ID A0A384A979_BALAS Unreviewed; 455 AA.
AC A0A384A979;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 08-NOV-2023, entry version 19.
DE RecName: Full=Bleomycin hydrolase {ECO:0000256|ARBA:ARBA00022227, ECO:0000256|PIRNR:PIRNR005700};
DE EC=3.4.22.40 {ECO:0000256|ARBA:ARBA00012465, ECO:0000256|PIRNR:PIRNR005700};
GN Name=BLMH {ECO:0000313|RefSeq:XP_007183900.1};
OS Balaenoptera acutorostrata scammoni (North Pacific minke whale)
OS (Balaenoptera davidsoni).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Whippomorpha; Cetacea; Mysticeti;
OC Balaenopteridae; Balaenoptera.
OX NCBI_TaxID=310752 {ECO:0000313|Proteomes:UP000261681, ECO:0000313|RefSeq:XP_007183900.1};
RN [1] {ECO:0000313|RefSeq:XP_007183900.1}
RP IDENTIFICATION.
RC TISSUE=Muscle {ECO:0000313|RefSeq:XP_007183900.1};
RG RefSeq;
RL Submitted (JAN-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by
CC hydrolysis of a carboxyamide bond of beta-aminoalanine, but also
CC shows general aminopeptidase activity. The specificity varies
CC somewhat with source, but amino acid arylamides of Met, Leu and Ala
CC are preferred.; EC=3.4.22.40;
CC Evidence={ECO:0000256|ARBA:ARBA00000423,
CC ECO:0000256|PIRNR:PIRNR005700};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR005700}.
CC -!- SIMILARITY: Belongs to the peptidase C1 family.
CC {ECO:0000256|PIRNR:PIRNR005700}.
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DR RefSeq; XP_007183900.1; XM_007183838.1.
DR AlphaFoldDB; A0A384A979; -.
DR GeneID; 103008350; -.
DR CTD; 642; -.
DR OrthoDB; 45184at2759; -.
DR Proteomes; UP000261681; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0070005; F:cysteine-type aminopeptidase activity; IEA:InterPro.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd00585; Peptidase_C1B; 1.
DR Gene3D; 3.90.70.10; Cysteine proteinases; 1.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR000169; Pept_cys_AS.
DR InterPro; IPR004134; Peptidase_C1B.
DR PANTHER; PTHR10363; BLEOMYCIN HYDROLASE; 1.
DR PANTHER; PTHR10363:SF2; BLEOMYCIN HYDROLASE; 1.
DR Pfam; PF03051; Peptidase_C1_2; 1.
DR PIRSF; PIRSF005700; PepC; 1.
DR SUPFAM; SSF54001; Cysteine proteinases; 1.
DR PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|PIRNR:PIRNR005700};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005700};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PIRNR:PIRNR005700};
KW Reference proteome {ECO:0000313|Proteomes:UP000261681};
KW Thiol protease {ECO:0000256|ARBA:ARBA00022807,
KW ECO:0000256|PIRNR:PIRNR005700}.
FT ACT_SITE 73
FT /evidence="ECO:0000256|PIRSR:PIRSR005700-1"
FT ACT_SITE 372
FT /evidence="ECO:0000256|PIRSR:PIRSR005700-1"
FT ACT_SITE 396
FT /evidence="ECO:0000256|PIRSR:PIRSR005700-1"
SQ SEQUENCE 455 AA; 52300 MW; 8E508E04E5DB6389 CRC64;
MNDAGLNSEK VAALLQKLNS DPQFVLAQNV GTTHDLLDIC LKRATVQGVQ HVFQHTVHPE
GKPVTSQKSS GRCWIFSCLN VMRLPFMRKL NIEEFEFSQS YLFFWDKVER CYFFLNAFVD
TAQKKEPEDG RLVQYLLTNP ANDGGQWDML VNIVEKYGVI PKKCFPESYT TEATKRMNDI
LNHKMREFCL RLRNLVHSGA TKGEISATQD AMMEEIFRVV CICLGNPPET FTWEYRDKDK
NYQKIGPITP LKFYKEHVKP LFNMEDKICL VNDPRPHHKY SRLYTVDYLS NMVGGRKTLY
NNQPIDLLKK VVAASIKDGE AVWFGCDVGK HFNGKLGLSD MNVYDHELVF GVSMKNMNKA
ERLTFGESLM THAMTFTAVS EKDDQEGAFV KWRVENSWGE DHGHKGYLCM TDEWFSEYVY
EVVVDRKHIP EGVLAVLEQE PIVLPAWDPM GALAE
//
