ID A0A388LME3_CHABU Unreviewed; 529 AA.
AC A0A388LME3;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=UPF0020 domain-containing protein {ECO:0000259|Pfam:PF01170};
GN ORFNames=CBR_g37214 {ECO:0000313|EMBL:GBG83500.1};
OS Chara braunii (Braun's stonewort).
OC Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae;
OC Chara.
OX NCBI_TaxID=69332 {ECO:0000313|EMBL:GBG83500.1, ECO:0000313|Proteomes:UP000265515};
RN [1] {ECO:0000313|EMBL:GBG83500.1, ECO:0000313|Proteomes:UP000265515}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S276 {ECO:0000313|EMBL:GBG83500.1,
RC ECO:0000313|Proteomes:UP000265515};
RX PubMed=30007417; DOI=10.1016/j.cell.2018.06.033;
RA Nishiyama T., Sakayama H., Vries J.D., Buschmann H., Saint-Marcoux D.,
RA Ullrich K.K., Haas F.B., Vanderstraeten L., Becker D., Lang D.,
RA Vosolsobe S., Rombauts S., Wilhelmsson P.K.I., Janitza P., Kern R.,
RA Heyl A., Rumpler F., Villalobos L.I.A.C., Clay J.M., Skokan R., Toyoda A.,
RA Suzuki Y., Kagoshima H., Schijlen E., Tajeshwar N., Catarino B.,
RA Hetherington A.J., Saltykova A., Bonnot C., Breuninger H., Symeonidi A.,
RA Radhakrishnan G.V., Van Nieuwerburgh F., Deforce D., Chang C., Karol K.G.,
RA Hedrich R., Ulvskov P., Glockner G., Delwiche C.F., Petrasek J.,
RA Van de Peer Y., Friml J., Beilby M., Dolan L., Kohara Y., Sugano S.,
RA Fujiyama A., Delaux P.-M., Quint M., TheiBen G., Hagemann M., Harholt J.,
RA Dunand C., Zachgo S., Langdale J., Maumus F., Straeten D.V.D., Gould S.B.,
RA Rensing S.A.;
RT "The Chara Genome: Secondary Complexity and Implications for Plant
RT Terrestrialization.";
RL Cell 174:448-464(2018).
CC -!- FUNCTION: Catalytic subunit of an S-adenosyl-L-methionine-dependent
CC tRNA methyltransferase complex that mediates the methylation of the
CC guanosine nucleotide at position 10 (m2G10) in tRNAs.
CC {ECO:0000256|ARBA:ARBA00002425}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GBG83500.1}.
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DR EMBL; BFEA01000441; GBG83500.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A388LME3; -.
DR STRING; 69332.A0A388LME3; -.
DR EnsemblPlants; GBG83500; GBG83500; CBR_g37214.
DR Gramene; GBG83500; GBG83500; CBR_g37214.
DR OMA; QAFNKWS; -.
DR Proteomes; UP000265515; Unassembled WGS sequence.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0080180; P:2-methylguanosine metabolic process; IEA:EnsemblPlants.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006400; P:tRNA modification; IEA:EnsemblPlants.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR000241; RlmKL-like_Mtase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR016691; tRNA_mtfrase_TRM11.
DR PANTHER; PTHR13370; RNA METHYLASE-RELATED; 1.
DR PANTHER; PTHR13370:SF3; TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG; 1.
DR Pfam; PF01170; UPF0020; 1.
DR PIRSF; PIRSF017259; tRNA_mtfrase_TRM11; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
DR PROSITE; PS51627; SAM_MT_TRM11; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000265515};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022555};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555}.
FT DOMAIN 218..339
FT /note="UPF0020"
FT /evidence="ECO:0000259|Pfam:PF01170"
FT REGION 32..55
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 490..529
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 36..55
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 529 AA; 59478 MW; F4D50ACE80288B44 CRC64;
MWYLCYFIHR HLDFRIAEVE SLARIAGCCS SVDDSNPRSP PPPPPPSPPS PPPSPRLAWR
LPDGDHPDSP FWFVDLPSND AALAIVRRGI LLKGVFEVWG EGATYDELEN SVKSYLTVEK
EKTAAYLTAG SSFKIVVDGF GKIFSMQEQI QRIERLSYIP FQGKVNLKSP DHKFWLIESV
VGEGVNNGLP PRVPRRIYFA REVGCSNRTI VSTYTLSKRR YLGPTSMDAE MAFIMANQAL
AMAGKLIYDP FVGTGSVLIA AAHYGAYTVG ADIDIRVVRD GRGRDRNIWS NFQQYGLMPP
LSLLRVDNNV NPWRTDLDEV FDSVLCDPPY GVRAGGRKSG GRKMLNGRVE PYVIPEEMKS
DHIPSTAPYT LEECLQDMLD MASRLLVMGG RLVFFFPVLR EEYKEEEDLP RHACLHLVAN
SEQILTRRWS RRLLTYEKVT RYTADMAKEA RAKHEYFRIN HLKFLEEARA KADLRAAVFA
PRRAEWFPDL ESGSPSMDGG GGGDGTVNGA NEHSVATAAR RPRYRGKNV
//
