ID A0A370F096_9FLAO Unreviewed; 944 AA.
AC A0A370F096;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=DEU42_10194 {ECO:0000313|EMBL:RDI15804.1};
OS Flavobacterium sp. AG291.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=2184000 {ECO:0000313|EMBL:RDI15804.1, ECO:0000313|Proteomes:UP000254305};
RN [1] {ECO:0000313|EMBL:RDI15804.1, ECO:0000313|Proteomes:UP000254305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AG291 {ECO:0000313|EMBL:RDI15804.1,
RC ECO:0000313|Proteomes:UP000254305};
RA Venturi V.;
RT "Genome sequencing of rice bacterial endophytes.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RDI15804.1}.
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DR EMBL; QQAR01000001; RDI15804.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A370F096; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000254305; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06139; DNA_polA_I_Ecoli_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000254305};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 5..266
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 346..531
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 701..908
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
FT REGION 293..333
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 562..589
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 944 AA; 106244 MW; 1E7B679D223B6FDF CRC64;
MSQQKRLFLL DAYALIFRGY YAFIKNPRIN SKGMDTSAIM GFMNSLLDVI KREKPDHLAV
AFDKGGSHVR TEMFTEYKAN RDETPEAIKV AVPYIQELLK AMHIPVIELE GCEADDLIGT
LAKQAEKQNY QVFMVTPDKD FAQLVSENIF MYRPARMGNG IEIWGVPEVL ERFEIERPEQ
VIDFLGMMGD AVDNIPGLPG VGEKTAKKFL KDYGTMENLL ANTHELKGKM KENIEANKEK
GLMSKVLATI ITDCDVTFHE EDFELSTPDI EKVEAIFNEL EFRRMAEQFQ KLFDDSTQPE
SGAPKAATPK ASSKNDQQFS LFGGGEPDSE TAETTTTGFY NTIANTDHFY QAVQGTMAIN
FLMRDLMKQT SVSFDTETTG VNPLIAELVG MSFSWEKGKG FYVPVPANRE EAQQVINLFL
PFFEDENIEK VGQNLKYDLE VLAQYGVTVK GKIFDTMIAH YLINPDMRHG MDILSETYLK
YSPVSIETLI GKKGKGQKSM RDVELDLIKE YAVEDADITL QLKEVFAPML ESTDTRKLFD
EIEIPLMPVL AAMEQEGINL DVPYLKELAA ELSADIKKLE QQIYETAGES FNLASPKQLG
DILFDKLKIG GAKQKKTKTG QYATGEEVLS YLAAENPIVA DILEWRQLVK LLNTYVEALP
LQVEPSTGRV HTDYMQTVAA TGRLSSNNPN LQNIPVRTER GRQIRKAFIA RNEDYTILSA
DYSQIELRII AALSGDPEMV SAFQKGEDIH ASTAAKVFNV ALNEVTREQR SHAKTVNFGI
VYGVSAFGLS NQTSLSRSES KELIDAYYKT YPRLKQYISE QVEIAREQGY VQTISGRRRY
LKDINSQNMV VRGGAERNAV NAPIQGSAAD IIKIAMINIQ KRLVDEGWKS KMLLQVHDEL
VFDVHNSELE KIKPMIKQEM ESAFKLDVPL EVDMGTGKNW LEAH
//
