ID A0A373LG12_9FIRM Unreviewed; 787 AA.
AC A0A373LG12;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=alpha-galactosidase {ECO:0000256|ARBA:ARBA00012755};
DE EC=3.2.1.22 {ECO:0000256|ARBA:ARBA00012755};
GN ORFNames=DW006_10590 {ECO:0000313|EMBL:RGF48746.1};
OS Eubacterium sp. AF36-5BH.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Eubacteriaceae;
OC Eubacterium.
OX NCBI_TaxID=2293108 {ECO:0000313|EMBL:RGF48746.1, ECO:0000313|Proteomes:UP000262191};
RN [1] {ECO:0000313|EMBL:RGF48746.1, ECO:0000313|Proteomes:UP000262191}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF36-5BH {ECO:0000313|EMBL:RGF48746.1,
RC ECO:0000313|Proteomes:UP000262191};
RA Zou Y., Xue W., Luo G.;
RT "A genome reference for cultivated species of the human gut microbiota.";
RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|ARBA:ARBA00001255};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RGF48746.1}.
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DR EMBL; QTVG01000010; RGF48746.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A373LG12; -.
DR OrthoDB; 9758822at2; -.
DR Proteomes; UP000262191; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd14791; GH36; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 2.70.98.60; alpha-galactosidase from lactobacil brevis; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR038417; Alpga-gal_N_sf.
DR InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR InterPro; IPR002252; Glyco_hydro_36.
DR InterPro; IPR031705; Glyco_hydro_36_C.
DR InterPro; IPR031704; Glyco_hydro_36_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43053:SF3; ALPHA-GALACTOSIDASE C-RELATED; 1.
DR PANTHER; PTHR43053; GLYCOSIDASE FAMILY 31; 1.
DR Pfam; PF16874; Glyco_hydro_36C; 1.
DR Pfam; PF16875; Glyco_hydro_36N; 1.
DR Pfam; PF02065; Melibiase; 1.
DR PIRSF; PIRSF005536; Agal; 1.
DR PRINTS; PR00743; GLHYDRLASE36.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE 4: Predicted;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000262191}.
FT DOMAIN 26..280
FT /note="Glycosyl hydrolase family 36 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16875"
FT DOMAIN 653..783
FT /note="Glycosyl hydrolase family 36 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16874"
FT ACT_SITE 474
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT ACT_SITE 544
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT BINDING 195
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 362..363
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 439
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 472..476
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 522
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 544
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
SQ SEQUENCE 787 AA; 90625 MW; 1C7B324F090B8ED1 CRC64;
MIKKLENGFI LNTKNTTYAF RVLKTGHLEH LYYGKKIAIE NMEPLIEQHN FMPGNTVAYN
QETMEYSLEN MRLEMSSYGK GDVREPFVEV VKSDGSFTND FTYADSEISW GKQPFETLPG
SYDEDGNVDH LTVTLKDNDL VMELHYYVYE NCDVITRSTV LINKGKEKVN IKRLMSMQLD
MFGMNYVMTT FNGSWANEMN RHDTKVAAGR FANYSYAGTS SNRANPFVMI SREETTEDFG
ECIGTNLIYS GNHYEAMDVC SFNKTRFVSG INPQSMSYGL LPGEKFEAPE SVLTYSKKGY
NGMSQNMHKF VREHIVRGKW KYKERPVLLN SWEANYFDIT EEKLINLAKA GKDIGIELFV
MDDGWFGKRN DDTSSLGDWY VNKDKLPNGL AHLVDEINKI GLDFGIWIEP EMINKNSELY
KKHPNWVLEN PNAHHSEGRN QCMLDLTNNE VVDYMVKEIS NILSSANISY VKWDMNRIFS
DYYSAGLPYE SQGEVSHRYV LGFYKMAKAL TEKFPEILFE GCCGGGNRFD LGMLCYFPQI
WASDDTDAVC RVNIQNGYSY GYPMSTVGAH VSACPNHQTL RTTPIDTRYN VACFGVLGYE
CDLTKMSKED LFLIKKQIEE YKKWRKVLQF GEFYRGKSPY ENSQDSDSNN VYQWTCVSAD
KKKAVGMLFR KIIKPNDQFL NFRPKALAEN KEYHFYNRPV YHNKKDMGHF VYENNEIKSN
ENLSKDEVSF VAENFVKGET EDYIAYGSLL NEAGVNLKEG FAGIGVNGDT RIMKDFDSRL
YYMEEVE
//
