ID A0A376BVG7_9NEIS Unreviewed; 1224 AA.
AC A0A376BVG7;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=nitrate reductase (quinone) {ECO:0000256|ARBA:ARBA00012500};
DE EC=1.7.5.1 {ECO:0000256|ARBA:ARBA00012500};
GN Name=narG {ECO:0000313|EMBL:SSY80909.1};
GN ORFNames=NCTC10283_02473 {ECO:0000313|EMBL:SSY80909.1};
OS Alysiella crassa.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC Alysiella.
OX NCBI_TaxID=153491 {ECO:0000313|EMBL:SSY80909.1, ECO:0000313|Proteomes:UP000254209};
RN [1] {ECO:0000313|EMBL:SSY80909.1, ECO:0000313|Proteomes:UP000254209}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC10283 {ECO:0000313|EMBL:SSY80909.1,
RC ECO:0000313|Proteomes:UP000254209};
RG Pathogen Informatics;
RA Doyle S.;
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinol + nitrate = a quinone + H2O + nitrite;
CC Xref=Rhea:RHEA:56144, ChEBI:CHEBI:15377, ChEBI:CHEBI:16301,
CC ChEBI:CHEBI:17632, ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; EC=1.7.5.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001854};
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004202};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004202}. Membrane
CC {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004170}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. {ECO:0000256|ARBA:ARBA00010312}.
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DR EMBL; UFSO01000003; SSY80909.1; -; Genomic_DNA.
DR RefSeq; WP_034290685.1; NZ_UFSO01000003.1.
DR AlphaFoldDB; A0A376BVG7; -.
DR STRING; 1120980.GCA_000745955_00200; -.
DR OrthoDB; 9759518at2; -.
DR Proteomes; UP000254209; Unassembled WGS sequence.
DR GO; GO:0009325; C:nitrate reductase complex; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0008940; F:nitrate reductase activity; IEA:InterPro.
DR GO; GO:0045333; P:cellular respiration; IEA:UniProt.
DR GO; GO:0042126; P:nitrate metabolic process; IEA:InterPro.
DR CDD; cd02776; MopB_CT_Nitrate-R-NarG-like; 1.
DR CDD; cd02750; MopB_Nitrate-R-NarG-like; 1.
DR Gene3D; 3.40.50.12440; -; 1.
DR Gene3D; 4.10.1200.10; nitrate reductase tail; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR037943; MopB_CT_Nitrate-R-NarG-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR InterPro; IPR006655; Mopterin_OxRdtase_prok_CS.
DR InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR InterPro; IPR006468; NarG.
DR InterPro; IPR028189; Nitr_red_alph_N.
DR InterPro; IPR044906; Nitr_red_alph_N_sf.
DR NCBIfam; TIGR01580; narG; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR PANTHER; PTHR43105:SF2; RESPIRATORY NITRATE REDUCTASE 2 ALPHA CHAIN; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR Pfam; PF14710; Nitr_red_alph_N; 1.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
DR PROSITE; PS00932; MOLYBDOPTERIN_PROK_3; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000313|EMBL:SSY80909.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000254209}.
FT DOMAIN 43..107
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
SQ SEQUENCE 1224 AA; 138502 MW; 0601E17678AE7052 CRC64;
MSHFLDRLTF FKQKREPFSN GHGVLTTEDR GWEQAYRSRW QHDKIVRSTH GVNCTGSCSW
KVYVKNGLIT WETQQTDYPR TRPDLPNHEP RGCPRGASYS WYVYSAQRVK YPMMRGVLAE
MWRELRKTQS AIQAWETITT DPERAKKYKS QRGLGGFVRS TWDEAYEMIA AANAYTIKNY
GPDRVIGFSP IPAMSMVSYA AGARYLGLLG GVPLSFYDWY CDLPPASPQI WGEQTDVAES
ADWYNANYLM VWGSNVPLTR TPDAHFYTEV RYKGTKTVAV SSDFGEMAKF GDIWLAPKQG
TDAALAMAMG HVILKEFHVD KPSAYFTDYI RRLTDMPVLV RLQEKDGQYI PEYFLRASQL
DNHFNEEKNP DWKVLAIDER SGKLIVPNGS IGFRWDGSQK WNLETKAQGE EIQAALSLKN
LADDVVQVGF AYFGGEHDEL IHRKVPVKKV QLADGETALV ATVFDLMLAN YGVEHGLNDE
NCAQDYFDDK PYTPAWQEKH TGVQPEMVIR VAREFAQNAH DTEGRSMVIV GAGLNHWYHM
DMAYRGIINM LMMCGCIGKS GGGWCHYVGQ EKLRPQSGWT PLTFALDWHR PARQMNGTSF
FYAHTSQWRH EKLGVNEILA PTADGGLAKM SMIDYNAKAE RMGWLPSAPQ LSTNPLDLVA
QAESAGQDPV QYTVEKLKNG SLDMACNDPD NPQNFPRNLF VWRSNILGSS GKGHEYFLKY
LLGTQNAVMS DEEDCIKPTE IKVRPAVEGK LDLLTVLDFR MSTTCLYGDI VLPTATWYEK
DDLNTSDMHP FIHPLSEAVN PLWQSKTDWE IYKGLAKKFS ETAKDYLGVR KDIVLTPLMH
DSPQEMGQAF DPKDWKLGEC DPIPGKTMPM MTVVERDYGA IYEKFTSVGP LLEKINNNGK
GLTWDTKHEV EFLRKLNGVQ ATGAGQGQPK IETAIDAAEM ILTLAPETNG HVAMKAWQSL
GKATGRDHTH LIAASEHTQI RFRDIQAQPR KIVSSPIWSG VESEEVCYNA GYTNVHELIP
WRTITGRQQF YQDHKWMRDF GEGFCVYKPA VDFKTTAKLL GKHPNGNKEI TLNFLTPHQK
WGIHSTYSDN LRMLTLSRGG PHVWISEIDA KKAGIVDNDW VEVFNANGTI ACRVIVSQRI
PETMILMYHA QEKIVNTPAA EVSGKRGGIH NSVTRTVLKP THMIGGYAQQ AYGFNYYGTV
GSNRDEWVII RKMDKVEWKD EPVK
//
