ID A0A370BBS4_9ACTN Unreviewed; 683 AA.
AC A0A370BBS4;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 24-JAN-2024, entry version 15.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
DE Short=Beta-gal {ECO:0000256|PIRNR:PIRNR001084};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
GN ORFNames=DVH02_05410 {ECO:0000313|EMBL:RDG39091.1};
OS Streptomyces corynorhini.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=2282652 {ECO:0000313|EMBL:RDG39091.1, ECO:0000313|Proteomes:UP000253741};
RN [1] {ECO:0000313|EMBL:RDG39091.1, ECO:0000313|Proteomes:UP000253741}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AC230 {ECO:0000313|EMBL:RDG39091.1,
RC ECO:0000313|Proteomes:UP000253741};
RA Dunlap C.;
RT "Streptomyces species from bats.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|PIRNR:PIRNR001084};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 42 family.
CC {ECO:0000256|ARBA:ARBA00005940, ECO:0000256|PIRNR:PIRNR001084}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RDG39091.1}.
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DR EMBL; QQNA01000031; RDG39091.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A370BBS4; -.
DR OrthoDB; 9800974at2; -.
DR Proteomes; UP000253741; Unassembled WGS sequence.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006012; P:galactose metabolic process; IEA:InterPro.
DR CDD; cd03143; A4_beta-galactosidase_middle_domain; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR013739; Beta_galactosidase_C.
DR InterPro; IPR013738; Beta_galactosidase_Trimer.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR003476; Glyco_hydro_42.
DR InterPro; IPR013529; Glyco_hydro_42_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR36447; BETA-GALACTOSIDASE GANA; 1.
DR PANTHER; PTHR36447:SF1; BETA-GALACTOSIDASE GANA; 1.
DR Pfam; PF02449; Glyco_hydro_42; 1.
DR Pfam; PF08533; Glyco_hydro_42C; 1.
DR Pfam; PF08532; Glyco_hydro_42M; 1.
DR PIRSF; PIRSF001084; B-galactosidase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR001084};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR001084};
KW Reference proteome {ECO:0000313|Proteomes:UP000253741}.
FT DOMAIN 26..394
FT /note="Glycoside hydrolase family 42 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02449"
FT DOMAIN 408..611
FT /note="Beta-galactosidase trimerisation"
FT /evidence="ECO:0000259|Pfam:PF08532"
FT DOMAIN 623..683
FT /note="Beta-galactosidase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08533"
FT ACT_SITE 162
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT ACT_SITE 319
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT BINDING 123
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 161
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 327
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
SQ SEQUENCE 683 AA; 75496 MW; 88772A6EC3146C29 CRC64;
MTRVPSPRWL RRPHDDRTPR FAYGADYNPE QWPREVWPED IRLMREAGVN IVSLAIFSWA
RIQPAPDTWE FGWLDEIMDL LHEGGIAVDL ATATASPPPW LTTLHPEILP VTAAGETVWP
GARQHWRPTS PVFRSYALKL ARKMAERYGD HPALTAWHIS NELGCHNIYD YSDDAAHAFR
GWLTDRYGSI EALNHAWGTA FWAQRYTEWD QILPPRLAAS HPNPTQQLDF KRFSSDALKD
YLRAERDLLY RLTPDTPVTT NFMVMGETKG MHYADWAREV DFVSNDHYTL PGPQRLDELS
FSANLTGSIA GGHPWFLMEH STSAVNWQPV NVAKRPDELA RDSLLHVAHG ADAVCFFQWR
QSAAGAEKYH SAMVPHAGPD SEVFRSVAAL GALLGELSEV VGSHGRPARA AVLFDWESWW
ASEQDSHPTS LLRYRQEALD WYSAFLDLGV RADVVHRGAS LDGYDLVVAP VLHVVPRDLA
KELTGYVEGG GHLVTTYFSG VVDENDHAWL GGYPGALREL LGIRIEEFGP LLDGQHVTLD
DGSTGTLWTD RITVTDDAVE VLASYRTGDQ SGRPAITRRA VGGGSAAYVS TRLGAEGLRG
VLAPLLARAG VAGELPEAAR GRVELVVRGD GDTAYWFLVN RTDEPVELAR LSGTLLAGTA
GSTRHGAEGP LVLGPRGVAV VRR
//
