ID   A0A397XNT4_BRACM        Unreviewed;       523 AA.
AC   A0A397XNT4;
DT   05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT   05-DEC-2018, sequence version 1.
DT   27-MAR-2024, entry version 17.
DE   RecName: Full=NEDD8-activating enzyme E1 regulatory subunit {ECO:0000256|PIRNR:PIRNR039099};
GN   ORFNames=BRARA_J00338 {ECO:0000313|EMBL:RID40280.1};
OS   Brassica campestris (Field mustard).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3711 {ECO:0000313|EMBL:RID40280.1, ECO:0000313|Proteomes:UP000264353};
RN   [1] {ECO:0000313|EMBL:RID40280.1, ECO:0000313|Proteomes:UP000264353}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. B-3 {ECO:0000313|Proteomes:UP000264353};
RA   Bowman J., Kohchi T., Yamato K., Jenkins J., Shu S., Ishizaki K.,
RA   Yamaoka S., Nishihama R., Nakamura Y., Berger F., Adam C., Aki S.,
RA   Althoff F., Araki T., Arteaga-Vazquez M., Balasubrmanian S., Bauer D.,
RA   Boehm C., Briginshaw L., Caballero-Perez J., Catarino B., Chen F.,
RA   Chiyoda S., Chovatia M., Davies K., Delmans M., Demura T., Dierschke T.,
RA   Dolan L., Dorantes-Acosta A., Eklund D., Florent S., Flores-Sandoval E.,
RA   Fujiyama A., Fukuzawa H., Galik B., Grimanelli D., Grimwood J.,
RA   Grossniklaus U., Hamada T., Haseloff J., Hetherington A., Higo A.,
RA   Hirakawa Y., Hundley H., Ikeda Y., Inoue K., Inoue S., Ishida S., Jia Q.,
RA   Kakita M., Kanazawa T., Kawai Y., Kawashima T., Kennedy M., Kinose K.,
RA   Kinoshita T., Kohara Y., Koide E., Komatsu K., Kopischke S., Kubo M.,
RA   Kyozuka J., Lagercrantz U., Lin S., Lindquist E., Lipzen A., Lu C.,
RA   Luna E., Martienssen R., Minamino N., Mizutani M., Mizutani M.,
RA   Mochizuki N., Monte I., Mosher R., Nagasaki H., Nakagami H., Naramoto S.,
RA   Nishitani K., Ohtani M., Okamoto T., Okumura M., Phillips J., Pollak B.,
RA   Reinders A., Roevekamp M., Sano R., Sawa S., Schmid M., Shirakawa M.,
RA   Solano R., Spunde A., Suetsugu N., Sugano S., Sugiyama A., Sun R.,
RA   Suzuki Y., Takenaka M., Takezawa D., Tomogane H., Tsuzuki M., Ueda T.,
RA   Umeda M., Ward J., Watanabe Y., Yazaki K., Yokoyama R., Yoshitake Y.,
RA   Yotsui I., Zachgo S., Schmutz J.;
RT   "WGS assembly of Brassica rapa FPsc.";
RL   Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Regulatory subunit of the dimeric E1 enzyme. E1 activates
CC       RUB1/NEDD8 by first adenylating its C-terminal glycine residue with
CC       ATP, thereafter linking this residue to the side chain of the catalytic
CC       cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally
CC       transfers RUB1 to the catalytic cysteine of RCE1.
CC       {ECO:0000256|PIRNR:PIRNR039099}.
CC   -!- PATHWAY: Protein modification; protein neddylation.
CC       {ECO:0000256|ARBA:ARBA00005032, ECO:0000256|PIRNR:PIRNR039099}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-activating E1 family. ULA1
CC       subfamily. {ECO:0000256|ARBA:ARBA00006868,
CC       ECO:0000256|PIRNR:PIRNR039099}.
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DR   EMBL; CM010637; RID40280.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A397XNT4; -.
DR   UniPathway; UPA00885; -.
DR   Proteomes; UP000264353; Chromosome a10.
DR   GO; GO:0019781; F:NEDD8 activating enzyme activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0045116; P:protein neddylation; IEA:UniProtKB-UniRule.
DR   CDD; cd01493; APPBP1_RUB; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 2.
DR   InterPro; IPR030667; APP-BP1.
DR   InterPro; IPR045886; ThiF/MoeB/HesA.
DR   InterPro; IPR000594; ThiF_NAD_FAD-bd.
DR   InterPro; IPR035985; Ubiquitin-activating_enz.
DR   PANTHER; PTHR10953:SF247; NEDD8-ACTIVATING ENZYME E1 REGULATORY SUBUNIT; 1.
DR   PANTHER; PTHR10953; UBIQUITIN-ACTIVATING ENZYME E1; 1.
DR   Pfam; PF00899; ThiF; 1.
DR   PIRSF; PIRSF039099; APP-BP1; 1.
DR   SUPFAM; SSF69572; Activating enzymes of the ubiquitin-like proteins; 1.
PE   3: Inferred from homology;
KW   Ubl conjugation pathway {ECO:0000256|PIRNR:PIRNR039099}.
FT   DOMAIN          8..501
FT                   /note="THIF-type NAD/FAD binding fold"
FT                   /evidence="ECO:0000259|Pfam:PF00899"
SQ   SEQUENCE   523 AA;  57933 MW;  6C626BC424115318 CRC64;
     MSEAKTKYDR QLRIWGEVGQ AALEEASICL LNCGPTGSEA LKNLVLGGVG SITVVDGSKV
     ELGDLGNNFM VDEGSVGQSK AKSVCAFLQE LNDSVKAKFI EEDPDTLIMT NPSFFSHFTL
     VIATQLVEES MVKLDRICRE ANVKLVFARS YGLAGFVRVS VKEHTIIDSK PDHFLDDLRL
     NNPWPELKSF VETIDLNVSD PVAHKHIPYV VILVKMADEW TQSHSGNLPS TREEKKEFKD
     LVKSKMISMD EDNYKEAIEA AFKVFAPRGI SSEIQQISSD TCAEPSSNSS DFWVMVAALK
     EFVSNEGDGE APLEGSIPDM TSSTEHYINL QKIYLAKAES DFLVMEERVK NILKKIGRDP
     SSISKPTIKS FCKNARKLKV CRYRMVEDEF SNPSVTEIQK CLADEDYSGA MGFYILLRAV
     DRFTANYNKF PGQFDGGMDE DISRLKTTAL SLLTDLGCNG SVLPDDLINE MCRFGASELH
     VVAAFLGGIA SQEAIKLVTK QFVPMLGTYI FNGIDHKSQL LAL
//