ID A0A397YWV2_BRACM Unreviewed; 2174 AA.
AC A0A397YWV2;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=DNA polymerase epsilon catalytic subunit {ECO:0000256|RuleBase:RU365029};
DE EC=2.7.7.7 {ECO:0000256|RuleBase:RU365029};
GN ORFNames=BRARA_F00511 {ECO:0000313|EMBL:RID57108.1};
OS Brassica campestris (Field mustard).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3711 {ECO:0000313|EMBL:RID57108.1, ECO:0000313|Proteomes:UP000264353};
RN [1] {ECO:0000313|EMBL:RID57108.1, ECO:0000313|Proteomes:UP000264353}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. B-3 {ECO:0000313|Proteomes:UP000264353};
RA Bowman J., Kohchi T., Yamato K., Jenkins J., Shu S., Ishizaki K.,
RA Yamaoka S., Nishihama R., Nakamura Y., Berger F., Adam C., Aki S.,
RA Althoff F., Araki T., Arteaga-Vazquez M., Balasubrmanian S., Bauer D.,
RA Boehm C., Briginshaw L., Caballero-Perez J., Catarino B., Chen F.,
RA Chiyoda S., Chovatia M., Davies K., Delmans M., Demura T., Dierschke T.,
RA Dolan L., Dorantes-Acosta A., Eklund D., Florent S., Flores-Sandoval E.,
RA Fujiyama A., Fukuzawa H., Galik B., Grimanelli D., Grimwood J.,
RA Grossniklaus U., Hamada T., Haseloff J., Hetherington A., Higo A.,
RA Hirakawa Y., Hundley H., Ikeda Y., Inoue K., Inoue S., Ishida S., Jia Q.,
RA Kakita M., Kanazawa T., Kawai Y., Kawashima T., Kennedy M., Kinose K.,
RA Kinoshita T., Kohara Y., Koide E., Komatsu K., Kopischke S., Kubo M.,
RA Kyozuka J., Lagercrantz U., Lin S., Lindquist E., Lipzen A., Lu C.,
RA Luna E., Martienssen R., Minamino N., Mizutani M., Mizutani M.,
RA Mochizuki N., Monte I., Mosher R., Nagasaki H., Nakagami H., Naramoto S.,
RA Nishitani K., Ohtani M., Okamoto T., Okumura M., Phillips J., Pollak B.,
RA Reinders A., Roevekamp M., Sano R., Sawa S., Schmid M., Shirakawa M.,
RA Solano R., Spunde A., Suetsugu N., Sugano S., Sugiyama A., Sun R.,
RA Suzuki Y., Takenaka M., Takezawa D., Tomogane H., Tsuzuki M., Ueda T.,
RA Umeda M., Ward J., Watanabe Y., Yazaki K., Yokoyama R., Yoshitake Y.,
RA Yotsui I., Zachgo S., Schmutz J.;
RT "WGS assembly of Brassica rapa FPsc.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA polymerase II participates in chromosomal DNA
CC replication. {ECO:0000256|RuleBase:RU365029}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|RuleBase:RU365029};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966,
CC ECO:0000256|RuleBase:RU365029};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|RuleBase:RU365029}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family.
CC {ECO:0000256|ARBA:ARBA00005755, ECO:0000256|RuleBase:RU365029}.
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DR EMBL; CM010633; RID57108.1; -; Genomic_DNA.
DR Proteomes; UP000264353; Chromosome a6.
DR GO; GO:0008622; C:epsilon DNA polymerase complex; IEA:InterPro.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd05779; DNA_polB_epsilon_exo; 1.
DR CDD; cd05535; POLBc_epsilon; 1.
DR Gene3D; 1.10.132.60; DNA polymerase family B, C-terminal domain; 1.
DR Gene3D; 3.30.342.10; DNA Polymerase, chain B, domain 1; 1.
DR Gene3D; 3.90.1600.10; Palm domain of DNA polymerase; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR042087; DNA_pol_B_thumb.
DR InterPro; IPR013697; DNA_pol_e_suA_C.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR029703; POL2.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR10670:SF0; DNA POLYMERASE EPSILON CATALYTIC SUBUNIT 1; 1.
DR PANTHER; PTHR10670; DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A; 1.
DR Pfam; PF00136; DNA_pol_B; 1.
DR Pfam; PF03104; DNA_pol_B_exo1; 1.
DR Pfam; PF08490; DUF1744; 1.
DR SMART; SM01159; DUF1744; 1.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485, ECO:0000256|RuleBase:RU365029};
KW DNA replication {ECO:0000256|RuleBase:RU365029};
KW DNA-binding {ECO:0000256|RuleBase:RU365029};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU365029};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU365029};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|RuleBase:RU365029};
KW Metal-binding {ECO:0000256|RuleBase:RU365029};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU365029};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU365029};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU365029};
KW Zinc {ECO:0000256|RuleBase:RU365029};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|RuleBase:RU365029}.
