ID A0A388LF35_CHABU Unreviewed; 2164 AA.
AC A0A388LF35;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=RNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012493};
DE EC=2.7.7.49 {ECO:0000256|ARBA:ARBA00012493};
GN ORFNames=CBR_g31485 {ECO:0000313|EMBL:GBG80929.1};
OS Chara braunii (Braun's stonewort).
OC Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae;
OC Chara.
OX NCBI_TaxID=69332 {ECO:0000313|EMBL:GBG80929.1, ECO:0000313|Proteomes:UP000265515};
RN [1] {ECO:0000313|EMBL:GBG80929.1, ECO:0000313|Proteomes:UP000265515}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S276 {ECO:0000313|EMBL:GBG80929.1,
RC ECO:0000313|Proteomes:UP000265515};
RX PubMed=30007417; DOI=10.1016/j.cell.2018.06.033;
RA Nishiyama T., Sakayama H., Vries J.D., Buschmann H., Saint-Marcoux D.,
RA Ullrich K.K., Haas F.B., Vanderstraeten L., Becker D., Lang D.,
RA Vosolsobe S., Rombauts S., Wilhelmsson P.K.I., Janitza P., Kern R.,
RA Heyl A., Rumpler F., Villalobos L.I.A.C., Clay J.M., Skokan R., Toyoda A.,
RA Suzuki Y., Kagoshima H., Schijlen E., Tajeshwar N., Catarino B.,
RA Hetherington A.J., Saltykova A., Bonnot C., Breuninger H., Symeonidi A.,
RA Radhakrishnan G.V., Van Nieuwerburgh F., Deforce D., Chang C., Karol K.G.,
RA Hedrich R., Ulvskov P., Glockner G., Delwiche C.F., Petrasek J.,
RA Van de Peer Y., Friml J., Beilby M., Dolan L., Kohara Y., Sugano S.,
RA Fujiyama A., Delaux P.-M., Quint M., TheiBen G., Hagemann M., Harholt J.,
RA Dunand C., Zachgo S., Langdale J., Maumus F., Straeten D.V.D., Gould S.B.,
RA Rensing S.A.;
RT "The Chara Genome: Secondary Complexity and Implications for Plant
RT Terrestrialization.";
RL Cell 174:448-464(2018).
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GBG80929.1}.
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DR EMBL; BFEA01000360; GBG80929.1; -; Genomic_DNA.
DR EnsemblPlants; GBG80929; GBG80929; CBR_g31485.
DR Gramene; GBG80929; GBG80929; CBR_g31485.
DR Proteomes; UP000265515; Unassembled WGS sequence.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR CDD; cd09274; RNase_HI_RT_Ty3; 2.
DR CDD; cd01647; RT_LTR; 1.
DR Gene3D; 1.10.340.70; -; 1.
DR Gene3D; 3.10.20.370; -; 1.
DR Gene3D; 3.30.70.270; -; 2.
DR Gene3D; 2.40.70.10; Acid Proteases; 1.
DR Gene3D; 3.10.10.10; HIV Type 1 Reverse Transcriptase, subunit A, domain 1; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR001584; Integrase_cat-core.
DR InterPro; IPR041588; Integrase_H2C2.
DR InterPro; IPR021109; Peptidase_aspartic_dom_sf.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR000477; RT_dom.
DR InterPro; IPR041373; RT_RNaseH.
DR InterPro; IPR041577; RT_RNaseH_2.
DR PANTHER; PTHR37984:SF7; INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR37984; PROTEIN CBG26694; 1.
DR Pfam; PF17921; Integrase_H2C2; 1.
DR Pfam; PF17917; RT_RNaseH; 1.
DR Pfam; PF17919; RT_RNaseH_2; 1.
DR Pfam; PF00665; rve; 1.
DR Pfam; PF00078; RVT_1; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 3.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS50994; INTEGRASE; 1.
DR PROSITE; PS50878; RT_POL; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Reference proteome {ECO:0000313|Proteomes:UP000265515}.
