ID A0A396SEV1_9BACI Unreviewed; 684 AA.
AC A0A396SEV1;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=peptidoglycan glycosyltransferase {ECO:0000256|ARBA:ARBA00012555};
DE EC=2.4.1.129 {ECO:0000256|ARBA:ARBA00012555};
GN ORFNames=D1B33_04135 {ECO:0000313|EMBL:RHW40046.1};
OS Lysinibacillus yapensis.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Lysinibacillus.
OX NCBI_TaxID=2304605 {ECO:0000313|EMBL:RHW40046.1, ECO:0000313|Proteomes:UP000265692};
RN [1] {ECO:0000313|EMBL:RHW40046.1, ECO:0000313|Proteomes:UP000265692}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YLB-03 {ECO:0000313|EMBL:RHW40046.1,
RC ECO:0000313|Proteomes:UP000265692};
RA Yu L.;
RT "Lysinibacillus sp. YLB-03 draft genome sequence.";
RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-
CC Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-
CC (1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-
CC cis-undecaprenyl diphosphate = [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-
CC D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl
CC diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+);
CC Xref=Rhea:RHEA:23708, Rhea:RHEA-COMP:9602, Rhea:RHEA-COMP:9603,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58405, ChEBI:CHEBI:60033,
CC ChEBI:CHEBI:78435; EC=2.4.1.129;
CC Evidence={ECO:0000256|ARBA:ARBA00023988};
CC -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004752}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RHW40046.1}.
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DR EMBL; QWEI01000001; RHW40046.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A396SEV1; -.
DR OrthoDB; 9766909at2; -.
DR Proteomes; UP000265692; Unassembled WGS sequence.
DR GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR GO; GO:0008955; F:peptidoglycan glycosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009002; F:serine-type D-Ala-D-Ala carboxypeptidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.3810.10; Biosynthetic peptidoglycan transglycosylase-like; 1.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR001264; Glyco_trans_51.
DR InterPro; IPR023346; Lysozyme-like_dom_sf.
DR InterPro; IPR036950; PBP_transglycosylase.
DR InterPro; IPR001460; PCN-bd_Tpept.
DR NCBIfam; TIGR02074; PBP_1a_fam; 1.
DR PANTHER; PTHR32282; BINDING PROTEIN TRANSPEPTIDASE, PUTATIVE-RELATED; 1.
DR PANTHER; PTHR32282:SF11; PENICILLIN-BINDING PROTEIN 1B; 1.
DR Pfam; PF00912; Transgly; 1.
DR Pfam; PF00905; Transpeptidase; 1.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR SUPFAM; SSF53955; Lysozyme-like; 1.
PE 4: Predicted;
KW Carboxypeptidase {ECO:0000256|ARBA:ARBA00022645};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000265692}.
FT DOMAIN 66..237
FT /note="Glycosyl transferase family 51"
FT /evidence="ECO:0000259|Pfam:PF00912"
FT DOMAIN 327..572
FT /note="Penicillin-binding protein transpeptidase"
FT /evidence="ECO:0000259|Pfam:PF00905"
SQ SEQUENCE 684 AA; 76506 MW; 5B0F9B11AB35290B CRC64;
MKRQNFRKQR KRKKWLKYTA FTVVAAFSAI IVGLITLRVY AQVAGAPPLT VPKASVFLDN
NGHRIGDYFA AERRYWVELD EISPYLIDAT IAVEDKDFYE HSGFDYSRIA SALLTDLKTQ
SMAEGASTLT MQLARNLYLS HAKTWTRKIQ EAMYAYRLEV FYDKDEILEG YLNTVNYGHG
MYGVEAASRY YFGKSAGDLT LAESALLAGI PKGPSIYSPI NDFEKSIGRQ QVILSLMDSQ
QYISTEEKER ANSEEIVLKN EQWTATKSIA PYFLDVAWQE AAGLLEKENL NIREGGWTIK
TTLNQAHQKA AEEAVADNMP KSDLQVGFVS MDVRTGYVTA LVGGRNYEDS SFNRVTQAVR
QPGSAIKPLL YAAALENGFT PLTYMDVGET IFTYDNGRAT YKPKNVNGQF AEGEMSLAQA
LAISDNIYAV KTLEKIGYKP YQDILERFDL NVAGADNPSA ALGTTENSLF DITSAYNTIA
NEGKKTKPTT ILEIKNGNGE VVYEYKPPEE KQVISEEDAF LLTDMMTGIF DPVFSDYSPA
TGVSLRPRMT HTYAAKSGTT DSDQWIVGYT PSVTAGVWNG YDQGKTLSVQ QDMAATKHVW
IDFMETVNKG KANESFKAPE GVEGVLIDIE TGKLATDACP KQRMMYLKTE DVPTEKCSSD
FFDKESWDNF LDLFPFETFK NFFN
//
