ID A0A388KGG8_CHABU Unreviewed; 4365 AA.
AC A0A388KGG8;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=RNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012493};
DE EC=2.7.7.49 {ECO:0000256|ARBA:ARBA00012493};
GN ORFNames=CBR_g3864 {ECO:0000313|EMBL:GBG69164.1};
OS Chara braunii (Braun's stonewort).
OC Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae;
OC Chara.
OX NCBI_TaxID=69332 {ECO:0000313|EMBL:GBG69164.1, ECO:0000313|Proteomes:UP000265515};
RN [1] {ECO:0000313|EMBL:GBG69164.1, ECO:0000313|Proteomes:UP000265515}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S276 {ECO:0000313|EMBL:GBG69164.1,
RC ECO:0000313|Proteomes:UP000265515};
RX PubMed=30007417; DOI=10.1016/j.cell.2018.06.033;
RA Nishiyama T., Sakayama H., Vries J.D., Buschmann H., Saint-Marcoux D.,
RA Ullrich K.K., Haas F.B., Vanderstraeten L., Becker D., Lang D.,
RA Vosolsobe S., Rombauts S., Wilhelmsson P.K.I., Janitza P., Kern R.,
RA Heyl A., Rumpler F., Villalobos L.I.A.C., Clay J.M., Skokan R., Toyoda A.,
RA Suzuki Y., Kagoshima H., Schijlen E., Tajeshwar N., Catarino B.,
RA Hetherington A.J., Saltykova A., Bonnot C., Breuninger H., Symeonidi A.,
RA Radhakrishnan G.V., Van Nieuwerburgh F., Deforce D., Chang C., Karol K.G.,
RA Hedrich R., Ulvskov P., Glockner G., Delwiche C.F., Petrasek J.,
RA Van de Peer Y., Friml J., Beilby M., Dolan L., Kohara Y., Sugano S.,
RA Fujiyama A., Delaux P.-M., Quint M., TheiBen G., Hagemann M., Harholt J.,
RA Dunand C., Zachgo S., Langdale J., Maumus F., Straeten D.V.D., Gould S.B.,
RA Rensing S.A.;
RT "The Chara Genome: Secondary Complexity and Implications for Plant
RT Terrestrialization.";
RL Cell 174:448-464(2018).
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GBG69164.1}.
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DR EMBL; BFEA01000110; GBG69164.1; -; Genomic_DNA.
DR EnsemblPlants; GBG69164; GBG69164; CBR_g3864.
DR Gramene; GBG69164; GBG69164; CBR_g3864.
DR Proteomes; UP000265515; Unassembled WGS sequence.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR CDD; cd00303; retropepsin_like; 1.
DR CDD; cd09274; RNase_HI_RT_Ty3; 2.
DR CDD; cd01647; RT_LTR; 2.
DR Gene3D; 1.10.340.70; -; 2.
DR Gene3D; 3.30.70.270; -; 4.
DR Gene3D; 2.40.70.10; Acid Proteases; 1.
DR Gene3D; 3.10.10.10; HIV Type 1 Reverse Transcriptase, subunit A, domain 1; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR Gene3D; 1.25.40.10; Tetratricopeptide repeat domain; 4.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR001584; Integrase_cat-core.
DR InterPro; IPR041588; Integrase_H2C2.
DR InterPro; IPR021109; Peptidase_aspartic_dom_sf.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR000477; RT_dom.
DR InterPro; IPR041373; RT_RNaseH.
DR InterPro; IPR041577; RT_RNaseH_2.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR PANTHER; PTHR37984:SF7; INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR37984; PROTEIN CBG26694; 1.
DR Pfam; PF17921; Integrase_H2C2; 2.
DR Pfam; PF17917; RT_RNaseH; 1.
DR Pfam; PF17919; RT_RNaseH_2; 1.
DR Pfam; PF00665; rve; 1.
