ID A0A370H1Y3_9NOCA Unreviewed; 593 AA.
AC A0A370H1Y3;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex {ECO:0000256|RuleBase:RU003423};
DE EC=2.3.1.- {ECO:0000256|RuleBase:RU003423};
GN ORFNames=DFR68_106463 {ECO:0000313|EMBL:RDI50025.1};
OS Nocardia mexicana.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae;
OC Nocardia.
OX NCBI_TaxID=279262 {ECO:0000313|EMBL:RDI50025.1, ECO:0000313|Proteomes:UP000255355};
RN [1] {ECO:0000313|EMBL:RDI50025.1, ECO:0000313|Proteomes:UP000255355}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44952 {ECO:0000313|EMBL:RDI50025.1,
RC ECO:0000313|Proteomes:UP000255355};
RA Goeker M.;
RT "Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing the
RT most valuable type-strain genomes for metagenomic binning, comparative
RT biology and taxonomic classification.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=(R)-lipoate; Xref=ChEBI:CHEBI:83088;
CC Evidence={ECO:0000256|ARBA:ARBA00001938,
CC ECO:0000256|RuleBase:RU003423};
CC -!- SIMILARITY: Belongs to the 2-oxoacid dehydrogenase family.
CC {ECO:0000256|ARBA:ARBA00007317, ECO:0000256|RuleBase:RU003423}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RDI50025.1}.
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DR EMBL; QQAZ01000006; RDI50025.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A370H1Y3; -.
DR STRING; 1210089.GCA_001613165_04080; -.
DR OrthoDB; 9805770at2; -.
DR Proteomes; UP000255355; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR CDD; cd06849; lipoyl_domain; 2.
DR Gene3D; 2.40.50.100; -; 2.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR Gene3D; 4.10.320.10; E3-binding domain; 1.
DR InterPro; IPR003016; 2-oxoA_DH_lipoyl-BS.
DR InterPro; IPR001078; 2-oxoacid_DH_actylTfrase.
DR InterPro; IPR014276; 2-oxoglutarate_DH_E2.
DR InterPro; IPR000089; Biotin_lipoyl.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR036625; E3-bd_dom_sf.
DR InterPro; IPR004167; PSBD.
DR InterPro; IPR011053; Single_hybrid_motif.
DR NCBIfam; TIGR02927; SucB_Actino; 1.
DR PANTHER; PTHR43416:SF8; DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX; 1.
DR PANTHER; PTHR43416; DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED; 1.
DR Pfam; PF00198; 2-oxoacid_dh; 1.
DR Pfam; PF00364; Biotin_lipoyl; 2.
DR Pfam; PF02817; E3_binding; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 1.
DR SUPFAM; SSF47005; Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex; 1.
DR SUPFAM; SSF51230; Single hybrid motif; 2.
DR PROSITE; PS50968; BIOTINYL_LIPOYL; 2.
DR PROSITE; PS00189; LIPOYL; 2.
DR PROSITE; PS51826; PSBD; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000256|RuleBase:RU003423};
KW Lipoyl {ECO:0000256|ARBA:ARBA00022823, ECO:0000256|RuleBase:RU003423};
KW Reference proteome {ECO:0000313|Proteomes:UP000255355};
KW Transferase {ECO:0000256|RuleBase:RU003423}.
FT DOMAIN 2..77
FT /note="Lipoyl-binding"
FT /evidence="ECO:0000259|PROSITE:PS50968"
FT DOMAIN 131..206
FT /note="Lipoyl-binding"
FT /evidence="ECO:0000259|PROSITE:PS50968"
FT DOMAIN 281..318
FT /note="Peripheral subunit-binding (PSBD)"
FT /evidence="ECO:0000259|PROSITE:PS51826"
FT REGION 83..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 208..277
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 327..347
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 91..123
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 213..260
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 593 AA; 59553 MW; 2A744295305692F9 CRC64;
MAFSVQMPAL GESVTEGTVT RWLKQEGDTV EVDEPLLEVS TDKVDTEIPS PAAGVLTKIV
AQEDDVVEVG GELGVIGEAG EAAASAPAPA PEAAAPAPAP AEAPAPEPAQ QPAAAPAPEP
AAAPAPAAAS GTPVQMPELG ESVTEGTVTR WLKQVGDQIE VDEPLLEVST DKVDTEIPSP
VAGTLLEISA NEDDVVAVGG QLGVIGSGSP AAAPAPAPAP APAPEPAPAP AAAAPAPPPA
PAPAPAPAPA PAPAAPAPAA SAPAPAASAP ATSSGNGEAP YVTPLVRKLA AENNVDLSTV
KGSGVGGRIR KQDVLAAAES TQAPAAAPAA APAAAPKAPA PSAAPAGVRP QLQALRGTVQ
KASRIRQITA AKTRESLQTT AQLTQTHEVD VTKIAALRGK TKDAFKEREG VNLTFLPFFA
KAAVEALGVH PNVNASYNEE TKEITYHSAV HLGIAVDTDQ GLLSPVIHNA SDLSLAGLAR
AIADIAGRAR TGGLKPDELA GGTFTITNIG SQGALFDTPI LVPPQSAMLG TGAIVKRPMV
IADNGNEFIG IRSVCFLPLT YDHRLIDGAD AGRFLTTIKH RLEEAAFEAD LGL
//
