ID A0A370ENK3_9FLAO Unreviewed; 855 AA.
AC A0A370ENK3;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=DNA ligase (ATP) {ECO:0000256|ARBA:ARBA00012727};
DE EC=6.5.1.1 {ECO:0000256|ARBA:ARBA00012727};
DE AltName: Full=NHEJ DNA polymerase {ECO:0000256|ARBA:ARBA00029943};
GN ORFNames=DEU42_107102 {ECO:0000313|EMBL:RDI10443.1};
OS Flavobacterium sp. AG291.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=2184000 {ECO:0000313|EMBL:RDI10443.1, ECO:0000313|Proteomes:UP000254305};
RN [1] {ECO:0000313|EMBL:RDI10443.1, ECO:0000313|Proteomes:UP000254305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AG291 {ECO:0000313|EMBL:RDI10443.1,
RC ECO:0000313|Proteomes:UP000254305};
RA Venturi V.;
RT "Genome sequencing of rice bacterial endophytes.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP +
CC diphosphate.; EC=6.5.1.1; Evidence={ECO:0000256|ARBA:ARBA00034003};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RDI10443.1}.
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DR EMBL; QQAR01000007; RDI10443.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A370ENK3; -.
DR OrthoDB; 9802472at2; -.
DR Proteomes; UP000254305; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:UniProtKB-EC.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR CDD; cd07906; Adenylation_DNA_ligase_LigD_LigC; 1.
DR CDD; cd04865; LigD_Pol_like_2; 1.
DR CDD; cd07971; OBF_DNA_ligase_LigD; 1.
DR Gene3D; 3.30.1490.70; -; 1.
DR Gene3D; 3.30.470.30; DNA ligase/mRNA capping enzyme; 1.
DR Gene3D; 3.90.920.10; DNA primase, PRIM domain; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR InterPro; IPR012309; DNA_ligase_ATP-dep_C.
DR InterPro; IPR012310; DNA_ligase_ATP-dep_cent.
DR InterPro; IPR014146; LigD_ligase_dom.
DR InterPro; IPR014144; LigD_PE_domain.
DR InterPro; IPR014145; LigD_pol_dom.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR014143; NHEJ_ligase_prk.
DR NCBIfam; TIGR02777; LigD_PE_dom; 1.
DR NCBIfam; TIGR02778; ligD_pol; 1.
DR NCBIfam; TIGR02779; NHEJ_ligase_lig; 1.
DR NCBIfam; TIGR02776; NHEJ_ligase_prk; 1.
DR PANTHER; PTHR42705; BIFUNCTIONAL NON-HOMOLOGOUS END JOINING PROTEIN LIGD; 1.
DR PANTHER; PTHR42705:SF2; MULTIFUNCTIONAL NON-HOMOLOGOUS END JOINING DNA REPAIR PROTEIN LIGD; 1.
DR Pfam; PF04679; DNA_ligase_A_C; 1.
DR Pfam; PF01068; DNA_ligase_A_M; 1.
DR Pfam; PF13298; LigD_N; 1.
DR Pfam; PF21686; LigD_Prim-Pol; 1.
DR SUPFAM; SSF56091; DNA ligase/mRNA capping enzyme, catalytic domain; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Manganese {ECO:0000256|ARBA:ARBA00023211};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000254305};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 343..458
FT /note="ATP-dependent DNA ligase family profile"
FT /evidence="ECO:0000259|PROSITE:PS50160"
FT REGION 188..212
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 191..212
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 855 AA; 97321 MW; 6F537377CFA1762B CRC64;
MKLSEYNKKR DFKKTAEPKG KVKKSGEVLH FVVQKHEASH LHFDWRLEID GVLVSWAVPK
GPSADPSVKR LAMHVEDHPM DYIDFEGNIP KGQYGGGTVM VWDTGSYLAE GADTVSQSKN
LIKEMYKEGN IKIIMQGKKL KGSYHLVRMS GKKQEWLLMK GKDDFANKKN FDQHSVLTGR
TLLEIQNDKS SETWESNKKA TPAKKGDKNH ILKENNDAAS DVLSFKSEDL APAKPRKTFP
REWKPQLATL TDAAFDDDDW IFENKYDGYR ALIQINGGKV NLVSRNGLSF NKKYESLVDS
FLAIKDDIIL DGEIVVEDSK GKSHFQWLQD YDENPGRGKL KCYMFDILYF NGYDLTGLSL
LQRKHILKAL LPESTTILYS DHTIGKGIQA LNKIAKKGGE GIIAKKLDSS YHVNKRSKDW
LKIKVTKEQE MVVGGYTEPK GIRNAFGSLL IGYYDDGKLI YSGKVGTGFN QASLEKLHAS
LMKIETTTCH FDEKPKIPNV HWVKPELVAQ VKFTEWTAGG SMRHPVFVAL RTDKKAKEVI
REKPLKKTKN AGQHNDVSRK DFKSDKVDIT NPDKIFWPKE GYTKGDVIAY YNVMADYVLK
YIKDRPQSLR RNPDGIKNQG FFQKDMSGKT PKWVKTRKLK SSSDGESVAY LVCNDKETLL
YMANLGCIEI NPWSSRIGSI NNPDYIIFDL DPNKASLQDL ITTAKKVKEI LDKLDIKGYL
KTSGGKGLHI FIPVKSNYTY RLTREFSRLI SETVYKALPD ITSLERMPKK RIGKVYLDFL
QNGKGKTMAC AYSLRPREGA TCSAPLEWSE LDHAFDIKNY NIKTMPRRIR EKGDLWNDFF
NDAIDLKTVL DKLKK
//
