ID A0A370AQQ4_9SPIO Unreviewed; 359 AA.
AC A0A370AQQ4;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 24-JAN-2024, entry version 14.
DE RecName: Full=dTDP-glucose 4,6-dehydratase {ECO:0000256|ARBA:ARBA00011990, ECO:0000256|RuleBase:RU004473};
DE EC=4.2.1.46 {ECO:0000256|ARBA:ARBA00011990, ECO:0000256|RuleBase:RU004473};
GN Name=rfbB {ECO:0000313|EMBL:RDG29806.1};
GN ORFNames=DV872_19685 {ECO:0000313|EMBL:RDG29806.1};
OS Oceanispirochaeta sp. M1.
OC Bacteria; Spirochaetota; Spirochaetia; Spirochaetales; Spirochaetaceae;
OC Oceanispirochaeta.
OX NCBI_TaxID=2283433 {ECO:0000313|EMBL:RDG29806.1, ECO:0000313|Proteomes:UP000253869};
RN [1] {ECO:0000313|EMBL:RDG29806.1, ECO:0000313|Proteomes:UP000253869}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M1 {ECO:0000313|EMBL:RDG29806.1,
RC ECO:0000313|Proteomes:UP000253869};
RA Yadav S., Villanueva L., Damste J.S.S.;
RT "Draft genome sequence of Oceanispirochaeta sp. strain M1.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose
CC + H2O; Xref=Rhea:RHEA:17221, ChEBI:CHEBI:15377, ChEBI:CHEBI:57477,
CC ChEBI:CHEBI:57649; EC=4.2.1.46;
CC Evidence={ECO:0000256|ARBA:ARBA00001539,
CC ECO:0000256|RuleBase:RU004473};
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC Evidence={ECO:0000256|ARBA:ARBA00001911,
CC ECO:0000256|RuleBase:RU004473};
CC -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC family. dTDP-glucose dehydratase subfamily.
CC {ECO:0000256|ARBA:ARBA00008178, ECO:0000256|RuleBase:RU004473}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RDG29806.1}.
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DR EMBL; QQPQ01000034; RDG29806.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A370AQQ4; -.
DR OrthoDB; 9811743at2; -.
DR Proteomes; UP000253869; Unassembled WGS sequence.
DR GO; GO:0008460; F:dTDP-glucose 4,6-dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0009225; P:nucleotide-sugar metabolic process; IEA:InterPro.
DR CDD; cd05246; dTDP_GD_SDR_e; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.90.25.10; UDP-galactose 4-epimerase, domain 1; 1.
DR InterPro; IPR005888; dTDP_Gluc_deHydtase.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR NCBIfam; TIGR01181; dTDP_gluc_dehyt; 1.
DR PANTHER; PTHR43000:SF7; DTDP-D-GLUCOSE 4,6-DEHYDRATASE; 1.
DR PANTHER; PTHR43000; DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED; 1.
DR Pfam; PF16363; GDP_Man_Dehyd; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|RuleBase:RU004473, ECO:0000313|EMBL:RDG29806.1};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Reference proteome {ECO:0000313|Proteomes:UP000253869}.
FT DOMAIN 11..329
FT /note="NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF16363"
SQ SEQUENCE 359 AA; 40585 MW; 62FF7B75D30EF56D CRC64;
MTSSRSFKNI LVTGGAGFIG SNLIRVLLTK SDFTGRIINL DKLTYAGNAS SLDDVASQFP
SRYVFKQGDI CDREFVDSLF KEYDIDSVCH LAAESHVDRS IEGPEAFITT NVNGTFTLLD
VARKAWDGSY DGKLFHHVST DEVYGSLGDT GYFFEDTPYD PRSPYSASKA SSDHLVMAYY
HTYGMPITMT NCSNNYGPYQ FPEKLIPVMI ENMLQGKALP VYGDGKNIRD WLYVEDHNSA
VWAVMQGGRL GETYNIGGEN EWENIKLVHT LCDVVADEAG LDASSLKGLI SYVKDRPGHD
QRYAINCDKL KNELGWKQSL DFEEGLQKTV RWYLDNRKWI ENITSGEYLK WMEKNYKGR
//
