ID A0A377KMD0_9ENTE Unreviewed; 743 AA.
AC A0A377KMD0;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 24-JAN-2024, entry version 28.
DE RecName: Full=Formate acetyltransferase {ECO:0000256|ARBA:ARBA00013897, ECO:0000256|RuleBase:RU368075};
DE EC=2.3.1.54 {ECO:0000256|ARBA:ARBA00013214, ECO:0000256|RuleBase:RU368075};
DE AltName: Full=Pyruvate formate-lyase {ECO:0000256|ARBA:ARBA00031063, ECO:0000256|RuleBase:RU368075};
GN Name=pflB {ECO:0000313|EMBL:STP29912.1};
GN ORFNames=EA71_01074 {ECO:0000313|EMBL:RCA10324.1}, GUJ97_00185
GN {ECO:0000313|EMBL:NAA79026.1}, NCTC8129_02143
GN {ECO:0000313|EMBL:STP29912.1};
OS Enterococcus durans.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=53345 {ECO:0000313|EMBL:STP29912.1, ECO:0000313|Proteomes:UP000254070};
RN [1] {ECO:0000313|EMBL:RCA10324.1, ECO:0000313|Proteomes:UP000252797}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=4EA1 {ECO:0000313|EMBL:RCA10324.1,
RC ECO:0000313|Proteomes:UP000252797};
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A.M., Van Tyne D., Lebreton F., Saavedra J.T., Gilmore M.S.,
RA Manson Mcguire A., Clock S., Crupain M., Rangan U., Young S.,
RA Abouelleil A., Cao P., Chapman S.B., Griggs A., Priest M., Shea T.,
RA Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Enterococcus durans 4EA1.";
RL Submitted (JUN-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:STP29912.1, ECO:0000313|Proteomes:UP000254070}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC8129 {ECO:0000313|EMBL:STP29912.1,
RC ECO:0000313|Proteomes:UP000254070};
RG Pathogen Informatics;
RA Doyle S.;
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:NAA79026.1, ECO:0000313|Proteomes:UP000444134}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BIOML-A60 {ECO:0000313|EMBL:NAA79026.1,
RC ECO:0000313|Proteomes:UP000444134};
RX PubMed=31477907; DOI=.1038/s41591-019-0559-3;
RA Poyet M., Groussin M., Gibbons S.M., Avila-Pacheco J., Jiang X.,
RA Kearney S.M., Perrotta A.R., Berdy B., Zhao S., Lieberman T.D.,
RA Swanson P.K., Smith M., Roesemann S., Alexander J.E., Rich S.A., Livny J.,
RA Vlamakis H., Clish C., Bullock K., Deik A., Scott J., Pierce K.A.,
RA Xavier R.J., Alm E.J.;
RT "A library of human gut bacterial isolates paired with longitudinal
RT multiomics data enables mechanistic microbiome research.";
RL Nat. Med. 25:1442-1452(2019).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + formate = CoA + pyruvate; Xref=Rhea:RHEA:11844,
CC ChEBI:CHEBI:15361, ChEBI:CHEBI:15740, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.1.54;
CC Evidence={ECO:0000256|ARBA:ARBA00001179,
CC ECO:0000256|RuleBase:RU368075};
CC -!- PATHWAY: Fermentation; pyruvate fermentation; formate from pyruvate:
CC step 1/1. {ECO:0000256|ARBA:ARBA00004809,
CC ECO:0000256|RuleBase:RU368075}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|RuleBase:RU368075}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|RuleBase:RU368075}.
CC -!- SIMILARITY: Belongs to the glycyl radical enzyme (GRE) family. PFL
CC subfamily. {ECO:0000256|ARBA:ARBA00008375,
CC ECO:0000256|RuleBase:RU368075}.
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DR EMBL; WXTH01000001; NAA79026.1; -; Genomic_DNA.
DR EMBL; LEPB01000004; RCA10324.1; -; Genomic_DNA.
DR EMBL; UGIF01000002; STP29912.1; -; Genomic_DNA.
DR RefSeq; WP_005878172.1; NZ_WXTW01000001.1.
DR STRING; 53345.LIU_06625; -.
DR GeneID; 56743713; -.
DR UniPathway; UPA00920; UER00891.
DR Proteomes; UP000252797; Unassembled WGS sequence.
