ID A0A380N0G6_9GAMM Unreviewed; 648 AA.
AC A0A380N0G6;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=protein-N(pi)-phosphohistidine--sucrose phosphotransferase {ECO:0000256|ARBA:ARBA00044053};
DE EC=2.7.1.211 {ECO:0000256|ARBA:ARBA00044053};
GN Name=bglF {ECO:0000313|EMBL:SUO98305.1};
GN ORFNames=NCTC10717_02047 {ECO:0000313|EMBL:SUO98305.1};
OS Suttonella indologenes.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Cardiobacteriales;
OC Cardiobacteriaceae; Suttonella.
OX NCBI_TaxID=13276 {ECO:0000313|EMBL:SUO98305.1, ECO:0000313|Proteomes:UP000254575};
RN [1] {ECO:0000313|EMBL:SUO98305.1, ECO:0000313|Proteomes:UP000254575}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC10717 {ECO:0000313|EMBL:SUO98305.1,
RC ECO:0000313|Proteomes:UP000254575};
RG Pathogen Informatics;
RA Doyle S.;
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N(pros)-phospho-L-histidyl-[protein](out) + sucrose = L-
CC histidyl-[protein] + sucrose 6(G)-phosphate(in);
CC Xref=Rhea:RHEA:49236, Rhea:RHEA-COMP:9745, Rhea:RHEA-COMP:9746,
CC ChEBI:CHEBI:17992, ChEBI:CHEBI:29979, ChEBI:CHEBI:64837,
CC ChEBI:CHEBI:91002; EC=2.7.1.211;
CC Evidence={ECO:0000256|ARBA:ARBA00043763};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
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DR EMBL; UHIA01000004; SUO98305.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A380N0G6; -.
DR OrthoDB; 92465at2; -.
DR Proteomes; UP000254575; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd00210; PTS_IIA_glc; 1.
DR CDD; cd00212; PTS_IIB_glc; 1.
DR Gene3D; 2.70.70.10; Glucose Permease (Domain IIA); 1.
DR Gene3D; 3.30.1360.60; Glucose permease domain IIB; 1.
DR InterPro; IPR011055; Dup_hybrid_motif.
DR InterPro; IPR036878; Glu_permease_IIB.
DR InterPro; IPR018113; PTrfase_EIIB_Cys.
DR InterPro; IPR001127; PTS_EIIA_1_perm.
DR InterPro; IPR003352; PTS_EIIC.
DR InterPro; IPR013013; PTS_EIIC_1.
DR InterPro; IPR001996; PTS_IIB_1.
DR InterPro; IPR010973; PTS_IIBC_sucr.
DR NCBIfam; TIGR00826; EIIB_glc; 1.
DR NCBIfam; TIGR00830; PTBA; 1.
DR NCBIfam; TIGR01996; PTS-II-BC-sucr; 1.
DR PANTHER; PTHR30175; PHOSPHOTRANSFERASE SYSTEM TRANSPORT PROTEIN; 1.
DR PANTHER; PTHR30175:SF4; PTS SYSTEM TREHALOSE-SPECIFIC EIIBC COMPONENT; 1.
DR Pfam; PF00358; PTS_EIIA_1; 1.
DR Pfam; PF00367; PTS_EIIB; 1.
DR Pfam; PF02378; PTS_EIIC; 1.
DR SUPFAM; SSF51261; Duplicated hybrid motif; 1.
DR SUPFAM; SSF55604; Glucose permease domain IIB; 1.
DR PROSITE; PS51093; PTS_EIIA_TYPE_1; 1.
DR PROSITE; PS00371; PTS_EIIA_TYPE_1_HIS; 1.
DR PROSITE; PS51098; PTS_EIIB_TYPE_1; 1.
DR PROSITE; PS01035; PTS_EIIB_TYPE_1_CYS; 1.
DR PROSITE; PS51103; PTS_EIIC_TYPE_1; 1.
PE 4: Predicted;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Phosphotransferase system {ECO:0000256|ARBA:ARBA00022683};
KW Reference proteome {ECO:0000313|Proteomes:UP000254575};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}.
FT TRANSMEM 113..134
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 154..175
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 182..199
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 261..285
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 346..368
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 403..424
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 445..466
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 5..88
FT /note="PTS EIIB type-1"
FT /evidence="ECO:0000259|PROSITE:PS51098"
FT DOMAIN 121..482
FT /note="PTS EIIC type-1"
FT /evidence="ECO:0000259|PROSITE:PS51103"
FT DOMAIN 526..630
FT /note="PTS EIIA type-1"
FT /evidence="ECO:0000259|PROSITE:PS51093"
FT ACT_SITE 27
FT /note="Phosphocysteine intermediate; for EIIB activity"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00421"
SQ SEQUENCE 648 AA; 68473 MW; BEBAED1B33C1BADA CRC64;
MSQYQHIAEQ IIAAVGGKDN ILAATHCATR LRMVLKDESL IDTAAIEAIE SAKGHFLTAG
QFQIIFGTGT VNNVTQALLQ LTGAQSVSTT EAKALGAEKG NIFQRFIKML SDIFVPIIPA
IVAGGLLMGL NNVFTANGLI VEGKSLIEVY PQWAALAEMI NTFANAPFVF LPILIAFSAT
KIFGGNPFLG AVLGMIMVHP DLTNNYALAE AIRTNTLDYW EFFGHQFAKV GYQGTVLPIL
VCSWILAKLE CSIRRVMPAS LDLLLTPLLS IFITAILTFS IVGPITRDAG IAFTDAVQWL
YNSSGAIGAA IFGAVYAPIV ITGMHNSFIP VETQLIADLA KSGGTFIFPI AAMNNVAQGA
AAFAALWISR DAKLKSIAGA SGISAVLGIT EPAMFGVNLR LRYPFYAALI GTALASAFIA
LFKVKAVALG AAGIPGIISI RADSIIPYVI GMAISFAATF TLTFVFSKQR RFQAAATNPA
PQTNQAEAVE TAVVEKVPAV AGSNPFPQEW QMPLAGEIIA LEQVPDPAFA SGAMGAGFAI
VPSGNQVISP VNGTIAAVFP SRHAIGIEAD NGLEILIHVG IDTVKLNGEG FTQHVQEGQA
VQAGELLLTV DFAAIEDKVP SIITPVVFTA MDKGEKIRIE NQRPILSE
//
