ID A0A388KLN4_CHABU Unreviewed; 3957 AA.
AC A0A388KLN4;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 24-JAN-2024, entry version 18.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU363044};
DE EC=3.6.4.12 {ECO:0000256|RuleBase:RU363044};
GN ORFNames=CBR_g8252 {ECO:0000313|EMBL:GBG70952.1};
OS Chara braunii (Braun's stonewort).
OC Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae;
OC Chara.
OX NCBI_TaxID=69332 {ECO:0000313|EMBL:GBG70952.1, ECO:0000313|Proteomes:UP000265515};
RN [1] {ECO:0000313|EMBL:GBG70952.1, ECO:0000313|Proteomes:UP000265515}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S276 {ECO:0000313|EMBL:GBG70952.1,
RC ECO:0000313|Proteomes:UP000265515};
RX PubMed=30007417; DOI=10.1016/j.cell.2018.06.033;
RA Nishiyama T., Sakayama H., Vries J.D., Buschmann H., Saint-Marcoux D.,
RA Ullrich K.K., Haas F.B., Vanderstraeten L., Becker D., Lang D.,
RA Vosolsobe S., Rombauts S., Wilhelmsson P.K.I., Janitza P., Kern R.,
RA Heyl A., Rumpler F., Villalobos L.I.A.C., Clay J.M., Skokan R., Toyoda A.,
RA Suzuki Y., Kagoshima H., Schijlen E., Tajeshwar N., Catarino B.,
RA Hetherington A.J., Saltykova A., Bonnot C., Breuninger H., Symeonidi A.,
RA Radhakrishnan G.V., Van Nieuwerburgh F., Deforce D., Chang C., Karol K.G.,
RA Hedrich R., Ulvskov P., Glockner G., Delwiche C.F., Petrasek J.,
RA Van de Peer Y., Friml J., Beilby M., Dolan L., Kohara Y., Sugano S.,
RA Fujiyama A., Delaux P.-M., Quint M., TheiBen G., Hagemann M., Harholt J.,
RA Dunand C., Zachgo S., Langdale J., Maumus F., Straeten D.V.D., Gould S.B.,
RA Rensing S.A.;
RT "The Chara Genome: Secondary Complexity and Implications for Plant
RT Terrestrialization.";
RL Cell 174:448-464(2018).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- SIMILARITY: Belongs to the helicase family.
CC {ECO:0000256|RuleBase:RU363044}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GBG70952.1}.
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DR EMBL; BFEA01000138; GBG70952.1; -; Genomic_DNA.
DR STRING; 69332.A0A388KLN4; -.
DR EnsemblPlants; GBG70952; GBG70952; CBR_g8252.
DR Gramene; GBG70952; GBG70952; CBR_g8252.
DR Proteomes; UP000265515; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR CDD; cd18809; SF1_C_RecD; 1.
DR CDD; cd18795; SF2_C_Ski2; 1.
DR Gene3D; 1.10.3380.30; -; 2.
DR Gene3D; 1.20.1500.20; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR010285; DNA_helicase_pif1-like.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR025476; Helitron_helicase-like.
DR InterPro; IPR025696; MTR4_beta-barrel.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR049163; Pif1-like_2B_dom.
DR InterPro; IPR012961; Ski2/MTR4_C.
DR InterPro; IPR040801; Ski2_N.
DR PANTHER; PTHR12131; ATP-DEPENDENT RNA AND DNA HELICASE; 1.
DR PANTHER; PTHR12131:SF24; DEXH-BOX ATP-DEPENDENT RNA HELICASE DEXH11; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF08148; DSHCT; 1.
DR Pfam; PF14214; Helitron_like_N; 1.
DR Pfam; PF13234; MTR4_beta-barrel; 1.
DR Pfam; PF05970; PIF1; 1.
DR Pfam; PF21530; Pif1_2B_dom; 1.
DR Pfam; PF17911; Ski2_N; 1.
DR SMART; SM00382; AAA; 2.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM01142; DSHCT; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 3.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363044};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA damage {ECO:0000256|RuleBase:RU363044};
KW DNA recombination {ECO:0000256|RuleBase:RU363044};
KW DNA repair {ECO:0000256|RuleBase:RU363044};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|RuleBase:RU363044};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU363044};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363044};
KW Reference proteome {ECO:0000313|Proteomes:UP000265515}.
