MW; 774614EBFF3459A3 CRC64;
MTDFHIRTEG RSGRITLTRP KALNALSYAM CRAIEDALLT WRRDPEINLV LIDADGDKAF
CAGGDIQELY DTGTRGDLAY GQRFWRDEYR MNALIAEYPK PVVSFLQGFT MGGGVGLGCQ
GSHRIVSDSS RIAMPECGIG LVPDVGGSLL LARAPGRLGE YIGITGSRLG PGDALLAGFA
DYFIPEADWP ALKDTLVRDG SADAIAEAAQ TAPEAPLAAL RDKIDRHFAG ETLRDVIASL
RAGTGDFATK TLETLSRHAP LSMACTLEIL HRLRQGAPDI RKALELEYRF THRAMADGDL
LEGIRAAIVD KDRSPRWQHT LDGVPATAMS RMLMPVREAK LQFHEEERAM KIGFIGLGNM
GAPMAANLAK AGHSVRGFDT ASVVVDGVEP VNSATEAAEG AEVVITMLPN GDILRKVAAE
VIPAMEKGAV LLDCSTVDVD SARDVAAEAE AAGLLALDAP VSGGIGGASA GTLTFMVGGA
PEGFAKAKPL FDIMGQKAVH CGASGNGQAA KICNNMILGV TMIATCEAFA LADKLGLDRQ
AMFDVVSTSS GYSWSMNAYC PAPGIGPHSP ADNGYTPGFA AELMLKDLGL AQQAAEGADA
DTPMGAAALA LYRQFVEDED GRGRDFSAML LRFESRGRG
//
