ID A0A378AL31_KLEPO Unreviewed; 157 AA.
AC A0A378AL31;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 24-JAN-2024, entry version 18.
DE RecName: Full=Transcription elongation factor GreB {ECO:0000256|HAMAP-Rule:MF_00930};
DE AltName: Full=Transcript cleavage factor GreB {ECO:0000256|HAMAP-Rule:MF_00930};
GN Name=greB {ECO:0000256|HAMAP-Rule:MF_00930,
GN ECO:0000313|EMBL:STV12993.1};
GN ORFNames=NCTC5050_02660 {ECO:0000313|EMBL:STV12993.1};
OS Klebsiella pneumoniae subsp. ozaenae.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX NCBI_TaxID=574 {ECO:0000313|EMBL:STV12993.1, ECO:0000313|Proteomes:UP000255382};
RN [1] {ECO:0000313|EMBL:STV12993.1, ECO:0000313|Proteomes:UP000255382}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC5050 {ECO:0000313|EMBL:STV12993.1,
RC ECO:0000313|Proteomes:UP000255382};
RG Pathogen Informatics;
RA Doyle S.;
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Necessary for efficient RNA polymerase transcription
CC elongation past template-encoded arresting sites. The arresting sites
CC in DNA have the property of trapping a certain fraction of elongating
CC RNA polymerases that pass through, resulting in locked ternary
CC complexes. Cleavage of the nascent transcript by cleavage factors such
CC as GreA or GreB allows the resumption of elongation from the new
CC 3'terminus. GreB releases sequences of up to 9 nucleotides in length.
CC {ECO:0000256|HAMAP-Rule:MF_00930}.
CC -!- SIMILARITY: Belongs to the GreA/GreB family. GreB subfamily.
CC {ECO:0000256|HAMAP-Rule:MF_00930}.
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DR EMBL; UGLZ01000005; STV12993.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A378AL31; -.
DR Proteomes; UP000255382; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0070063; F:RNA polymerase binding; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0032784; P:regulation of DNA-templated transcription elongation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.10.50.30; Transcription elongation factor, GreA/GreB, C-terminal domain; 1.
DR Gene3D; 1.10.287.180; Transcription elongation factor, GreA/GreB, N-terminal domain; 1.
DR HAMAP; MF_00105; GreA_GreB; 1.
DR HAMAP; MF_00930; GreB; 1.
DR InterPro; IPR036953; GreA/GreB_C_sf.
DR InterPro; IPR018151; TF_GreA/GreB_CS.
DR InterPro; IPR028624; Tscrpt_elong_fac_GreA/B.
DR InterPro; IPR001437; Tscrpt_elong_fac_GreA/B_C.
DR InterPro; IPR023459; Tscrpt_elong_fac_GreA/B_fam.
DR InterPro; IPR022691; Tscrpt_elong_fac_GreA/B_N.
DR InterPro; IPR036805; Tscrpt_elong_fac_GreA/B_N_sf.
DR InterPro; IPR006358; Tscrpt_elong_fac_GreB.
DR NCBIfam; TIGR01461; greB; 1.
DR PANTHER; PTHR30437; TRANSCRIPTION ELONGATION FACTOR GREA; 1.
DR PANTHER; PTHR30437:SF6; TRANSCRIPTION ELONGATION FACTOR GREB; 1.
DR Pfam; PF01272; GreA_GreB; 1.
DR Pfam; PF03449; GreA_GreB_N; 1.
DR PIRSF; PIRSF006092; GreA_GreB; 1.
DR SUPFAM; SSF54534; FKBP-like; 1.
DR SUPFAM; SSF46557; GreA transcript cleavage protein, N-terminal domain; 1.
DR PROSITE; PS00830; GREAB_2; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00930}; Elongation factor {ECO:0000313|EMBL:STV12993.1};
KW Protein biosynthesis {ECO:0000313|EMBL:STV12993.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000255382};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_00930};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015, ECO:0000256|HAMAP-
KW Rule:MF_00930}.
FT DOMAIN 5..74
FT /note="Transcription elongation factor GreA/GreB N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF03449"
FT DOMAIN 81..155
FT /note="Transcription elongation factor GreA/GreB C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01272"
SQ SEQUENCE 157 AA; 18609 MW; 2760FAA2978CF4E4 CRC64;
MKTPLITREG YEKLKQEMDY LWRQERPEVT KKVTWAASLG DRSENADYQY NKKRLREIDR
RVRYLTKCLE QLKIVDYSPQ REGKVFFGAW VEIENDEGDI KRFRIVGYDE IFGRKDYISI
DSPMARALLK KEVGDLAIVN TPAGEASWYV NEIEYVK
//
