ID A0A388KVI1_CHABU Unreviewed; 2335 AA.
AC A0A388KVI1;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=Reverse transcriptase domain-containing protein {ECO:0008006|Google:ProtNLM};
GN ORFNames=CBR_g17791 {ECO:0000313|EMBL:GBG74080.1};
OS Chara braunii (Braun's stonewort).
OC Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae;
OC Chara.
OX NCBI_TaxID=69332 {ECO:0000313|EMBL:GBG74080.1, ECO:0000313|Proteomes:UP000265515};
RN [1] {ECO:0000313|EMBL:GBG74080.1, ECO:0000313|Proteomes:UP000265515}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S276 {ECO:0000313|EMBL:GBG74080.1,
RC ECO:0000313|Proteomes:UP000265515};
RX PubMed=30007417; DOI=10.1016/j.cell.2018.06.033;
RA Nishiyama T., Sakayama H., Vries J.D., Buschmann H., Saint-Marcoux D.,
RA Ullrich K.K., Haas F.B., Vanderstraeten L., Becker D., Lang D.,
RA Vosolsobe S., Rombauts S., Wilhelmsson P.K.I., Janitza P., Kern R.,
RA Heyl A., Rumpler F., Villalobos L.I.A.C., Clay J.M., Skokan R., Toyoda A.,
RA Suzuki Y., Kagoshima H., Schijlen E., Tajeshwar N., Catarino B.,
RA Hetherington A.J., Saltykova A., Bonnot C., Breuninger H., Symeonidi A.,
RA Radhakrishnan G.V., Van Nieuwerburgh F., Deforce D., Chang C., Karol K.G.,
RA Hedrich R., Ulvskov P., Glockner G., Delwiche C.F., Petrasek J.,
RA Van de Peer Y., Friml J., Beilby M., Dolan L., Kohara Y., Sugano S.,
RA Fujiyama A., Delaux P.-M., Quint M., TheiBen G., Hagemann M., Harholt J.,
RA Dunand C., Zachgo S., Langdale J., Maumus F., Straeten D.V.D., Gould S.B.,
RA Rensing S.A.;
RT "The Chara Genome: Secondary Complexity and Implications for Plant
RT Terrestrialization.";
RL Cell 174:448-464(2018).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004606}; Single-
CC pass type II membrane protein {ECO:0000256|ARBA:ARBA00004606}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GBG74080.1}.
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DR EMBL; BFEA01000196; GBG74080.1; -; Genomic_DNA.
DR EnsemblPlants; GBG74080; GBG74080; CBR_g17791.
DR Gramene; GBG74080; GBG74080; CBR_g17791.
DR Proteomes; UP000265515; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR CDD; cd09274; RNase_HI_RT_Ty3; 2.
DR CDD; cd01647; RT_LTR; 1.
DR Gene3D; 3.30.70.270; -; 4.
DR Gene3D; 3.10.10.10; HIV Type 1 Reverse Transcriptase, subunit A, domain 1; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR016197; Chromo-like_dom_sf.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR003406; Glyco_trans_14.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR000477; RT_dom.
DR InterPro; IPR041373; RT_RNaseH.
DR InterPro; IPR041577; RT_RNaseH_2.
DR PANTHER; PTHR37984:SF7; INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR37984; PROTEIN CBG26694; 1.
DR Pfam; PF02485; Branch; 1.
DR Pfam; PF17917; RT_RNaseH; 1.
DR Pfam; PF17919; RT_RNaseH_2; 1.
DR Pfam; PF00078; RVT_1; 1.
