ID A0A371IQP8_9FIRM Unreviewed; 270 AA.
AC A0A371IQP8;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase {ECO:0000256|ARBA:ARBA00019161};
DE EC=2.7.1.49 {ECO:0000256|ARBA:ARBA00012135};
DE EC=2.7.4.7 {ECO:0000256|ARBA:ARBA00012963};
DE AltName: Full=Hydroxymethylpyrimidine kinase {ECO:0000256|ARBA:ARBA00042102};
DE AltName: Full=Hydroxymethylpyrimidine phosphate kinase {ECO:0000256|ARBA:ARBA00043176};
GN Name=thiD {ECO:0000313|EMBL:RDY22788.1};
GN ORFNames=CHF27_011460 {ECO:0000313|EMBL:RDY22788.1};
OS Romboutsia maritimum.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Peptostreptococcaceae;
OC Romboutsia.
OX NCBI_TaxID=2020948 {ECO:0000313|EMBL:RDY22788.1, ECO:0000313|Proteomes:UP000243494};
RN [1] {ECO:0000313|EMBL:RDY22788.1, ECO:0000313|Proteomes:UP000243494}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCRI-22766 {ECO:0000313|EMBL:RDY22788.1,
RC ECO:0000313|Proteomes:UP000243494};
RX PubMed=29025937;
RA Maheux A.F., Boudreau D.K., Berube E., Boissinot M., Raymond F.,
RA Brodeur S., Corbeil J., Brightwell G., Broda D., Omar R.F., Bergeron M.G.;
RT "Draft Genome Sequence of Romboutsia maritimum sp. nov. Strain CCRI-
RT 22766(T), Isolated from Coastal Estuarine Mud.";
RL Genome Announc. 5:e01044-17(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ATP = 4-
CC amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + ADP;
CC Xref=Rhea:RHEA:19893, ChEBI:CHEBI:30616, ChEBI:CHEBI:57841,
CC ChEBI:CHEBI:58354, ChEBI:CHEBI:456216; EC=2.7.4.7;
CC Evidence={ECO:0000256|ARBA:ARBA00000565};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-amino-5-hydroxymethyl-2-methylpyrimidine + ATP = 4-amino-2-
CC methyl-5-(phosphooxymethyl)pyrimidine + ADP + H(+);
CC Xref=Rhea:RHEA:23096, ChEBI:CHEBI:15378, ChEBI:CHEBI:16892,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58354, ChEBI:CHEBI:456216;
CC EC=2.7.1.49; Evidence={ECO:0000256|ARBA:ARBA00000151};
CC -!- PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-
CC amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-
CC ribosyl)imidazole: step 2/3. {ECO:0000256|ARBA:ARBA00037917}.
CC -!- SIMILARITY: Belongs to the ThiD family.
CC {ECO:0000256|ARBA:ARBA00009879}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RDY22788.1}.
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DR EMBL; NOJZ02000026; RDY22788.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A371IQP8; -.
DR OrthoDB; 9810880at2; -.
DR Proteomes; UP000243494; Unassembled WGS sequence.
DR GO; GO:0008902; F:hydroxymethylpyrimidine kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0008972; F:phosphomethylpyrimidine kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0009228; P:thiamine biosynthetic process; IEA:InterPro.
DR CDD; cd01169; HMPP_kinase; 1.
DR Gene3D; 3.40.1190.20; -; 1.
DR InterPro; IPR004399; HMP/HMP-P_kinase_dom.
DR InterPro; IPR013749; PM/HMP-P_kinase-1.
DR InterPro; IPR029056; Ribokinase-like.
DR NCBIfam; TIGR00097; HMP-P_kinase; 1.
DR PANTHER; PTHR20858:SF17; HYDROXYMETHYLPYRIMIDINE_PHOSPHOMETHYLPYRIMIDINE KINASE THI20-RELATED; 1.
DR PANTHER; PTHR20858; PHOSPHOMETHYLPYRIMIDINE KINASE; 1.
DR Pfam; PF08543; Phos_pyr_kin; 1.
DR SUPFAM; SSF53613; Ribokinase-like; 1.
PE 3: Inferred from homology;
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000313|EMBL:RDY22788.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000243494};
KW Transferase {ECO:0000313|EMBL:RDY22788.1}.
FT DOMAIN 15..258
FT /note="Pyridoxamine kinase/Phosphomethylpyrimidine kinase"
FT /evidence="ECO:0000259|Pfam:PF08543"
SQ SEQUENCE 270 AA; 29742 MW; 559BEBED76A34B03 CRC64;
MRNYKIPTLT IAGSDSSGGA GIQADLKTFS AIGTYGMSVI TAITAQNTQG VFEVEELSKK
IIRKQIEVVF EDITPKAVKI GMVSSPEIIL EIVNSLKNYN LEYLVVDPVM ISKSGYSLLR
PQAKENLMKY LIPMAYIITP NIPEAEEITG MKVNNIDDMK IVGEKILELG PKYVLMKGGH
LDGDAIDVLI GEDIFEIYKS KRIDRKNTHG TGCTLSSAIT SHLALGYGIK ESIKLSKEYI
TQAIKYSFDI GHGVGPVQHF YKFTKVEGEI
//
