ID A0A373DSW7_9MICO Unreviewed; 874 AA.
AC A0A373DSW7;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=DNA polymerase I {ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=D1J51_04615 {ECO:0000313|EMBL:RGE22496.1};
OS Leucobacter sp. wl10.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Leucobacter.
OX NCBI_TaxID=2304677 {ECO:0000313|EMBL:RGE22496.1, ECO:0000313|Proteomes:UP000263010};
RN [1] {ECO:0000313|EMBL:RGE22496.1, ECO:0000313|Proteomes:UP000263010}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=wl10 {ECO:0000313|Proteomes:UP000263010};
RA Liu H.;
RT "Leucobacter sp. wl 10 isolated from wastewater.";
RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RGE22496.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; QWBV01000005; RGE22496.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A373DSW7; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000263010; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:UniProt.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000263010};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 1..259
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 630..837
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 874 AA; 96788 MW; 966EFC485DB50376 CRC64;
MIIDGHSLAF RAFYALPVDS FQTQTGQHTN AIHGFIAMLI NLLGNESPDA LAVAFDISRH
SFRTEEYPEY KGTRGETPPE FKGQVPLLQE ALHAMGIRTL EKENFEADDI LATLATRGAE
AGYRVLVVSG DRDTIQLVDD RITLLYPSKQ GVSELTRYDA QKVMDRYGIR PEQYPEIAAL
VGETSDNLPG IPRVGEKTAV KWITQFGSLE EILRRRDEIG GKVGESLREH AHLAERNRRL
NRLVRDVDLD LTLDDLKRGE IDMAAVQQVF AKLEFRTLLQ RVGKLAGAEV PSGGAATAAA
SLVPERPEAK NLIDEELGDW LAKADHPAVL IRESDAGFEV GIATPESSVL FQWQPGGRDY
ALFEQWLASD SPKIFFDAKQ QIGVAARAGA AIGGIDGDVL LAAWLLRPAT AEKDIAEAAF
RFLGEQVPEG DPNQLVPDES SVADAAALAW YVVRTHAAAV ERFQSRTVDI YRDIELPLVS
VLAELEQRGV QIDLPLLEAH NAELQARVLD LEQQAFTAIG REVNLSSPKQ LQEVLFEQLD
MPKTRKTKSG YTTDAAALAE LQAKKPHPFL DALLAHRDAN KLRQMVETLI KAVQRDGRVH
TTFLQTGAST GRLASTDPNL QNIPVRSEEG RRIREGFIHA PEYETLMTAD YSQIEMRIMA
HLSGDAGLIE AFRQGEDLHR FVGSRIFGVP PEEVTSEMRA KVKAMSYGLA YGLSAFGLSK
QLGITGAEAK QLMVDYFERF GGVRDYLRSV VDQAKRDTFT ETIFGRRRPF PDLASPNRIL
RENAERAALN SPIQGSAADI IKRAMIQVER RMRDAELNSR MLLQIHDELM FEVAEGEWEA
LEAIVREEMA GAAELSVPLE VQVGHGANWN AAAH
//
