ID A0A395N1W6_9HYPO Unreviewed; 1426 AA.
AC A0A395N1W6;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=alpha-1,2-Mannosidase {ECO:0000256|RuleBase:RU361193};
DE EC=3.2.1.- {ECO:0000256|RuleBase:RU361193};
GN ORFNames=FIE12Z_1597 {ECO:0000313|EMBL:RFN54108.1};
OS Fusarium flagelliforme.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC Fusarium incarnatum-equiseti species complex.
OX NCBI_TaxID=2675880 {ECO:0000313|EMBL:RFN54108.1, ECO:0000313|Proteomes:UP000265631};
RN [1] {ECO:0000313|EMBL:RFN54108.1, ECO:0000313|Proteomes:UP000265631}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL 13405 {ECO:0000313|EMBL:RFN54108.1,
RC ECO:0000313|Proteomes:UP000265631};
RX PubMed=29649280; DOI=10.1371/journal.ppat.1006946;
RA Proctor R.H., McCormick S.P., Kim H.S., Cardoza R.E., Stanley A.M.,
RA Lindo L., Kelly A., Brown D.W., Lee T., Vaughan M.M., Alexander N.J.,
RA Busman M., Gutierrez S.;
RT "Evolution of structural diversity of trichothecenes, a family of toxins
RT produced by plant pathogenic and entomopathogenic fungi.";
RL PLoS Pathog. 14:e1006946-e1006946(2018).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 47 family.
CC {ECO:0000256|ARBA:ARBA00007658, ECO:0000256|RuleBase:RU361193}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RFN54108.1}.
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DR EMBL; PXXK01000030; RFN54108.1; -; Genomic_DNA.
DR STRING; 2594813.A0A395N1W6; -.
DR UniPathway; UPA00378; -.
DR Proteomes; UP000265631; Unassembled WGS sequence.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0012505; C:endomembrane system; IEA:UniProt.
DR GO; GO:0030117; C:membrane coat; IEA:InterPro.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004571; F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0016482; P:cytosolic transport; IEA:UniProt.
DR GO; GO:0016197; P:endosomal transport; IEA:UniProt.
DR GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR GO; GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway.
DR GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; IEA:UniProt.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 2.60.40.1230; -; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR002553; Clathrin/coatomer_adapt-like_N.
DR InterPro; IPR008152; Clathrin_a/b/g-adaptin_app_Ig.
DR InterPro; IPR013041; Clathrin_app_Ig-like_sf.
DR InterPro; IPR008153; GAE_dom.
DR InterPro; IPR001382; Glyco_hydro_47.
DR InterPro; IPR036026; Seven-hairpin_glycosidases.
DR PANTHER; PTHR22780; ADAPTIN, ALPHA/GAMMA/EPSILON; 1.
DR PANTHER; PTHR22780:SF5; AP-1 COMPLEX SUBUNIT GAMMA; 1.
DR Pfam; PF01602; Adaptin_N; 1.
DR Pfam; PF02883; Alpha_adaptinC2; 1.
DR Pfam; PF01532; Glyco_hydro_47; 1.
DR PRINTS; PR00747; GLYHDRLASE47.
DR SMART; SM00809; Alpha_adaptinC2; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF49348; Clathrin adaptor appendage domain; 1.
DR SUPFAM; SSF48225; Seven-hairpin glycosidases; 1.
DR PROSITE; PS50180; GAE; 1.
