ID A0A371P5L5_9BACL Unreviewed; 762 AA.
AC A0A371P5L5;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=DX130_22640 {ECO:0000313|EMBL:REK71244.1};
OS Paenibacillus paeoniae.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=2292705 {ECO:0000313|EMBL:REK71244.1, ECO:0000313|Proteomes:UP000261905};
RN [1] {ECO:0000313|EMBL:REK71244.1, ECO:0000313|Proteomes:UP000261905}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M4BSY-1 {ECO:0000313|EMBL:REK71244.1,
RC ECO:0000313|Proteomes:UP000261905};
RA Tuo L.;
RT "Paenibacillus sp. M4BSY-1, whole genome shotgun sequence.";
RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:REK71244.1}.
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DR EMBL; QUBQ01000006; REK71244.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A371P5L5; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000261905; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000261905}.
FT DOMAIN 17..313
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 314..576
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 662..688
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 664..688
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 38..45
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 762 AA; 86901 MW; 50A6FB672D91FE7E CRC64;
MGKLAPFLER KHKELGVQLN EVQRQAVEQA EGPLLLLASP GSGKTTTLIM RIGYLIEELG
TDPARIKAVT FSRASARDMK DRFTRFFPGL QPVDFSTIHS LAFEIVRTHF YKTRTAYDII
EGALEIEEED EAQGTVKPLP LHKRIILRNL FKDIVGENAT DDAMEELTTY ISYVKNKMLP
PEQWASAKVD VPQADRILHE YEAFKRSGTP KLLIDFDDML TIANDVLEKD GAMLRKLQSR
YDYVMTDESQ DTSMVQHAII EKLAMGHRNL CVVADDDQSI YSWRGAEPSY LLRFKDVYPD
AVMLYMVQNY RSSPEIVQTA NTFIKRNKKR YEKEMFTHNA SHEPIAFKTL RDYREQAKYV
VREIAKMEKL SEAAVLYRNN SSSIALMNDF DRAGIPFYMK DADNRFFSHW VIEDVLNFMR
MSYTDKRADI LENIHLKFNG YISKHQMSVL KSIDNGESVF DNLLTIVQLH DYQIKPLQEA
KETFREMNSM PPLPAIRVIR TRLGYEKALE KICERLGFRK EYLIGILNTL EDIAEGLETM
ADFAARLKYL EKVLKGAKSR KGQNAVTFST FHSAKGLEFD HVYMVDLVEG IIPGSEDGKK
EANGESSSME EAVRLFYVGM TRARKTLEMV TYGQRDGEQA TESSFMTAIK DIVDPERITA
RTTAASERRS ASFSEGESTR ALKGMKSTSF TARREPIQAE LNPHAVKNPA EVTVGANVRH
RVFGVGCVER ADNESIMIRF SENNVKTLAM AMCMERGLLE LV
//
