ID A0A383VTW3_TETOB Unreviewed; 650 AA.
AC A0A383VTW3;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 24-JAN-2024, entry version 18.
DE RecName: Full=assimilatory sulfite reductase (ferredoxin) {ECO:0000256|ARBA:ARBA00012353};
DE EC=1.8.7.1 {ECO:0000256|ARBA:ARBA00012353};
GN ORFNames=BQ4739_LOCUS8575 {ECO:0000313|EMBL:SZX68204.1};
OS Tetradesmus obliquus (Green alga) (Acutodesmus obliquus).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Sphaeropleales; Scenedesmaceae; Tetradesmus.
OX NCBI_TaxID=3088 {ECO:0000313|EMBL:SZX68204.1, ECO:0000313|Proteomes:UP000256970};
RN [1] {ECO:0000313|EMBL:SZX68204.1, ECO:0000313|Proteomes:UP000256970}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Cai Z.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-binding protein that binds to both double-stranded and
CC single-stranded DNA without significant sequence specificity to
CC reversibly repress the transcriptional activity of chloroplast
CC nucleoids by promoting DNA compaction and possibly regulate DNA
CC replication. {ECO:0000256|ARBA:ARBA00002010}.
CC -!- FUNCTION: Essential protein with sulfite reductase activity required in
CC assimilatory sulfate reduction pathway during both primary and
CC secondary metabolism and thus involved in development and growth.
CC {ECO:0000256|ARBA:ARBA00003329}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3 H2O + hydrogen sulfide + 6 oxidized [2Fe-2S]-[ferredoxin] =
CC 7 H(+) + 6 reduced [2Fe-2S]-[ferredoxin] + sulfite;
CC Xref=Rhea:RHEA:23132, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17359,
CC ChEBI:CHEBI:29919, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738; EC=1.8.7.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000993};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- COFACTOR:
CC Name=siroheme; Xref=ChEBI:CHEBI:60052;
CC Evidence={ECO:0000256|ARBA:ARBA00001929};
CC -!- SUBCELLULAR LOCATION: Plastid stroma {ECO:0000256|ARBA:ARBA00004646}.
CC Plastid, chloroplast stroma, chloroplast nucleoid
CC {ECO:0000256|ARBA:ARBA00004595}.
CC -!- SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S domain
CC family. {ECO:0000256|ARBA:ARBA00010429}.
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DR EMBL; FNXT01000845; SZX68204.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A383VTW3; -.
DR STRING; 3088.A0A383VTW3; -.
DR Proteomes; UP000256970; Unassembled WGS sequence.
DR GO; GO:0042644; C:chloroplast nucleoid; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0050311; F:sulfite reductase (ferredoxin) activity; IEA:UniProtKB-EC.
DR Gene3D; 3.30.413.10; Sulfite Reductase Hemoprotein, domain 1; 2.
DR Gene3D; 3.90.480.10; Sulfite Reductase Hemoprotein;Domain 2; 1.
DR InterPro; IPR005117; NiRdtase/SiRdtase_haem-b_fer.
DR InterPro; IPR036136; Nit/Sulf_reduc_fer-like_dom_sf.
DR InterPro; IPR006067; NO2/SO3_Rdtase_4Fe4S_dom.
DR InterPro; IPR045169; NO2/SO3_Rdtase_4Fe4S_prot.
DR InterPro; IPR045854; NO2/SO3_Rdtase_4Fe4S_sf.
DR InterPro; IPR006066; NO2/SO3_Rdtase_FeS/sirohaem_BS.
DR InterPro; IPR011787; SiR_ferredoxin-dep.
DR NCBIfam; TIGR02042; sir; 1.
DR PANTHER; PTHR11493:SF47; SULFITE REDUCTASE [NADPH] SUBUNIT BETA; 1.
DR PANTHER; PTHR11493; SULFITE REDUCTASE [NADPH] SUBUNIT BETA-RELATED; 1.
DR Pfam; PF01077; NIR_SIR; 1.
DR Pfam; PF03460; NIR_SIR_ferr; 2.
DR PRINTS; PR00397; SIROHAEM.
DR SUPFAM; SSF56014; Nitrite and sulphite reductase 4Fe-4S domain-like; 2.
DR SUPFAM; SSF55124; Nitrite/Sulfite reductase N-terminal domain-like; 2.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Heme {ECO:0000256|ARBA:ARBA00022617}; Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000256970};
KW Thioether bond {ECO:0000256|ARBA:ARBA00022784}.
FT DOMAIN 95..149
FT /note="Nitrite/Sulfite reductase ferredoxin-like"
FT /evidence="ECO:0000259|Pfam:PF03460"
FT DOMAIN 192..368
FT /note="Nitrite/sulphite reductase 4Fe-4S"
FT /evidence="ECO:0000259|Pfam:PF01077"
FT DOMAIN 387..452
FT /note="Nitrite/Sulfite reductase ferredoxin-like"
FT /evidence="ECO:0000259|Pfam:PF03460"
SQ SEQUENCE 650 AA; 72432 MW; D24A0236CF16C0B1 CRC64;
MQLLQQRTSA PSRQGLHVCN VAVPSKPPAF KSARRSKVEI IKEKSDYLRH PLMEELVNDN
PNINEEAMQL MKFHGSYMQD HREKRSFGLG KFYQFMMRTR QPSGLVTNQL YLVMDELADK
YGNGTLRLTT RQAYQLHGVL KHDLKTVFST VIKNMGSTLG ACGDVNRNVM GPAAPFVNKP
EYMLAQKYAN DIADLLAPQS GAYYDVWLDG EKFVSVYKED PKVTADRAFN GYGTNYTNSP
EPIYGTQFLP RKFKVAVTVP GDNSVDIFTN DIGVVVMTDA NGELQGFNLL VGGGMGRTHR
SNETFPRLAD PIGYVDKDDI FHAIKAIVAT QRDYGRRDDR KQARLKYLVD EWGVDKFRSV
VEQYMGKRFQ DFKPLPAWKF EDYLGWHEQG DGKLFYGVYV QNGRLKGDAK VALRGVIERY
ELPVTLTANQ NIILRDVQPA WKEDIQATLE AAGLTDVADL SGVDRLSMAC PALPLCGLAI
TEAERGLPDV NKRVLALLNR LGLGDEPITM RMTGCPNGCA RPYMAEIGYV GDGPNSYQLW
LGGSPNQTRL AQVFAERVKL RDLESTLEPL LAAFKARRQP GEAFGDFVAR LGFAALRGYS
ESYVPAHKVD SLPTVSIDED TFKALEAVAA AEGKTIAHVA SEKLQKLNGQ
//