FT DOMAIN 2967..3122
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 3228..3430
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 1..317
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 516..617
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1681..1709
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1737..1756
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1785..1837
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1878..1909
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1941..1972
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2434..2497
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2698..2780
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2802..2837
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2867..2891
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3170..3201
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3576..3596
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 2395..2422
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 1..24
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 25..47
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 48..68
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 103..137
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 138..153
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 212..307
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 546..560
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 579..617
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1878..1895
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1956..1972
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2438..2490
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2760..2780
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2810..2825
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3957 AA; 436565 MW; A609EC4FD2DCE248 CRC64;
MMGRRRREWS RDWRDNEVEE KRARRRRRRT TMKKKRARRR TKKRWRRRRE VVAMEKNEED
DGEGGGRGGG GDLDDGGGGG GGRRGGGGGG GGGGEGGRRK GGGGGGRAKR ARRRRGRRRR
MTKKKRARRW TKKRWRRRRE VAAMEKNEED DGEGGGGGGG GLDDGRGGGE RRGGKGGRRG
GGGRRGGGGE RRGGGGGGEG GRRRGGGGGG GHEEEEEEEE EEEEEEEEEE EEADNEEGEE
EEEEEEVEEK EDDEEEEEEE EEEATVNEED EEEEEEEDEE EEEEEEEEEE EEEEEEEEEE
EEEEGPTMEE LAHLAWAGNG SWEGRNLLPR NERCAPFAVV TIAEKNTLSS DSAVRHSSIR
LGENECGVPT GATTHWRGGK VLDTGHSLPR GHASFVEERG REWALNEDLG RTQGQRAALS
EASSRSYDFG ACHGWLDGGS LGGVVPVPCS VSWPGTDNVA FESGEAGGVA PDVDSTVFGD
GRVFQLPLEM EQEPSCVPQC VRAACCAAGS LDGLDPQGKS VPVEGGVGGG SCEPVRKDRA
SDGPVSGDNS GRSVGQGLAS SMATARAARR LRVSSRRKRR ASTPGEGAQH RSDLERHAPA
IEEGGEGAEE RHVDTGSRSG VDVAMLRFGS VSLASDAVAD YPTLPKATPT TSPNNFMRSI
CMRGVVSVPP CFVRSDCFKS TAAWHTPRLR LKEKMRCERY KELRRAVQEN EHRDGVGTKV
FLPSTFTGGP HYMAEKYYDA MAVVQKLGRP DLFITMTCNS NWPEILDAVM PGQVPSDRQE
LLPRVFRGHL KQLIHEIKDK RVFRAVSAVF YTVEFQKRGL PHAHILVILD RGQKLSNPDA
MDEAISSEIP SDGELREKVS KFMIHQLCGA AKPSAACTTN KRCTKHFPKP FVRKTTFQTG
KGYPLYRRSS PDAGGCTLCE GGVVYDNGRV VPYNGYLLLR FNSHINVESC ANILACKYLY
KYILKGNDKV MVAELERQHS VNEIRMSHEV DGVDELNKEL QRCGCNRLPL YADITETHMW
HKTEKRWKLR LSAGRSGAFP KIGRLQRAYP AQGDLFYLRI LLGHEHSRGA TSFXALRTVE
HGELATFREV CQRLHLLDDD FEYDKDMETA ALHYMPHQIR QVFAMLLAYG PVSDPMALFF