DR Pfam; PF00078; RVT_1; 2.
DR Pfam; PF13181; TPR_8; 1.
DR SMART; SM00028; TPR; 7.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 3.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR SUPFAM; SSF48452; TPR-like; 3.
DR PROSITE; PS50994; INTEGRASE; 1.
DR PROSITE; PS50878; RT_POL; 1.
DR PROSITE; PS50005; TPR; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000265515};
KW TPR repeat {ECO:0000256|PROSITE-ProRule:PRU00339}.
FT DOMAIN 1013..1192
FT /note="Reverse transcriptase"
FT /evidence="ECO:0000259|PROSITE:PS50878"
FT DOMAIN 1961..2130
FT /note="Integrase catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50994"
FT REPEAT 3319..3352
FT /note="TPR"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00339"
FT REGION 417..453
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 668..694
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2532..2551
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4318..4339
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4365 AA; 489407 MW; 2EA848070EDA1D0E CRC64;
MLFGCELIPF VSSSGSGAEL HPATGDKAKV RGGGEVILFS ANKRGGGGGR GGEGLNGYGL
CYVYSELNLI ITLFLLQGLA ELHTATGDKA KVIDAWEHIR QLSSEAGDTD RVYDYTRRLA
LAYGTEGRRE DELKTWRSLA NSNEGAVSSA VRIEALCRIA DAEMETMTQD IDAAAKRGIA
KWKKANQQLA SAELGGPVTD SQDSWNEDKV RRLVADEWRD AHAGVVESTL LDVVEAAPAF
GEQLHATSAV PRQSQCHVSM TGPAMGLPGQ LANEPIANYK KRFQAQLAFI EAEEQRQLAA
AAARLQAEEA ATAEKLRLQA EADVDAQAPR KEAQDLLQRH EANSIERLEF WHFEPNGDDA
TPEEQRKEFL SKLVTRLLCA CNYQRSELEK QNQELQQQYQ DLKTQHQELA NLRRMVQSHE
DATRAPNSRV LDLEQAVPGP NAGASSSAPS SRQLEERVDH IVAMLGDIST FAAPTTISNQ
LDTLKTEFQQ LQSTNTDGNN PKQYKMPIFQ LEKFDDYTHQ DPVLWWEAFT TQLRILPVAK
HAYIGALFMN SKGGCEIWLT HLAATHSVDV ADLKDKIAWE ELTRLWKKRF IVDDAPALAI
NRLFTMSQGN TATREATPPL TPPDLAALLA ASYTSGEDAH VASPRYTYED YAVHLVPPLD
QPLHVQXSTX CTVSSPSTTD STASPSSTAG DSTSWSRLEE LDPLTFEDFQ WMPLPRSGRL
PKPHCNVLKA QLRDYLHTAV QTLLMDARVE VVDLHAYIAK IDREFKTQRY DDIDAPLLYV
RIQIGEATCS ALIDCRASRN YMSQDFMVRA GLGPRVRRKA HPTQVTLADG HTHKSIDRCI
