ID   A0A387B8Z8_9MICO        Unreviewed;       860 AA.
AC   A0A387B8Z8;
DT   05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT   05-DEC-2018, sequence version 1.
DT   24-JAN-2024, entry version 20.
DE   RecName: Full=Arabinogalactan endo-beta-1,4-galactanase {ECO:0000256|RuleBase:RU361192};
DE            EC=3.2.1.89 {ECO:0000256|RuleBase:RU361192};
GN   ORFNames=D7I47_04865 {ECO:0000313|EMBL:AYF97655.1};
OS   Protaetiibacter intestinalis.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC   Protaetiibacter.
OX   NCBI_TaxID=2419774 {ECO:0000313|EMBL:AYF97655.1, ECO:0000313|Proteomes:UP000278886};
RN   [1] {ECO:0000313|Proteomes:UP000278886}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=2DFWR-13 {ECO:0000313|Proteomes:UP000278886};
RA   Heo J., Kim S.-J., Kwon S.-W.;
RT   "Genome sequencing of strain 2DFWR-13.";
RL   Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic
CC         linkages in type I arabinogalactans.; EC=3.2.1.89;
CC         Evidence={ECO:0000256|RuleBase:RU361192};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 53 family.
CC       {ECO:0000256|ARBA:ARBA00010687, ECO:0000256|RuleBase:RU361192}.
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DR   EMBL; CP032630; AYF97655.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A387B8Z8; -.
DR   KEGG; lyd:D7I47_04865; -.
DR   OrthoDB; 3981930at2; -.
DR   Proteomes; UP000278886; Chromosome.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031218; F:arabinogalactan endo-1,4-beta-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0015926; F:glucosidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   Gene3D; 1.20.1270.90; AF1782-like; 1.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR011081; Big_4.
DR   InterPro; IPR011683; Glyco_hydro_53.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR001995; Peptidase_A2_cat.
DR   PANTHER; PTHR34983; ARABINOGALACTAN ENDO-BETA-1,4-GALACTANASE A; 1.
DR   PANTHER; PTHR34983:SF2; ENDO-BETA-1,4-GALACTANASE; 1.
DR   Pfam; PF07532; Big_4; 1.
DR   Pfam; PF07745; Glyco_hydro_53; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   PROSITE; PS50175; ASP_PROT_RETROV; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|RuleBase:RU361192};
KW   Hydrolase {ECO:0000256|RuleBase:RU361192};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000278886};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        835..854
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          820..834
FT                   /note="Peptidase A2"
FT                   /evidence="ECO:0000259|PROSITE:PS50175"
FT   REGION          717..737
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   860 AA;  89666 MW;  4FE2E840CDB1DC98 CRC64;
     MHAHSIRTGL GIGAVTAVAL ALVGIPSAIA AADGPVEAGI FVDRIDGLGD DFINGVDVSS
     ILSEEESGVV YYDTEGHQAD LFDVLEDADI NYVRVRVWDD PYDAEGNGYG GGTTDLARAV
     EIGERATAHG MRVLVDFHYS DFWADPAKQH VPKAWEGYTT EQLVTALGAY TEASLDAFAD
     AGVDVGMVQI GNETNNTIAG VADWDGKAAM FSAGSAAVRE VFPDALVALH FTNPETPNRY
     TGYAAQLAAH DVDYDVFASS YYNFWHGSPE NLTSVLSTIA DSYDKKVMVA ETSYVYTFDD
     GDGWQNSVNA GNFSGDYPAT VQGQADLIHD VMAAVAAVGD AGLGVFYWEP AWIPVGTPDQ
     LAANQQKWEQ YGSGWASSYA GSYDPVDAGV YYGGSSWDNQ ALFDFTGHPL ESLQTFRYAR
     TGAVTERVAT SIETVALTVY DGSPIALPAT IEVGYNDGST EHPAVAWSDA VDWIRGPGVY
     SIPGTIASGE AITATVTVLQ ANYVVNHGFE DADTSMWSLA TNPSGAASIT TTSNTNEGTK
     ALDFWAASAF TIQLQQTVTG VPAGEYTLQA TAQGGALGDS DTLTLWAYTD DDSADTWMWL
     YGWRDYRTFT TPPLTVGDDG TVTVGAYISA TGGAWGTLDD LRLVRYIPDA ALDTGALEEL
     LDEADGVDRA LYTEGSLGGL DDAVASAQVV LAGSGDQDDV DAVVALLRAA LDGLALAPSG
     EPSGEPSGEP SEGPVDAPVI TLGAGTVTPG GTLHVTVSGL EEGSIEIGVA STYRALATAS
     VVAGTASATV TIPADLEAGL HHIQVRDGSG ALLAEAELRV LALGDTGADE TVIRVLAALG
     AVLVLGGAAA FAWGRRRAAA
//