KHWQPMCEDY VRKLRSMSVA PGVLQALALL DIHVQLLDMK TTMGGVGLAF PDPEAQRVAE
ELRARILLEG LPELIQEELM YDRADMERQW GANYPLLRPS RKELVDGVMS AVLNRSPLCV
FVDAPPGTGK TFCINTILAG VRRHENCIAL AVASSGIASL LLPGGRTFHS RFQAPLRPDA
KKAFPIRRQG ELVRLIRMCS LIVWDEASIT HRAQLEALNI TLQDICQTDE PFSGKVLLLA
GDFRQVLPVV RRGSRPKQIR ASIKSSPLWA HFKTHRLHEN MRVLRRGDDE VERKFAAFLL
RVGDGEHDVV DPADPETIEL PPEICMALDL GALIDWTFPD LGRHLHDPDY LTGRFVLCPK
NDAAYVINNL ILDGLPQRDI LYRSLHSAPT DSYGLNVPVE YLNSLSCSGL PPHVLRIKEG
VPIMLLRNIS VQMGMCNGTC LIVNKSVSGR LIEATILPSH KIAVIPRMTL DSDDSDGFLW
KRRQFPLHLA FAITINKSQG QSVSRAGLYL EKPVFGHGQL YVGLSRAGHP DDICVALPAD
STRTKNGRAP PASNAAHGSA LETGSTPACG SRNQSLLASA FSPDDEDFLD YDYDEVPATL
DAPSEPERCG RTPTESVRAR SPLAIHTPMG SLLLDIYQAG VDIGDLSGDE STDSWEWDRS
SPAELRGGSD GAPARYVGQS SPYGVSAGRS PLPPTTPVAA AQMNADCARA HGVSKQACEI
DAGTDNLQTP FTASTKRVRT SQPDGTSTFV PYIRPSQSSA PAVHDGAPPL RPFGSTPACG
NCGPVSVFAK LPREVVVGPS LTSDAAHRPD ASSSLARRGA TDSGQSENVV MGPMITPSYG
RNGFRPGTTL VYHDINSYDP PIWVDAKRTV ILDGKKQRIV ESWTDVLDRV GRRRIVNWML
FGLFLDGDRV EPTEHIWLDF LLARGRIERG FVPHRDKFLC DNGSLDDAHE LIEAILRHEH
ATRPGFDVPS LCEKVAKFED DYVPFEIYRY ARLDEIASRL PDVPRVSRPY AKACHGDCLI
ATYAPPVQWV SQAYIRTRTR RIAEASAARD YAHGRSPLTF SKPAANSPMT PAFTAPAARR
VQRSLFLPAT PPPFPLPAAD REAGSSACKH CSAQLSAGPS ALRQIGAVMA AKNEAVEDCS
AVEAVMFVDY CRQGYNKVAS FEQYKTAAAS YIAKDDPSIS LLSFGLHHHV CFFCHLLLYP
HAKMSPKNRG AAKDPHPLLA KSIEEEESVR WRIAELNEKL VKLRSRGYDD TDVRWLRVEE
KIDEAEEKRQ RAASRAERMR NIIDAGDIYS DADYRSGDEW IEEQEEASDS TDPEEMVTDY
DVSDLEAAVD TDYADADDDT DDDNSSSDDS TEEGDGSTNL STLCFSVLFS ALSYSRLSYP
LNGITSHDDL GTFRFRVGLS GSAGHMAIRG PLITSSCPSP QLPPTAFTLP HVLHVETASQ
IKERLEREYL TPRLDHKEGD SVVQCGLTWD VDWLSDWTRA EPTPCRDVVE PVWRPHLKTP
KLVNEADCNH HEDDRPCRCD RPDSFEQQPT PEIPEFREVE IATFNETASN SSSLLRRMGR
PEDFVRGSKS SQPFQPGGFD GGGGDEGRPR APPDAENGGW LHEVLHQTGP WQTVPPGFKH
GIRFDDEPQP EDSKKEEADK AIRMGDQHQQ VSTLSFGSIF QKAWETSYGG EEGEEEEEEE
DEEEEGENVK MRRDASGAEA SVARTAFSGR AQGEAVVELP ARPAETGVVG VGGGGGGAGV
GGGGGGGEEE AVPRIKDQDE LLDKLLSRTP GQLDMLSQKG ISDKERKEEV WAIMDGMHGI
DKRFKELVPE MAMEFPFELD TFQKEAIYRL ENYESVFVAA HTSAGKTVVA EYAFALVTKH
CTRAIYTSPI KTISNQKFRD FSGKFDVGLL TGDISIRPEA PCLIMTTEIL RSMLYRGADL
IRDVEWVVFD EVHYVNDAER GVVWEEVIIM LPDHINMIML SATVPNTKEF ADWIGRTKQK
KIFVTGTTRR PVPLEHNLFY SGKLYRICDR ETFLPEGHRE AMVAHKAKNE KKAPAGGMGQ
GRGGAGAGGR SRTGASGQTK AAAAQAARAI GSAMTGGGGG GWRSETSQWY NFLDMLKKQS
LLPVVVFCFS KNRCDQIADS LTASDLTTSQ EKSEIRVFCD RAFSRLKGTD RQLPQVLRIM
ELLKRGIGVH HAGLLPIVKE VVEMLFCRGV IKILLSTETF AMGVNAPART VAFHGLRKHD
GKTFRQILSG EYTQMAGRAG RRGLDKVGTV IILCWDDIME EGDLRRLLTG KATKLESQFR
LTYNMILNLL RVEDLKVEDM LKRSFAEFHG QKTMPLHMQE LIKREGVLNG MNTEINCILG
SPTIEEYYEM AVEADRVGEQ LMETGLQSRV AQQALVPGRV VLVRTAAESP SLGVIVRGSS
GEGQSRPYIV LALHRGPVPA SAQAMLAPAG QDVVGGGGSG GNNFPTTLPG DSPLDGMAMM
RVHRKGQKDV DYSVFSGGSK KGPEVVTIAL PRYGEVGGSG YVVMNVDKRG ILSICKARVK
VDTSAILEQP RPVAYASAVQ QLEQLERENP GQDPAALDPV KDLKLNDVET IERYRRRQAL
QAAMARNQCH RCPKLQEHYA EVRKRALLRE HVRDLKFIVS DANLQQMPEF RQRLAGGGDD
GDAAPWKDLP TMARLHRVMV QLVGGGDERA AGCRRRRQSG CTDWRLFETA CRIGAVQGSC
GLQVSPAEYA HGALKFGLME VVYEWAKGTP FADICDLTNV PEGTVVRAIV RLDELCREFR
DAARVIGDAT LFQKLEVASA SIKRDIVFAG SLYITAGQFP MLIRSGDMLA VAIRVQI
//
