ID A0A372EWB9_9RHOB Unreviewed; 1148 AA.
AC A0A372EWB9;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969,
GN ECO:0000313|EMBL:RFP89045.1};
GN ORFNames=DZK27_06585 {ECO:0000313|EMBL:RFP89045.1};
OS Rhodobacteraceae bacterium 63075.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Paracoccaceae.
OX NCBI_TaxID=2301226 {ECO:0000313|EMBL:RFP89045.1, ECO:0000313|Proteomes:UP000264392};
RN [1] {ECO:0000313|Proteomes:UP000264392}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=63075 {ECO:0000313|Proteomes:UP000264392};
RA Han S.;
RT "Wenzhouxiangella salilacus sp. nov., a novel bacterium isolated from a
RT saline lake in Xinjiang Province, China.";
RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RFP89045.1}.
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DR EMBL; QVMW01000005; RFP89045.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A372EWB9; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000264392; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000264392}.
FT DOMAIN 608..769
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 790..944
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1148 AA; 126937 MW; 849E006E6B7E76F9 CRC64;
MAEHLTVSGA PEGFDAQLVL REAEKTGAVC HIARDDKRMA AMKAALAFFA PELPVIEFPA
WDCLPYDRVS PNPDISAARM ATLAALVHEM PQRFVLLTTL SAAMQYLPAR EVLREAAFAA
RVGERVNEAA LRDFLVRMGF SQSPTVTEPG DYAIRGGIID IFSPGEGGPV RLDFFGDVLD
GVRRFDPVSQ RTTETLDKVE LAPVSEVILD EAAITRFRQN YRLEFGAAGS DDPLYEAVSA
GRKHAGIEHW LPFFHARLET LFDYLGSASL TMDEGMESLR TARWETIEDQ YETRRLAMTE
KARMDTVYKP VPAEQLYLDE AAWSAALEGR RTLKFNALPL PTGPGVTDTG ARIGRNFAPE
RQMENVNLFS VLKDHIEARM AAGPVLVASY SDGARERLEG LLEDEGLIGA VTVKQAGRIG
AHGLHLAVWP LERGFEAPGL TVIAEQDVLG DRLIRQPKRK RRAENFLTEA QSLSPGDLIV
HVDHGIGRYH GLEVITAAGA AHECLKLEYA EGARLYLPVE NIELLSRYGH EEGLLDKLGG
GAWQAKKAKL KERIREMAER LIRVAAERAL RRAPVMELQD HAWEEFAARF PYQETDDQLG
AIEDVLADLG SGQPMDRLIC GDVGFGKTEV AMRAAFVAAM QGAQVAVIAP TTLLARQHYK
SFAERFRGFP INVRPLSRFV GQKEAKLTRE GIKRGTVDIA IGTHALLAKD IQFKDLGLLI
IDEEQHFGVA HKERLKQLRS DVHVLTLTAT PIPRTLQLSL SGVRDLSIIG TPPVDRLAIR
TYISEFDGVT IREALLREHY RGGQSFYVAP RVNDLPEIEE FLKEHVPEVS YVIAHGQMAA
GELDSRMNAF YDGKYDVLLA TTIVESGLDI PTANTMIIHR ADMFGLAQLY QIRGRVGRSK
TRAYAYLTTR PRKPLSETAE KRLRVLGSLD TLGAGFTLAS QDLDIRGAGN LLGEEQSGQM
RDVGYELYQS MLEEAIAKIR SGEAEGLTDE GDWSPQINLG VPVLIPEDYV PDLDVRLGLY
RRLSGLSGKV EMEGFAAELI DRFGKLPREV NTLLLVVRIK EMCKKAGIAK LDGGPKGATI
QFHNDKFAAP AGLVEFIREQ QGLAKVKDNK IVVRRDWKKD GDKIKGAFAI ARDLAAKVAA
HEKDKKDA
//
