ID A0A371ASZ0_9FIRM Unreviewed; 712 AA.
AC A0A371ASZ0;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=Alpha-galactosidase {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
DE EC=3.2.1.22 {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
GN ORFNames=DWV06_14790 {ECO:0000313|EMBL:RDU22669.1};
OS Anaerosacchariphilus polymeriproducens.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae;
OC Anaerosacchariphilus.
OX NCBI_TaxID=1812858 {ECO:0000313|EMBL:RDU22669.1, ECO:0000313|Proteomes:UP000255036};
RN [1] {ECO:0000313|EMBL:RDU22669.1, ECO:0000313|Proteomes:UP000255036}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MCWD5 {ECO:0000313|EMBL:RDU22669.1,
RC ECO:0000313|Proteomes:UP000255036};
RA Kim W., Yang S.-H., Oh J., Lee J.-H., Kwon K.K.;
RT "Anaerosacharophilus polymeroproducens gen. nov. sp. nov., an anaerobic
RT bacterium isolated from salt field.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|ARBA:ARBA00001255,
CC ECO:0000256|PIRNR:PIRNR005536};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase.
CC {ECO:0000256|PIRNR:PIRNR005536}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RDU22669.1}.
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DR EMBL; QRCT01000049; RDU22669.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A371ASZ0; -.
DR OrthoDB; 9758822at2; -.
DR Proteomes; UP000255036; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd14791; GH36; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 2.70.98.60; alpha-galactosidase from lactobacil brevis; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR038417; Alpga-gal_N_sf.
DR InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR InterPro; IPR002252; Glyco_hydro_36.
DR InterPro; IPR031705; Glyco_hydro_36_C.
DR InterPro; IPR031704; Glyco_hydro_36_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43053:SF3; ALPHA-GALACTOSIDASE C-RELATED; 1.
DR PANTHER; PTHR43053; GLYCOSIDASE FAMILY 31; 1.
DR Pfam; PF16874; Glyco_hydro_36C; 1.
DR Pfam; PF16875; Glyco_hydro_36N; 1.
DR Pfam; PF02065; Melibiase; 1.
DR PIRSF; PIRSF005536; Agal; 1.
DR PRINTS; PR00743; GLHYDRLASE36.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR005536};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005536};
KW Reference proteome {ECO:0000313|Proteomes:UP000255036}.
FT DOMAIN 10..266
FT /note="Glycosyl hydrolase family 36 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16875"
FT DOMAIN 631..705
FT /note="Glycosyl hydrolase family 36 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16874"
FT ACT_SITE 459
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT ACT_SITE 529
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
SQ SEQUENCE 712 AA; 82006 MW; 57C2E79164EF2896 CRC64;
MYQMCVDKLG YLLHLYYGST IENEDLDYLI RYIDRACSGQ PYDALDDRTY SLDILPQEYS
TFGSGDYRTS SILVENSDGS QTVDLRFKEF TIYEGKYKLQ GLPSVYAKEE ADTLEIILED
KHTNMEVVLL YGVFKNLDVI TRAVRVKNNG AESVYLNRVL STCLDFYENK YDFISFYGKH
MLERQLERRH LTHGKTSVES VRGVSSHHQN PFVILCDRKA DEDIGDCYGF SFVYSGNFLA
ETEVDQINQT RFVMGINPTG FRYCLKSQEE FIAPEVVMVF SQNGLGELSR IYHDVYRDHL
CRGNYAKKQR PVLINNWEAT YFDFDEQKIL DIASMAADVG IEMLVMDDGW FGKRNNDLAG
LGDWVVNHDK IPSGINGLCQ KINDLGIKMG LWFEPEMVNE DSDLYRAHPD WCIKVPERSP
VRARYQLVLD VSRKEVRDYI FDLITKVLDG TNIEYIKWDM NRNLTDLYSA GLSKESQGEL
FHRYVLGVYD LMERLIQRYP NILLENCSGG GGRFDPGMLY YSPQIWCSDN TDAIDRIKIQ
YGTSFGYPIS SMGAHVSVTP NHGNGRCTDF KTRGIVAMAG TFGYELDITK LSDEEKDMIQ
SQIEEFKRNY DVIQYGDYYR LTDVMNNHDF AAWQFVDKQK TKSLCSYVSL TAKANAPTVR
LRLKGLDASK KYRINKEDKI YSGDLLMKAG FVVPETFGDY KAINFEILEV QY
//
