ID   A0A397L1T3_BRACM        Unreviewed;      1051 AA.
AC   A0A397L1T3;
DT   05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT   05-DEC-2018, sequence version 1.
DT   27-MAR-2024, entry version 19.
DE   RecName: Full=tRNA synthetases class I catalytic domain-containing protein {ECO:0000259|Pfam:PF01406};
GN   ORFNames=BRARA_K01109 {ECO:0000313|EMBL:RIA04637.1};
OS   Brassica campestris (Field mustard).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3711 {ECO:0000313|EMBL:RIA04637.1};
RN   [1] {ECO:0000313|EMBL:RIA04637.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Bowman J., Kohchi T., Yamato K., Jenkins J., Shu S., Ishizaki K.,
RA   Yamaoka S., Nishihama R., Nakamura Y., Berger F., Adam C., Aki S.,
RA   Althoff F., Araki T., Arteaga-Vazquez M., Balasubrmanian S., Bauer D.,
RA   Boehm C., Briginshaw L., Caballero-Perez J., Catarino B., Chen F.,
RA   Chiyoda S., Chovatia M., Davies K., Delmans M., Demura T., Dierschke T.,
RA   Dolan L., Dorantes-Acosta A., Eklund D., Florent S., Flores-Sandoval E.,
RA   Fujiyama A., Fukuzawa H., Galik B., Grimanelli D., Grimwood J.,
RA   Grossniklaus U., Hamada T., Haseloff J., Hetherington A., Higo A.,
RA   Hirakawa Y., Hundley H., Ikeda Y., Inoue K., Inoue S., Ishida S., Jia Q.,
RA   Kakita M., Kanazawa T., Kawai Y., Kawashima T., Kennedy M., Kinose K.,
RA   Kinoshita T., Kohara Y., Koide E., Komatsu K., Kopischke S., Kubo M.,
RA   Kyozuka J., Lagercrantz U., Lin S., Lindquist E., Lipzen A., Lu C.,
RA   Luna E., Martienssen R., Minamino N., Mizutani M., Mizutani M.,
RA   Mochizuki N., Monte I., Mosher R., Nagasaki H., Nakagami H., Naramoto S.,
RA   Nishitani K., Ohtani M., Okamoto T., Okumura M., Phillips J., Pollak B.,
RA   Reinders A., Roevekamp M., Sano R., Sawa S., Schmid M., Shirakawa M.,
RA   Solano R., Spunde A., Suetsugu N., Sugano S., Sugiyama A., Sun R.,
RA   Suzuki Y., Takenaka M., Takezawa D., Tomogane H., Tsuzuki M., Ueda T.,
RA   Umeda M., Ward J., Watanabe Y., Yazaki K., Yokoyama R., Yoshitake Y.,
RA   Yotsui I., Zachgo S., Schmutz J.;
RT   "WGS assembly of Brassica rapa FPsc.";
RL   Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
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DR   EMBL; KZ866322; RIA04637.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A397L1T3; -.
DR   Proteomes; UP000264353; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.620; HUPs; 1.
DR   InterPro; IPR024909; Cys-tRNA/MSH_ligase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR032678; tRNA-synt_1_cat_dom.
DR   PANTHER; PTHR10890:SF29; CYSTEINE--TRNA LIGASE; 1.
DR   PANTHER; PTHR10890; CYSTEINYL-TRNA SYNTHETASE; 1.
DR   Pfam; PF01406; tRNA-synt_1e; 1.
DR   PRINTS; PR00983; TRNASYNTHCYS.
DR   SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
PE   4: Predicted;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Ligase {ECO:0000256|ARBA:ARBA00022598};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741}.
FT   DOMAIN          362..603
FT                   /note="tRNA synthetases class I catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF01406"
FT   REGION          105..139
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          776..795
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          633..710
FT                   /evidence="ECO:0000256|SAM:Coils"
FT   COILED          802..877
FT                   /evidence="ECO:0000256|SAM:Coils"
SQ   SEQUENCE   1051 AA;  123519 MW;  604504249C2A043E CRC64;
     MFYGEDPERW VEWIDVLVAA HNFTVFKTRK FMYGFNEGHA LSWYGDEISQ YGFSSWGDLK
     VRLLNRFNTS AKEEKEQLEQ SRFMDILKEI SNRFEQRWKR KEDKEVSGGE DIGKEKRIKK
     SQEEKTLESD DVKQVESDDG GDDLIMYSNQ LIQYVKSDML ILAYPVMVQE KDDPETETLL
     FDEDNSSKTE MDSGACQVFE KILKRRKKVT KKKKRLKKLF SDIEDQLVEF LSHKESDNSD
     MLGIQMENTT KRQRKSWLEW SKGNCHWVHT RQRLDRKWML IFRKKKRKRK REIKVLLGVE
     NKIEIVKSYQ RGEIEELEKL LVKMNDTMES FTKEEKAIEL KLYNTMTQQK EVLNPINLEL
     MINPGKVRMY VCGMTAYDLR HIGHNRAAVF FDVLYRYLKQ LGYEVNYVRI FTDVDDKIIK
     HANENGEKPL DLSKRFCKEY WYLRSHIDDI IIYIDNIIEK GLGYAVKRED VFLSVEKFTD
     YGKLSCQLLE HKWGGEQIEV DPRKRKPAVF TLWKAAKPNW ESSWGQRVKP RWHIESSVVS
     AHYLSPSFVV HGGGADLKFP HHENKLAQTC SVCDDGGMSY WLHNRHVTIH NDKMAKSKKN
     LYEFIEKEKA MVVSVEKQME MFYVQCLDKE KSIGKLSREK IELEEHLLQE EKRRVELIRK
     IDELVRVETS EKLQCKVGEI RMLEHVELER ENLNDALDEE KRHGEDLKAD VSKSEKMIET
     TLEEFEKIRI ERESLSTVKA ILEKKLQDLT NPINALKKEV KSAVMEAKHS LVTLKGTASD
     VSQSDSEQQK QENGTKSYAL DLVSIEKAYE TEEDIIEEMK KEAKSMKLST EEYHKKKKKV
     EKVPCIVSDW PLEEHELKAL EARRARALRE IEHKRRKLGL VEDDNSKTEE GYEHEKKEDN
     VTRVVNGRDD SEKAFYKELF YHSRFHGTLA DIFLRDTDLI SDSRGMVAFV ATITTTLTGY
     LYYITASPKD PTYIMAPVLM LRYARDELKM MAPYIEKDWE VNKCKVKEQK LESRMCSSLR
     GRMLKVSLKK ACGTSYPKSI WNVKEIGNIL V
//