ID A0A370I3R9_9NOCA Unreviewed; 1165 AA.
AC A0A370I3R9;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 24-JAN-2024, entry version 17.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=DFR76_106249 {ECO:0000313|EMBL:RDI65379.1};
OS Nocardia pseudobrasiliensis.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae;
OC Nocardia.
OX NCBI_TaxID=45979 {ECO:0000313|EMBL:RDI65379.1, ECO:0000313|Proteomes:UP000254869};
RN [1] {ECO:0000313|EMBL:RDI65379.1, ECO:0000313|Proteomes:UP000254869}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44290 {ECO:0000313|EMBL:RDI65379.1,
RC ECO:0000313|Proteomes:UP000254869};
RA Goeker M.;
RT "Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing the
RT most valuable type-strain genomes for metagenomic binning, comparative
RT biology and taxonomic classification.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RDI65379.1}.
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DR EMBL; QQBC01000006; RDI65379.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A370I3R9; -.
DR STRING; 1210086.GCA_001613105_02191; -.
DR Proteomes; UP000254869; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd12113; PHP_PolIIIA_DnaE3; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 4: Predicted;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000254869};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1..58
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1165 AA; 127563 MW; 0426F30F8AB88E4B CRC64;
MLDGAAKITP LFKEAERLNM TAVGMTDHGN MYGASEFYNS AKKQGIKPII GIEAYIAPES
RFNTKRVLWG DPSQKSDDVS GSGSYTHMTM VAETATGLRN LFKLSSLASI EGQLGKWARM
DAEIIAEHAE GIIATTGCPS GEVQTRLRLG HDREALEAAA KWQEIFGKDN FFLEIMDHGL
SIERRVREGL LEVGRQLGIP PLATNDCHYV HESDSANHEA LLCIQTGKTL SDPTRFKFDG
SGYYLKSAEE MRAIWDAEVP GACDNTVLIG ERVQSYEDVW AHRDRMPIFP VPEGETQASW
LRKEVFRGLD RRFPGGVPDE YVRRADYELD VIIEMGFPAY FLVVGDLITY ARSVGISVGP
GRGSAAGSLV AYAMGITNID PIPHGLLFER FLNPERVSMP DIDIDFDDRR RGEMVRYATE
KWGTDRVAQV ITFGTIKTKA ALKDSARVQF GQPGFAIADQ ISKALPPPIM AKDIPLSGIM
DPEHERYKEA AEVRELINTN PDVNKIYETA RGLEGLIRNA GVHACAVIMS SEPLTDAIPV
WKRAQDGAII TGWDYPSCEA IGLLKMDFLG LRNLTVIGDA LVNIKANRGI ELDLDTLPLE
DPPTYELLAR GDTLGVFQLD GGAMRDLLRR MQPTGFEDIV AVLALYRPGP MGMNAHNDYA
DRKNKRQEVK PIHPELEEPL KDILADTYGL IVYQEQIMQI AQKVAGYSLG RADILRRAMG
KKKAEVLEAE FEGFEKGMLE NGFSKPAIKA LWDTILPFAG YAFNKSHAAG YGLVSFWTAY
LKANYPAEYM AGLLTSVGDD KDKAAVYLAD CRRLGIQVLP PDVNESELEF ASVGNDIRFG
MGAVRNVGAN VVASIIAARK EKSKFTDFSD YLNKIDTVAC TKKVTESLIK AGAFDSLNHP
RKGLLLVHSD AIDAVMATKK AEAIGQFDLF GGLDDSDESM ASVFNVKVAD EEWDTKHKLA
LEREMLGLYV SGHPLDGVAH VLSAQADTAI PNILEGNLQD GAQVTVGGIL ASVTRRINKN
GMPWASAQLE DLSGGIEVLF FPQAYSAFGM DVVEDAVVLV KARVSARDDR ISLIANDLVV
PDLSAIGMEK PLAVTMPTRM CTPDKIKALQ RVLRSHPGPA DVHIRHVGAR EKTTLLKLDD
SLRVSPSSAL MGDLKALLGP GCLAG
//
