ID A0A395N215_9HYPO Unreviewed; 480 AA.
AC A0A395N215;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE RecName: Full=Vacuolar calcium ion transporter {ECO:0000256|RuleBase:RU365028};
GN ORFNames=FIE12Z_1651 {ECO:0000313|EMBL:RFN54162.1};
OS Fusarium flagelliforme.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC Fusarium incarnatum-equiseti species complex.
OX NCBI_TaxID=2675880 {ECO:0000313|EMBL:RFN54162.1, ECO:0000313|Proteomes:UP000265631};
RN [1] {ECO:0000313|EMBL:RFN54162.1, ECO:0000313|Proteomes:UP000265631}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL 13405 {ECO:0000313|EMBL:RFN54162.1,
RC ECO:0000313|Proteomes:UP000265631};
RX PubMed=29649280; DOI=10.1371/journal.ppat.1006946;
RA Proctor R.H., McCormick S.P., Kim H.S., Cardoza R.E., Stanley A.M.,
RA Lindo L., Kelly A., Brown D.W., Lee T., Vaughan M.M., Alexander N.J.,
RA Busman M., Gutierrez S.;
RT "Evolution of structural diversity of trichothecenes, a family of toxins
RT produced by plant pathogenic and entomopathogenic fungi.";
RL PLoS Pathog. 14:e1006946-e1006946(2018).
CC -!- FUNCTION: Has a role in promoting intracellular calcium ion
CC sequestration via the exchange of calcium ions for hydrogen ions across
CC the vacuolar membrane. Involved also in manganese ion homeostasis via
CC its uptake into the vacuole. {ECO:0000256|RuleBase:RU365028}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}. Vacuole membrane
CC {ECO:0000256|RuleBase:RU365028}.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. {ECO:0000256|ARBA:ARBA00008170, ECO:0000256|RuleBase:RU365028}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|RuleBase:RU365028}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RFN54162.1}.
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DR EMBL; PXXK01000030; RFN54162.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A395N215; -.
DR STRING; 2594813.A0A395N215; -.
DR OrthoDB; 276371at2759; -.
DR Proteomes; UP000265631; Unassembled WGS sequence.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015369; F:calcium:proton antiporter activity; IEA:UniProtKB-UniRule.
DR Gene3D; 1.20.1420.30; NCX, central ion-binding region; 2.
DR InterPro; IPR004713; CaH_exchang.
DR InterPro; IPR004798; CAX-like.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR NCBIfam; TIGR00378; cax; 1.
DR PANTHER; PTHR31503; VACUOLAR CALCIUM ION TRANSPORTER; 1.
DR PANTHER; PTHR31503:SF14; VACUOLAR CALCIUM ION TRANSPORTER; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
PE 3: Inferred from homology;
KW Antiport {ECO:0000256|RuleBase:RU365028};
KW Calcium {ECO:0000256|RuleBase:RU365028};
KW Calcium transport {ECO:0000256|RuleBase:RU365028};
KW Ion transport {ECO:0000256|RuleBase:RU365028};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU365028};
KW Reference proteome {ECO:0000313|Proteomes:UP000265631};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU365028};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU365028}; Transport {ECO:0000256|RuleBase:RU365028};
KW Vacuole {ECO:0000256|RuleBase:RU365028}.
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 119..138
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 150..172
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 178..203
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 215..237
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 309..333
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 411..432
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 439..459
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT DOMAIN 119..278
FT /note="Sodium/calcium exchanger membrane region"
FT /evidence="ECO:0000259|Pfam:PF01699"
FT DOMAIN 315..457
FT /note="Sodium/calcium exchanger membrane region"
FT /evidence="ECO:0000259|Pfam:PF01699"
FT REGION 1..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 9..23
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 27..41
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 480 AA; 52898 MW; 71DCA1E57EB7B406 CRC64;
MPRDHRRERS SSRSHHPRHR SRSHNSPDMR QVSSDGRTLP VPNTYSEKRG DRGRNGNGNG
KMSRYIKPAG ESGRSGFHPI HFSKISFRST SRASLLCNFL WPFVPAAIAV RYAMPDNHVT
IFALAYIAMI PCANLIGFAG QELSRKLPHV WGVLIEITIG SIVEIILFMV LLSKNMYYVI
KAAILGSILA TMLLCLGCCF FVGGMLEDEQ VFSEAISEAG SGLLLTAGVV LALPTVFEYG
VGNGETLTSQ SLEHKTLQIS RIVSILLIIA YLVYVFFQAR THHGIYDAVF EQDEHNDRDK
HKDQAKAKLT LTECIIALVI SVGLVAWTAV ILVMQIEYVI ERGHVSDAFM GLILVPLVEK
LAEHLTAIDE AWDNQINLAM SHVLGATLQT ALFNAPLTVI VSWGLHKTLD LNFAVFDLVM
LILAILTVGR FLQDQKSNYL EGFLLIILYV AIAVAAFYYP DPAHHGAGGE SSEGSSEGGH
//