DDVPVYFAPH ASEAVSFDIL DTKFDMILGM SWLRSKDHPV NFYRRTVHVR DRNGVVVPCT
VAPPHPSVSC HVVSAASMRA SIIRDDIEEM GVCFLHALPP QDASSMDSSS DPHITELLDA
YSDVFEGPHG VVPDRPIRHE IILEDGAVPP RGCIYRMSEE ELSVLRAQLD DLQEKGRIRP
SSSPYDAIVL FVRKKNKDLR LCIDYRKLNA QTIRNVGPLP RIDDLLERLG GAKFFSKLDL
KSGYHQLEIR KEDRYKTAFK TRYGHFEWLV MPFGLTNAPT TFQAAMATEF RHILDRYVLI
YLDDILVYSR SLEEHVEHLR TVLERLRQAK YKANRDKCEF ARQELEYLGH YVTPQGIRPL
ADKIEALRVW PEPTNTTDVR AFMGLAGYYQ RFITGYSRIA APMTRLQSPK VPFVFDDDAC
RSFQALKTAM LMAPVLNIYD PTLPTRVRTA ASLVLEQCVR MIKKQSAIST PWEVAMSFCE
DDHANEICEP LRPIFDSTGM NLEQEWSQMR RNVWKGLLGR KLLHAFPLHY DGLVGLVCAL
RTEISRSIRQ GAGVFEEALI QYQENCSSVS GWLMLTELKL LHQDFEGTLE CADKGRQCVI
QAQDELGLGL SRAEMELIVL QAKALAELGD LDRAESLLHH VLQRAEAASL DDKLHIGMAA
KKQAQIVTDG LLKLYSRKYK SVRELTTTVE RLIVVPGVEY NPQVLLTMFL RCLPTEIKNL
LASDACLEYH TFETFSKKAL DLEAMLGGAQ TPATDERKKK TPQEWKKKGS RLMMVDSDGN
QIEIDDVSEL VEVSKLDGKE SVEGSNLAAV VKTKAGGRGK GGQQRSQGQV ANPNKIAAWV
RAGLDQEVWR DRWSRGACIN YDEYGHQQFK CKNPKSRRRS LLREGKKLRP VEHMSKKMPS
KKLAKSTYER ELYALYKALV HWRHYPLGRC FYLRTDHHTL KWIKTQPVLS DALKRWTAFI
DQYDFKLDYV KGEYNKVVDA LSRRENYLCA LISEFGLSED VTRSLGETYK EDPLTMDIIN
KQQAKDKATS DEFVMVDGLE KARFKRLVVP SREILRSSFL GECHDATGHF GYKKTCANLV
QRFWWPNMLD DAKKYVQTSQ VCPRDKPRTQ APLGLLKPLP IPAGPGQSIS MDFMDTLVTS
KNGKRHIFVI VDRFTKYTRV IAMLETAGTE HVVKLFMDNW VRDFGLPKTI VSDRDVRFTS
EMWKKAAEQM GSQLQMTSGN HPEANGQGEQ MNQVVQHLLR HYIKPSQDDW DEKLPLIASL
YNNAVHSSTD VSLNQLHLGW KPRSALDVLL PENRTAATPG TIEFGVQYEK LLQQTVEHIK
KSQEAMIASE NKRRRQSIFQ GLAGVCLRSG KDAEGRQLLE EIVEWDDRND WAVGELGWLA
FTNGDNQAAI DLLSSAISIQ PKSYLHHLRL GKVYWNSREE LTDGSSKSHA RLLEAAKINP
RCAEAFRYLG LVYRTALGDL QRASRCFQKA ISLDAQDRVS GISSATSAVP GHIAPSDIVP
RQQHHVRHSA TPAVTSDTAP RQQCHVSNMT GLPGQLANET IAAYKQRCLA QIEAEEQRLL
AVEAARIKAE EAAAAEKLRL QADADADSQA RRKEAQDLLQ RHEATSIDKL KFWLFKPNGD
EATPEEQHKE FLSKLVTRLL YACNYQRSEL ERQHQDLTQQ HQELATLRRT VQCHEDATRA
LNARLLNLEQ TVPGPAAGAS SSAPPSRQLE DRVDHVVAML GDISTFAAPT TTISSQLHTL
