ID A0A371EMX5_MUCPR Unreviewed; 410 AA.
AC A0A371EMX5;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=U-box domain-containing protein {ECO:0000256|RuleBase:RU369093};
DE EC=2.3.2.27 {ECO:0000256|RuleBase:RU369093};
DE AltName: Full=RING-type E3 ubiquitin transferase PUB {ECO:0000256|RuleBase:RU369093};
DE Flags: Fragment;
GN Name=PUB24 {ECO:0000313|EMBL:RDX67334.1};
GN ORFNames=CR513_53804 {ECO:0000313|EMBL:RDX67334.1};
OS Mucuna pruriens (Velvet bean) (Dolichos pruriens).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Mucuna.
OX NCBI_TaxID=157652 {ECO:0000313|EMBL:RDX67334.1, ECO:0000313|Proteomes:UP000257109};
RN [1] {ECO:0000313|EMBL:RDX67334.1, ECO:0000313|Proteomes:UP000257109}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jos {ECO:0000313|Proteomes:UP000257109};
RC TISSUE=Seed {ECO:0000313|EMBL:RDX67334.1};
RA Nnadi N.E., Vos R., Hasami M.H., Devisetty U.K., Aguiy J.C.;
RT "Draft genome of Mucuna pruriens seed.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase.
CC {ECO:0000256|RuleBase:RU369093}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900,
CC ECO:0000256|RuleBase:RU369093};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906, ECO:0000256|RuleBase:RU369093}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RDX67334.1}.
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DR EMBL; QJKJ01013038; RDX67334.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A371EMX5; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000257109; Unassembled WGS sequence.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniRule.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR045185; PUB22/23/24-like.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR22849:SF128; OS12G0161100 PROTEIN; 1.
DR PANTHER; PTHR22849; WDSAM1 PROTEIN; 1.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 4: Predicted;
KW Transferase {ECO:0000256|RuleBase:RU369093};
KW Ubl conjugation pathway {ECO:0000256|RuleBase:RU369093}.
FT DOMAIN 5..82
FT /note="U-box"
FT /evidence="ECO:0000259|PROSITE:PS51698"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:RDX67334.1"
SQ SEQUENCE 410 AA; 46303 MW; 3C760FC1E1EE6445 CRC64;
MAEVEIPQYF LCPISFQIME DPVTTLTGIT YDRESIEQWL LKARDCVCPV TKQALPRSSQ
YLTPNHTLRR LIQAWRSANT ANGVDQIPTP KTPLSTSHVQ KLVKDLEVLN AYQTSLEKLL
VLATQERNRT CMAEAGVAKG VVKVIKKNFK EGNSNITCIE EALRIVHLLW NNDHNNSMRT
LVGENLDFIN SLTWILQLHL DNNMKMVNEA MFLLKLTIEV ADPTLLGSLS LEFFKVMVRV
LKKRALPKQA IKSSLHVLIE TSSLSRNRTK IVEAGAVTEL IELELEKPEK NMTELILNLL
AHLCSCAEGR EQFLRHAAGI AVVSKRLLRV SAATDERAIH VFSVIAKSSA SNEVVLEMLR
VGAVSKLCMV MQADCASYLK ERTRDILRLH SKVWNNSPCI QVYLLTRHQR
//
