ID   A0A371I8A2_MUCPR        Unreviewed;       398 AA.
AC   A0A371I8A2;
DT   07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT   07-NOV-2018, sequence version 1.
DT   27-MAR-2024, entry version 18.
DE   SubName: Full=Long chain acyl-CoA synthetase 8 {ECO:0000313|EMBL:RDY11164.1};
DE   Flags: Fragment;
GN   Name=LACS8 {ECO:0000313|EMBL:RDY11164.1};
GN   ORFNames=CR513_04200 {ECO:0000313|EMBL:RDY11164.1};
OS   Mucuna pruriens (Velvet bean) (Dolichos pruriens).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Mucuna.
OX   NCBI_TaxID=157652 {ECO:0000313|EMBL:RDY11164.1, ECO:0000313|Proteomes:UP000257109};
RN   [1] {ECO:0000313|EMBL:RDY11164.1, ECO:0000313|Proteomes:UP000257109}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Jos {ECO:0000313|Proteomes:UP000257109};
RC   TISSUE=Seed {ECO:0000313|EMBL:RDY11164.1};
RA   Nnadi N.E., Vos R., Hasami M.H., Devisetty U.K., Aguiy J.C.;
RT   "Draft genome of Mucuna pruriens seed.";
RL   Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RDY11164.1}.
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DR   EMBL; QJKJ01000695; RDY11164.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A371I8A2; -.
DR   STRING; 157652.A0A371I8A2; -.
DR   Proteomes; UP000257109; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004467; F:long-chain fatty acid-CoA ligase activity; IEA:UniProt.
DR   Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR   InterPro; IPR042099; ANL_N_sf.
DR   PANTHER; PTHR43272:SF92; LONG CHAIN ACYL-COA SYNTHETASE 8; 1.
DR   PANTHER; PTHR43272; LONG-CHAIN-FATTY-ACID--COA LIGASE; 1.
DR   Pfam; PF00501; AMP-binding; 1.
DR   SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
PE   4: Predicted;
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        27..49
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          129..393
FT                   /note="AMP-dependent synthetase/ligase"
FT                   /evidence="ECO:0000259|Pfam:PF00501"
FT   NON_TER         398
FT                   /evidence="ECO:0000313|EMBL:RDY11164.1"
SQ   SEQUENCE   398 AA;  43651 MW;  C9F83125CAD58AA9 CRC64;
     MVDPKDSFSW LKSLNINDVF VWKDHGVYGA LAAIVISILV PVLFSALFFG KKKGKVRGVP
     VDVGGEAGYA VRNARKTEMV EVPWIGAPTM AHLFEQSCNK YTHNRFLGKR KLIQREFVSS
     SDGRKFEKLH LGDYEWETYG EVFARASNFA SGLLKLGHNI DSRVAIFSDT QAEWLIALQG
     CFRQNVTVVT IYASLGEDAL IHSLNETEVS TLICESKLLK KLNAIRSRLT YIQNIIYFED
     DNKEEDAFSE ISSSWSISSF SEVEKLGKEN PVEPSLPSKN AIAVIMYTSG STGLPKGVMI
     THGNIVATTA AVMTVIPNLG SKDVYLAYLP LAHVFEMAAE SVMLAAGCAI GYSSPLTLTD
     TSNKIKKGTK GDATVLKPTL LTAVPAILDR IREGVVKK
//