FT DOMAIN 1585..1766
FT /note="Reverse transcriptase"
FT /evidence="ECO:0000259|PROSITE:PS50878"
FT DOMAIN 2040..2164
FT /note="Integrase catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50994"
FT REGION 524..548
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1014..1060
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1238..1259
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 908..952
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 1016..1042
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2164 AA; 245956 MW; AEB46007DFBB78EE CRC64;
MSLTRTSTSF WGCLGLQVRI TRLTSISGHL PCDAFGADVS CTIPLPHPLI RCQVVTAKSF
RATCAHERTD EIGLCFLRAV AMNESSPTNL SSDPLVVRLL DEFTDIFESP TGVVPDRSIS
YEIILEAGVV PPKGCIYRMS EEELAVLRTQ LDDLLDKSWI RPSISPLSEK IQAIQEWSDP
RNVTDVCSFL GLAGYYQRFI KGYSKIAAHL TKLQCEDRSF DFREDARESF LALKVVLLSA
ELLRIYDPLL PTRVTTDASG YGIGAVLEQH DGVDWHPAEY FTKKEPVVHS IDDARKKELL
AFVHSLKRWR HFLLGRSQFL WVTDNNPLVF YKTQDTVNGT IARWMAFIDQ FDFFLDHIPG
KFNRFADALS RRPDHCTAVY STFEIDDDLR DSFIRGYQAD TEFRDKYAFC SSPNPMPSHY
QIQEGYLPVH TWGKDLLCVP TPGPFTPCDE DDVCWVCIVT LVGLVIGILA FRYAVLYYVR
ECMPRLTRSQ TKTMDRKEGE SLLAYEEHLA AFIQEANNRA AAAAKERGRI EQEEEAKRQK
EEQDRLRQEE ADLQAIAEHW SRQRERLFTR ETVIGDEAAH WVQVASTDEA PETEKGLSAL
AQVSHDLVAT CVLQQEEILH LQQTVDQMLA RLQALEKQPA VVAAAGPSNL ATRVQVLKDD
VSNIKRVHQD FRTSQQATNV QLETQVQAAA TVTPAAASSR CPPKLDGIPV FCDQSKTEPI
SWWRQFTLRL DMHHVPNNDR HPCLYHQSGG ACQAWLDNIL TSHGVAVSKL HTKLTWQELT
DAWHKRFQVE PPDLQAMDKL NKLHQNTLLS QDWITEFQRL ASIPDPPLTF RAIKHSFIMR
SCPALQNVLT QIAETLDTSD KLFSTASRII LTNIEARNAG RSFMTTSGTQ PKPKFSPLTP
RGRELLELQQ VERIRLKLER ELKQATDREN KIKQRAARLE TLEADKAELD ESVLSDQMKV
LKNNMLSLHA HVDNRLDFMQ NTLDQILDIL QRPGFRPPAQ SPLPLTAMFG PFPVQAGTQP
SGTSAAAAQT VASSSSGPAV GATPPQQPVP QQGQPQGQWY PKTPMKPPLA FSGERKDEEL
NTWLRTVPVW VKAKRTLPED EVDTAASYLE GKTAKWLDGI VAKAGYGRRM ADWAQSITLD
QFFEMVEARW HNPQQAQIAT DAINRLDQRK FKSVRELTTT IESLIVIPGI NYSDQFMLTT
FVRCLPENLR NLLASEARLE YHSFKTLFRK ALDLETTLGN TQPSQNDARK KKSPQEWKKK
GAKLMLVESD GTQTEIDKLT NLLDGSEYDG EKTAEGSTLA AVVKAKAGGR VKGPPKSQGK
AASNQTKVAD WVTTGLDQDV WRDRRVRGAC ISCALEDLES EWSNKDPRES WVALRKGPRG
EHFVVEVDVG GRKCGAFIDI GSTRNYISRD CLEIKDVVCT FSSGGGKLNH KISFLVSDDL
PFDMLLGMYY LEVAKPQFDW DKKVLKHKLP DGRTVRLTKF KASSIIDTYG CLCASAFYNY
YKQNQEEGMY LVYVSKKGEA IKTPPEIERV VAKFPDLFEE PTGVVEREVV HAIEIIPGSK
TPKERIYRMA PAELDELRRQ LKEPTKKGWI WPSTSPYGSP VLFVPKKGGT LRMCIDYGGR
NAITVKNTEP LPRIDDLLDR VQGCKYYSKI DLKSGYHQIA IRPEDQHKTT FQTRYGLYEF
VVMPFGLCNA SGTFQHAMNR IFHDHLDKFV VVYLDDILIF SKSAEEHAQH VETVRSLLRQ
HKYKVNLEKY WSDECEGAFK RLKHALMNHE VFMVLDPQKP FIVTTDASQY GIGAVLAQQD
GKKLRPLEYM SKKMPSKKLA KSTYERELYA LYKALVHWRH FLLGRFFYLR TDHHTLKWIK
THPALSDALK RWIEVIDQYD FKLKYLKGEY NKVADALLRR VDYLGAQVSD FGVSEEVTQS
LVGAYQEDPV TMDIMRKLQA KDKATESEFV MVDGLLYLDK AGVKRLIVPS NERLRSLFLG
ECHDATRHFG YKKTSVNLVQ RFWWPGMLDD AKKYVETCQV CQRDKPRTQA PLGLMKPLPI
PAGPRQSVSM DFMDTLVTSK SGKRHIFIII DRFTKYARLV AMPETARTDH VIKLFQDNWV
GDFGLPKTIV SDRDVRFTSE LWKKTAEQMG SQLQMTSRNH PEANGQAEQM NRVVQHLLRH
YSTC
//
