ID A0A371G9N5_MUCPR Unreviewed; 398 AA.
AC A0A371G9N5;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE SubName: Full=Naringenin,2-oxoglutarate 3-dioxygenase {ECO:0000313|EMBL:RDX87239.1};
DE Flags: Fragment;
GN Name=F3H {ECO:0000313|EMBL:RDX87239.1};
GN ORFNames=CR513_31321 {ECO:0000313|EMBL:RDX87239.1};
OS Mucuna pruriens (Velvet bean) (Dolichos pruriens).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Mucuna.
OX NCBI_TaxID=157652 {ECO:0000313|EMBL:RDX87239.1, ECO:0000313|Proteomes:UP000257109};
RN [1] {ECO:0000313|EMBL:RDX87239.1, ECO:0000313|Proteomes:UP000257109}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jos {ECO:0000313|Proteomes:UP000257109};
RC TISSUE=Seed {ECO:0000313|EMBL:RDX87239.1};
RA Nnadi N.E., Vos R., Hasami M.H., Devisetty U.K., Aguiy J.C.;
RT "Draft genome of Mucuna pruriens seed.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=L-ascorbate; Xref=ChEBI:CHEBI:38290;
CC Evidence={ECO:0000256|ARBA:ARBA00001961};
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004966}.
CC -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC family. {ECO:0000256|ARBA:ARBA00008056, ECO:0000256|RuleBase:RU003682}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RDX87239.1}.
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DR EMBL; QJKJ01006280; RDX87239.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A371G9N5; -.
DR STRING; 157652.A0A371G9N5; -.
DR Proteomes; UP000257109; Unassembled WGS sequence.
DR GO; GO:0051213; F:dioxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-KW.
DR InterPro; IPR026992; DIOX_N.
DR InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR InterPro; IPR027443; IPNS-like_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR PANTHER; PTHR47991:SF60; FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR47991; OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE; 1.
DR Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR Pfam; PF14226; DIOX_N; 1.
DR SUPFAM; SSF51197; Clavaminate synthase-like; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Dioxygenase {ECO:0000313|EMBL:RDX87239.1};
KW Flavonoid biosynthesis {ECO:0000256|ARBA:ARBA00023241};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU003682};
KW Metal-binding {ECO:0000256|RuleBase:RU003682};
KW Oxidoreductase {ECO:0000256|RuleBase:RU003682}.
FT DOMAIN 191..303
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000259|PROSITE:PS51471"
FT COILED 342..390
FT /evidence="ECO:0000256|SAM:Coils"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:RDX87239.1"
SQ SEQUENCE 398 AA; 45287 MW; 75071DEBA306835D CRC64;
MAPAKTLTYL AQEKTLESSF VRDEDERPKV AYNQFSDEIP VISLAGIDDV DGRRAEICDK
IVKACENWGI FQVVDHGVDQ NLVTEMTRLA KEFFALPPED KLRFDMSGGK KGGFIVSSHL
QGESVQDWRE IVTYFSYPIR ERDYSRWPDK PEGWKSVTEE YSEKLMGLAC KLLEVLSEAM
GLEKEALTKA CVDMDQKVVV NYYPKCPQPD LTLGLKRHTD PGTITLLLQD QVGGLQATRD
NGNTWITVQP IQGAFVVNLG DHGHVSMPSF FNFLSNGRFK NADHQAVVNS NSSRLSIATF
QNPAPNATVY PLKVREGEKP VLEEPITFAE MYRRKMSKDL EIARMKKLAK EKQLQLDLEK
KEKELKHLDL EKKEKELQHL DQLEKEKLQA KPLKEILA
//