KTEVQQLQTT NANGNPKMYK MPTFTLEKFD DYTQQDPVLW WEAFTTQLRI LPGGCQTWLT
HLATSHGVDV PDLKDVITWE ELTRLWKKRF IIDDAPALAI NRLFTMSQGN TATRDWLTEW
QKIAAAPNLN LPFEHLRREF YNRSSAALSQ ALGDREQYAT FVEIIDKARE IIKTNRTVSM
GQVWLDRGGV QSSRPLRQLL LVQQHQAQDL PVPGSGQGGC ATPPQLGKLS FAEGYHQLEI
RKEDRYKTAF KTRYGHFEWL VMPFGLTNAP AIFQAAMTTE FRHMLDRYVL IYLDDILVYS
RSLEEHVEHP RTVLEWLRQA KYKANHDKCE FARQELEYLG HYVTPQGIRP LADKIEALRV
WPEPTNTTDL RSFMGLAGYY QRFITGYSRI AAPMTRLQSP KVPFLFDDDA RQSFQALKTA
MLMAPVLSIY DPTLPTRVTT DASGYGIGAV LEQHDGDDWH PVEYFSHKVP PINLLDDARK
KELLAFVMAL KRRRHFLLGR RRFTWVTDNN PLTYYKTQDT VSSTIGRWMY FIDQFDFTPK
HVPDLSNRAA DALSRRPDLC AMTHHAFAFD EELQRQFIRA YESDPDFGTL YAQLSSDHPP
ASHYRIADQY LLLHSRGKDL LCVPRDRRLR TRLLGEYHDS RLAGHFGVNR TIARLRQRFR
WPDLITDVTR YCDSCKVCRR SKPRNRNPYG GLHPMPIPRE PGLSIAMDVT GPFPRDRLGH
DGILTVVDRL SLLRWHADEA LPLALAYAYQ QLGRFTAALK SYGRVLQLDS DRVFSLIQAS
IINHQMGLHS KAVEGFQRAL CRSPKHVAAL CGLVSALKGQ AEVSVFSGAY GAAASFLKEA
ESHIVLCREL HPTLQLARKL HGDIMILYAR VVPYEDDTKD EKGAEDDKAV EESKGAVGIL
KKMEEEKKEA GLRAIRSYSH ALHKHPSNEG AWADLAFAYY QHAHLLSLQL VPKAPKEEIE
RLSSFAERLA LGALRLNGGN AMLWNLLGVV ARRKFLRQHA FIRALQIDSK LPIVWANLGQ
MPEARWGFGL VASRAGLLHK AEVYAALQQC ITQTPQEVSV FNVAALSAEA RGLIQEAIAL
LKAAEEIACL EVGSPMASEE LRKDQVSSLQ KKIMISLNLA RVLMKAGRAA EALAIYQHVL
HDEVASAASA SLLRGQNELS IIRGYAIALW SSGHDSSVAT STGIQSLTTA LQKASSSPPS
PQYLATLRVL CQLYYHHLGP SGTAAILDCL TAVPAVCLED TQLQSAGLAS VVASRRFIDM
SHMLSLCRTL FDHSSAHVGQ MIITDAYCIQ GDYAQAVRHL KRALHRYPHL VSLRGALGRA
LLNLSIENVP KALHICLLSD EGFSDAENRE GKALCDAVCA WCAFACYACG MARGCHSTPF
DHHTQAEQLQ TLCRKLQRLV HMNPSNGSAR LLMIATGLQR ARSLHYPSGL CTGLLRASMQ
SLSLSVKEGL SPLERALLAI CASELALHVR KDGAISLGLR LAKKAVQVAN VPDSKPESPL
HAVSPLVRRQ IGRCHAAAGE WHQAFLAHQE AMSDALERFD FPAALEVLEA MYLVGKKSEA
EVELRRFEQA VQQMGTVGEK NVWLGALRAL QGQTKGDEGD GPAAVRFTSE AVTWTDEDNA
VLHYLDGALR LESAEAEKDI EGVVLAKRSL GRALIMTQSE LGKPKVSPLS HAGVGAGIGG
IYLLLARAEM LSNRGAPPSA SRLAKWTDLI KHEWSLWPRG TQPAELCFQM GVISGMSSSS
KGGDSWEEEG SNRNKTPSLG TQGWFQRAVH LDPTCHRFWR RMILA
//
