ID   A0A372G1M0_9ACTN        Unreviewed;       699 AA.
AC   A0A372G1M0;
DT   07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT   07-NOV-2018, sequence version 1.
DT   24-JAN-2024, entry version 19.
DE   RecName: Full=cellulase {ECO:0000256|ARBA:ARBA00012601};
DE            EC=3.2.1.4 {ECO:0000256|ARBA:ARBA00012601};
GN   ORFNames=D0Q02_09285 {ECO:0000313|EMBL:RFS46951.1};
OS   Micromonospora craniellae.
OC   Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC   Micromonosporaceae; Micromonospora.
OX   NCBI_TaxID=2294034 {ECO:0000313|EMBL:RFS46951.1, ECO:0000313|Proteomes:UP000262621};
RN   [1] {ECO:0000313|EMBL:RFS46951.1, ECO:0000313|Proteomes:UP000262621}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LHW63014 {ECO:0000313|EMBL:RFS46951.1,
RC   ECO:0000313|Proteomes:UP000262621};
RA   Li L., Lin H.W.;
RT   "Verrucosispora craniellae sp. nov., isolated from a marine sponge in the
RT   South China Sea.";
RL   Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00000966};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RFS46951.1}.
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DR   EMBL; QVFU01000006; RFS46951.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A372G1M0; -.
DR   OrthoDB; 9800955at2; -.
DR   Proteomes; UP000262621; Unassembled WGS sequence.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030247; F:polysaccharide binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.60.40.290; -; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR005102; Carbo-bd_X2.
DR   InterPro; IPR001919; CBD2.
DR   InterPro; IPR008965; CBM2/CBM3_carb-bd_dom_sf.
DR   InterPro; IPR012291; CBM2_carb-bd_dom_sf.
DR   InterPro; IPR040946; CBM46.
DR   InterPro; IPR001547; Glyco_hydro_5.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   PANTHER; PTHR31297:SF17; ENDOGLUCANASE; 1.
DR   PANTHER; PTHR31297; GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B; 1.
DR   Pfam; PF18448; CBM46; 1.
DR   Pfam; PF00553; CBM_2; 1.
DR   Pfam; PF03442; CBM_X2; 1.
DR   Pfam; PF00150; Cellulase; 1.
DR   SMART; SM00637; CBD_II; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF49384; Carbohydrate-binding domain; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   PROSITE; PS51173; CBM2; 1.
PE   4: Predicted;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00023295};
KW   Reference proteome {ECO:0000313|Proteomes:UP000262621};
KW   Signal {ECO:0000256|ARBA:ARBA00022729}.
FT   DOMAIN          36..145
FT                   /note="CBM2"
FT                   /evidence="ECO:0000259|PROSITE:PS51173"
FT   REGION          142..169
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        149..169
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   699 AA;  75795 MW;  04F3DAC8ED71A5C7 CRC64;
     MAGLRNTLRR TRWRFGLVTS GIAVLLAGVG LVNAGAAHAA AGCRVVYSAP SQWPGGFTAN
     VDLTNLGDPV NGWRLTWTFP SGQRVTQAWN ATVTSSGNDV TATNVGYNAA IGTNQSVSFG
     FNGSYTGTNT APTSFALNGV TCTGSVGPTT PPPTTPPPTT PPPTTPPPTG DAMAYVAAMQ
     PGWNLGNTLD AVGDDETAWG NPRVTAAQLD AIRAQGFNSI RLPITWSNRH GPAPSYTIDA
     AWLNRIKEVV GWALDDGFYV MINIHHDSWQ WIHEMPTNRT TVLNRYNALW TQIAAAFRDS
     SPKLHFESVN EPQFANSSGD AQNAQLLHEL NTSFHRIVRA SGGGNATRML VLPTLHTSSD
     QPRIDELVST FTQLNDRNLI ATVHFYGYWP FSVNVAGGTR FDAVTQQDLV DRFDRVHNAF
     VARNIPVVIG EYGLLGFDRH TGTVQQGEKL KFFEFLGHYA RTKRITTQLW DNGQHFDRVA
     FRWKDAELFA QIKSSWTTRS GTASADQVYV PRTGSVTAKS LTLNLNGTTF SGLRQGTTNL
     SQGADYTVSG NQLTLTASAL TRLVGSRAYG VNATLHARFS TGVPWRIDII TYDPPVLSNA
     TGSTEAFAIP TQFRGDQLAT MEAKYADGSN AGPHDWTSYK EFDVTFAPDY TGNRISLTPT
     FFGEVRDGAP VTLTFHFWSG AQVTYRVTKS GSSVTGVTG
//