PE 3: Inferred from homology;
KW Cytoplasmic vesicle {ECO:0000256|ARBA:ARBA00023329};
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR601382-3};
KW Glycosidase {ECO:0000256|RuleBase:RU361193};
KW Hydrolase {ECO:0000256|RuleBase:RU361193};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Protein transport {ECO:0000256|ARBA:ARBA00022927};
KW Reference proteome {ECO:0000313|Proteomes:UP000265631};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT TRANSMEM 9..26
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 1315..1426
FT /note="GAE"
FT /evidence="ECO:0000259|PROSITE:PS50180"
FT REGION 1201..1320
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1240..1254
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1270..1310
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 179
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT ACT_SITE 317
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT ACT_SITE 436
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT ACT_SITE 514
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT DISULFID 393..422
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-3"
SQ SEQUENCE 1426 AA; 158321 MW; C56D64C0BF13A591 CRC64;
MAGPLRRGRL WAGVIFIWIF CFWYWSSSDS TFSLFGNSDP LAGKGASAWT RRLPKYPIPQ
EKLAVLPEIK GDVKVPKIQA TAPVESAAAK ELRLSRLAAV KKSFEHSWSG YSNYAWMHDE
VTPLTGKSKD PFGGWAATLV DALDTLWIMD MKEEFTKAVA AADGIDFTRS HMTTINIFET
TIRYLGGFLS AYELSGKSHP ILLKKAIELG DLLMVAFDTP NHIPITRLEW KKAAYGQSQS
ASRETLVSEL GSMSLELTKL SQITGNMKYY DAVKRLGDQF ESTQLNTRLP GMWPVVVDAY
TPAFHAGSEF TLGGMSDSLY EYLPKWYLLL GGQLEQPRRL YENFIPVAIK HLFKRALTPS
EKPIIISGDY QVTDLPGQQP KYTSVARGQH LTCFAGGMMA MASKIFNRPA DLDIATQLTD
GCIWAYKATQ TGLGPEVFSF ISCGSVDVKE TGDCTWSEER WLKAIEEQHA PDFKAPPMKG
PEWKQPTVQD VVKKHNLPKG MIDVSDPRYI LRPEAIESIF IMYRTTGDAQ WMEKAWSMFE
TIEKVTRTEI AASAIDDVTK AEPTKMDSME SFWLAETLKY FYLIFSEFDV ISLDQDLLFL
FLRIVKQFIR NVRAAKTIAD ERAVIQKESA AIRASFREES HDPNVRRNNV AKLLYLFTLG
ERTHFGQIEC LKLLASPRFA DKRLGHLATS LLLDENQEVL TLVTNSLKND LGHSNQYVVG
LALCTLGNIA SIEMSRDLFP EIETLVATAN PYIRRKAALC AMRICRKVPD LQEHFIEKAT
QLLSDRNHGV LLCGLTLVTS LCEADEEEGG EEGIVEKFRS FVPGLVRTLK GLATSGYAPE
HDVTGITDPF LQVKILHLLR VLAVGDAETS EQINDILAQV ATNTESSKNV GNSILYEAVR
TILDIEADSG LRVLGVNILG KFLTNRDNNI RYVALNTLIK VVAIEPNAVQ RHRNTILECL
RDPDISIRRR ALDLSFTLIN ESNVRVLIRE LLAFLEVADN EFKPTMTSQI GIAADKFSPN
KRWHFDTMLR VLSLAGNYVK EQILSSFVRL VATTPELQTY AVQKLYINLK KDITQESLTQ
AGAWCIGEYA DALLKGGQYE EEELVQEVKE HEVVDLFSLI LNSAYATQVS TEYIVTALMK
LTTRFSDPAQ IEKIRRILQY HQTSLDIEIQ QRVVEYGNLF SFDQVRRGVL EKMPIPQIKE
ESRVLGSAPT KKKASNRKSR VIKPTEQDLL DIMDAPAATP AAPSTTNTDL LADILGGTSS
PPPSAASPPP QSNMSSIMDM FGSAPGSSTA SPAPPSSNFD LMSSGAAAPQ PQAPQAPAGI
PCYNSNDLNV TFQIQRNAEG MIQATAKFIN TSGSATLSNV SLQAAVPKSQ KLQLLSISSS
DLGPGAEASQ MMRVSGCKGP LRLRLRIGYT HPTAGQVMDQ VNWTES
//