DR SUPFAM; SSF54160; Chromo domain-like; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 2.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000265515};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 1770..1796
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1817..1837
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 286..389
FT /note="Reverse transcriptase RNase H-like"
FT /evidence="ECO:0000259|Pfam:PF17917"
FT DOMAIN 1110..1250
FT /note="Reverse transcriptase"
FT /evidence="ECO:0000259|Pfam:PF00078"
FT DOMAIN 1308..1402
FT /note="Reverse transcriptase/retrotransposon-derived
FT protein RNase H-like"
FT /evidence="ECO:0000259|Pfam:PF17919"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 65..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 726..760
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1685..1704
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 124..176
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 7..21
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 735..760
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2335 AA; 264382 MW; 604C831537D8F612 CRC64;
MVDLHSGKST TPYTKAQEEQ AATVLQERKE KEAKKELIKQ AKKLALQVEQ AAKRKKLGEE
MERLKKEEEE KMKAVEEEEI EEEKEEEPLI RRSAGQRGES KKVFEWVANL SLGEEEEAML
YVPREEKEAV IRELEEEEDP LMRQTIEDEK KLQRKLRLTT EKKRRMDEVL EKLREANFKI
NAKKCEWAKT QVLYLGHVLD GDDIKPKDRK IAVIRDWPTP RTLTELRSFL GLANYYRKLA
NFSTIAAPLR RLLKKEAIWK WDKDCTSALK KLKRALIEYP VLKVADPSLP FVVTTDASQY
GIGAVLHQDD GNGYRSVEFM PARMPSKKVA TSTYERELYA LRQALQHWKH YLLGRHFKVY
SDHETLRWLK TQAKMTSKLT RWAATLYQYD FELKPVKGKY NVVADALRRR ADYFGAIVHY
LDIGRDLQQK IQRLPPGTRE FSIQHEKEIG TAVDNLRKAQ HRMIEQANKH RRPSQFQVGD
LVWVKSKEFA PEENISQKLL PAYRGPWPVL EVKGGEDGPS YTIEIPAHLH MYPMYHASKL
LPCSTSPQIP SRRSMIPPDM DGRYDIDGTV GSLNSRDITC ASFVRMRQIG AQINTMVPTG
ETCFLEPLQV AEWKPGNARL TSPCYGSKLE LMSMEQDTGA LPRRSARLAV RARSVVPPRT
KYQQQRISRR TTPAASNTTL TVLVPIGVLP ACPVQGAHEP LADYLGRLQV FTDAVATAKA
QQEAAEAERQ RLANEAAAQA QQTAEADAAA QDRRNAASTE SLIQSENQWT TLLQGMFFVP
TGARAAATQQ QQQQQLLNST LARINNIEAK TSAAPGCTTD ATKQLNERID HVVTIIGELG
DFTSPATISS TAAAIKTSIT KLQTRLDAAT KNYKMPHFDV SKFDDYNKSD ALTWWQSFLT
EASCRTVPAD DMMKALYLQL IGGVQAWMNH LAATKKCTIA ELHTHITWKE FEKLWFTRFM
VRNVVKAAMN EVYTCSQGSM PTRDWTTKWQ KIVTTPGFDL TRNDVEEMGL VFLHALPSPD
GPAASPSDPR ITHLLNEYGD VFEAPTGTVP DRPIRHGITL EVGAVPPRGC IYRMSEEELE
VLRAQLDDLL DKGWIRPSCS PYGAPILFVR KKNKDLRLCI NYRNAQTVKN VGPLPRIDDL
LERLGGATYF SKLDLKSGYH QIEIQPQDRY KTAFKTRYGH FEWVVMPFGL TNAPATFQAA
MTTEFRDLLD RSVLIYLDDI LVYSRTLDEH IVHLRTVLDH LRLAKYKANS TSIRPLADKI
QAIVDWPEPR CTTDVRSFMG LAGYYQRFVE SYSKVAAPLS RLQSFEFDDA ARGAFTTLKA
TMQVAPALRM YDPTLPTQVT TDASGYDIGA VLEQCHEDGW HPVEYFSQKV PLVNTLDDAR
KRELLAFVTV LKQWRHFLFG RRRVKWNTDN NPLTFYKTQD TVTSTIGRWM HYIDQFDFDP
CHIPAPELAR VLHTGWITSH GVQEDIVSDR DTRFMSAFST SLMAESGTTM KPSSARHLQM
DGQTERAHQT AQMMLRIRPD QKDWADRLLD IEFAYNTSVH PAICVTPFEL HHGGEKARIF
ADLLLPQAAN IDVPCSPASV RKYRDLLIKV RANMQKAQIC MQQQANRRRL PCPFCEGDLV
WVLSEEFALE QDVSRKLLPK WFGPWEVTSA VGDDPAGPSF VVNIPPHLTV HRVFHPSKLA
IYTPPSADEF PGRRSQDPPS MDGHQEVDRV ITHRKYGNKP MQFKVIFRQC APDDTRWISS
ADLQTSSPLI FADYEKRRLA KEAARPTRPI LVFGGLLSSC IALGLVASCS FTEVVQRRLL
FLTMAITVPR RVSMRKSVLA GGVVAAVLLV IFVFLAFNSL SLSLQAKTVL QNGLSGTPGS
GILEGDFISV HGSVVARRAA DLANNRFVAC TNWCKEANDR AHQCILQSGT SEDINAVNNS
SPAAEDLLTA SVEGYEELSA RDVLQVTGSP NQQQQQQQMT DTGLQLGRAA SAGVDPFCYG
TANHSAWGKM DDRLTGSGSV QRFLYMMSLA SQAVFGGRAK ESSADPSQFA NMNTADIELL
EVASERANLA PKIAFMFLVR DSLTFAPLWE KIFHGHENQY SVYVHATEAY KRGPQESDVF
EKSVICSKPV KWGRPSLVDA ERRLIASAIL DPLNQKFVLL SESCIPVRNF SFIYSYLMDT
SKSFVEIGLF PDRYPPELAP LVKVEQFRKG SQWVTLNRVH AALVVADRVY YPPFMRWCQG
FPRRFCIGFA TKRRCWNFPN VLCFADEHYM QTMLHILDEG AVHPRTLTWL EWIWQSPHPR
TRGAGDTDLA LLKQIQNDAT CEWNGLNREC FLFARKFSKD ALPIILSLGK QLQLW
//