DR Proteomes; UP000254070; Unassembled WGS sequence.
DR Proteomes; UP000444134; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008861; F:formate C-acetyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006006; P:glucose metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd01678; PFL1; 1.
DR Gene3D; 3.20.70.20; -; 1.
DR InterPro; IPR005949; Form_AcTrfase.
DR InterPro; IPR019777; Form_AcTrfase_GR_CS.
DR InterPro; IPR001150; Gly_radical.
DR InterPro; IPR004184; PFL_dom.
DR NCBIfam; TIGR01255; pyr_form_ly_1; 1.
DR PANTHER; PTHR30191; FORMATE ACETYLTRANSFERASE; 1.
DR PANTHER; PTHR30191:SF0; FORMATE ACETYLTRANSFERASE 1; 1.
DR Pfam; PF01228; Gly_radical; 1.
DR Pfam; PF02901; PFL-like; 1.
DR PIRSF; PIRSF000379; For_Ac_trans_1; 1.
DR SUPFAM; SSF51998; PFL-like glycyl radical enzymes; 1.
DR PROSITE; PS00850; GLY_RADICAL_1; 1.
DR PROSITE; PS51149; GLY_RADICAL_2; 1.
DR PROSITE; PS51554; PFL; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000256|RuleBase:RU368075};
KW Carbohydrate metabolism {ECO:0000256|RuleBase:RU368075};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|RuleBase:RU368075};
KW Glucose metabolism {ECO:0000256|ARBA:ARBA00022526,
KW ECO:0000256|RuleBase:RU368075};
KW Organic radical {ECO:0000256|ARBA:ARBA00022818,
KW ECO:0000256|PIRSR:PIRSR000379-2};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU368075}.
FT DOMAIN 1..613
FT /note="PFL"
FT /evidence="ECO:0000259|PROSITE:PS51554"
FT DOMAIN 620..743
FT /note="Glycine radical"
FT /evidence="ECO:0000259|PROSITE:PS51149"
FT REGION 603..625
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 407
FT /note="S-acetylcysteine intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000379-1"
FT ACT_SITE 408
FT /note="Cysteine radical intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000379-1"
FT MOD_RES 718
FT /note="Glycine radical"
FT /evidence="ECO:0000256|PIRSR:PIRSR000379-2,
FT ECO:0000256|PROSITE-ProRule:PRU00493"
SQ SEQUENCE 743 AA; 83795 MW; B5A2C0B607A9EC22 CRC64;
MEQWNGFKGT KWKEGVDTRD FIQNNYTEYR GDDSFLEKPT EATEKLWAEL QKLNDIQFER
NGVYDMDTEI VSTITSHDAG YLDKDLEKVV GLQTDKPLKQ AFMPFGGINM ANNALKSNGY
TPNDELTHIF TDWRKTHNQG VFDAYTAEMR AARKNKIITG LPDAYGRGRI IGDYRRIALY
GIDYLMEQKK KDHDMTGYHT MSEDVIRLRE EITEQYRALA QMKEMAAKYG YDISKPAANA
KEAVQWLYFG YLAAIKSQNG AAMSIGRVSA FLDIYIQRDL DNGVITEQEA QELIDHLVMK
LRMVKFARTP EYNQLFSGFP IWATLSIAGM GLDGRSLVTK NDFRLLHTLT NMGPSPEPNL
TVLYSEHLPE GFRTYASKIA IESSSIQFEN DNLLREQWGS DDCAIACCVS ATVVGKDMQF
FGARANLAKA LLYAINGGVD EVTGAQVGPE YRPISSETLD FEEVKHNFMN VMDWLAELYV
NTLNIIHYMH DKYSYEAAQL ALMDSELKRT FATGIAGISH AADSLAAIKY AKVKPIRNEK
GIAVDFEVEG DFPKYGNDDD NADKLAEWIL EYFMTQIKRH KTYRNSTPTT SLLTITSNVV
YGKNTGNTPD GREAGKPLAP GANPSYNAEQ NGLLASLNST AKINYAYARD GISNTQTINP
TGLGKDEDTR IGNLRNVLDG YFSKGAYHLN VNVFSNDLLR DAMENPMKYP NLTIRVSGYA
VKFRDLTREQ QLDVLMRTSH DRM
//
