ID A0A396QTZ4_9FIRM Unreviewed; 497 AA.
AC A0A396QTZ4;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=DNA polymerase I {ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460,
GN ECO:0000313|EMBL:RHV92283.1};
GN ORFNames=DXA96_00610 {ECO:0000313|EMBL:RHV92283.1};
OS Lachnospiraceae bacterium OF09-33XD.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae.
OX NCBI_TaxID=2292273 {ECO:0000313|EMBL:RHV92283.1, ECO:0000313|Proteomes:UP000265865};
RN [1] {ECO:0000313|EMBL:RHV92283.1, ECO:0000313|Proteomes:UP000265865}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OF09-33XD {ECO:0000313|EMBL:RHV92283.1,
RC ECO:0000313|Proteomes:UP000265865};
RA Zou Y., Xue W., Luo G.;
RT "A genome reference for cultivated species of the human gut microbiota.";
RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SUBUNIT: Single-chain monomer with multiple functions.
CC {ECO:0000256|RuleBase:RU004460}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RHV92283.1}.
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DR EMBL; QUME01000001; RHV92283.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A396QTZ4; -.
DR Proteomes; UP000265865; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06140; DNA_polA_I_Bacillus_like_exo; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|RuleBase:RU004460};
KW Nucleotidyltransferase {ECO:0000256|RuleBase:RU004460,
KW ECO:0000313|EMBL:RHV92283.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000265865};
KW Transferase {ECO:0000256|RuleBase:RU004460, ECO:0000313|EMBL:RHV92283.1}.
FT DOMAIN 255..461
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 497 AA; 55977 MW; CBFA30BA73E57854 CRC64;
MKYARAPRQE GFFDIQIAAY LLNPLKSVYT YDDIAKEYLG AVIPSAEDIF GSVKLPGASV
MTPEQAACYA GQTAFVSLAA REPLSGALEE QHMKQLFLEI EMPLVFTLAD MEEAGIEVHA
EELQEYGRNL QVRIDEVEKK IYEQAGETFN INSPKQLGVI LFEKLKLTGG KKTKSGYSTA
ADVLERLAPD APVVADILEY RQLTKLKSTY ADGLMNYVAA DGRIHSSFNQ TITATGRISS
TDPNLQNIPV RMELGRQIRK VFVAKEGCVF IDADYSQIEL RVLAHLSGDE KLIQAYREAS
DIHRITASEV FHVPFDQVTP LQRRNAKAVN FGIVYGISSF GLSQDLSISR KEAADYIEKY
FQTYPKIKEF LDRTVKEAKD KGYVDTMFGR RRPVPELKSS NYMQRSFGER VAMNSPIQGT
AADIIKIAMI RVNERLKREQ RKARLLLQVH DELLIECPME EVDQVCALLE EEMRGAASLA
VELEVDMHTG KNWYEAK
//