FT DOMAIN 1490..1874
FT /note="DNA polymerase epsilon catalytic subunit A C-
FT terminal"
FT /evidence="ECO:0000259|SMART:SM01159"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2174 AA; 250260 MW; 98E517F5692F0ED4 CRC64;
MSGDNRRWDR KDSRRSNTQK VVKTAEDELE SKLGFGLFSE GETRLGWLLT FASSSWEDPD
TGKTYSCVDL YFVTQDGFYF KTKYKFRPYF YAATKEKMEL EVEAYLRRRY ERQVADIEIV
EKEDLDLKNH LSGLRKKYLK VSFDTVQQLM EVKRDLSHVV ERNQAKFDAL EAYESILAGK
REQRLQDCLD SLVELREYDV PYHVRFAVDK DVRSGQWYNV SISSTDVTLE KRTDILQRAE
VRVCAFDIET TKLELKFPDA EYDQIMMISY MVDGQGFLIV NRECVGEDIE DLEYTPKPEF
EGYFKVTNVS NEVELLRKWF SHMQEVKPGI YVTYNGDFFD WPFIERRASH HEIKMNEELG
FRCDHNQGEC RAKFVCHLDC FAWVKRDSYL PQGSHGLKAV TKAKLGYDPL EVNPEDMVQF
AMEKPQTMAS YSVSDAVATY YLYMTYVHPF IFSLATIIPM VPDEVLRKGS GTLCEMLLMV
EAYKANVVCP NKNQADPEKF YQSHLLESET YIGGHVECLE SGVFRSDIPT SFKLDTSAYQ
QLIDNLDRDL EYAITVEGKM RMDSVSNYDE VKEKIKEKLE KLRDCPIREE GPLIYHLDVA
AMYPNIILTN RLQPPSIVTN EICTACDFNR PGKTCLRKLE WVWRGVTFMA KKSDYYHLKK
QIESEFVDAG TNIQSSKSFL DLPKVEQQSK LKERLKKYCQ KAYRRVLDKP STEVREAGIC
MRENPFYVDT VRSFRDRRYE YKTLNKVWKG KLSEAKASGN SIKIQEAQDM VVVYDSLQLA
HKCILNSFYG YVMRKGARWY SMEMAGVVTY TGAKIIQNAC LLIERIGRPL ELDTDGIWCA
LPGSFPENFT FKTIDMKKFT ISYPCVMLNV DVAKNNTNDQ YQTLVDPVRK TYESHSECSI
EFEVDGPYKA MIIPASKEEG VLLKKRYAVF NHDGTLAELK GFEIKRRGEL KLIKVFQAEL
FDKFLHGSTL EECYSAVAAV ANRWLDLLDN QGKDIADSEL LDYISESSTM SKSLADYGEQ
KSCAVTTAKR LADFLGDTMV KDKGLRCQYI VACEPKGSPV SERAVPVAIF TLSKTEPERM
KFYLGKWCKQ RLSSDHKVDI RLIIDWSYYK QRLSSAIQKV ITIPAAMQKV ANPVPRVLHP
DWLHKKVREK EDKFRQRKLA DMFSSANKNH SVAQDDMNDI EDFCKENKPG VKGPKPIARS
YEVNKEQFGR EQQESKDPKC DDDISFENID KNVDYQGWLE VKKRKWKGIV EKKKKRRLGD
QRSLKQIESH EIKKKVTQVR RGVGSFFRRP EEALTSSHWQ IIQLVPSPQI GQFFAWVVVE
GLMLKIPLTI PRVFYINSKA PIDGKFPGKC VNKILPHGKP CYNMIEVEIQ EEQFRKESKK
LAALLADPEM EGIYETKVPL EFSAICQIGC VCKIDNTAKH RNTQDGWKVG ELHMKTTTEC
RYLERSIPLL YLYNSTSTLR AIYVLYCHAS KLMSAVVVNP YGDKELLPSA LERQFRDGCQ
ELSLESLSWD GIVFQVDYVD HAEAAEKIIQ KAISEYREEN SGPTVVVTEC PDFNSIKDGI
KALDDFPCVR IPFNGDDNSY QPVSWQRPAA KLAMFRCAAA FQWLDRRISQ SRYAHVPLGN
FGLDWLTFTV DIFLSRALRD QQQVLWVSDN GIPDLGGLNN EETFFADEVQ QTSLAYPGAY
RKISVELKIH HLAVNALLKS NLVSEMEGGD FLGFEQDVNP RGSSSNVNAS FDETTGCAQA
FRVLKQLINS CLTDVCKSRN IYADSILQRL SWWLCSPCSK LHDPALHLML HKVMQKVFAL
LLTDLRRLGA TIIFADFSKI VIDTGKFDLS AAKAYCDSLL TAVGNSDIFE WILLEPVHYW
HSLLFMDQYN YAGIQATDDE ISPDEVTIEP KWSVARLFPE YIQRDFVIIV AKFIFDPWKF
SLEKKRGSSE SLEAQMIEYL KEQIPSSFFN MLVAKINDIV SHMKEIDVSD ASRDSSQAPK
RDYTLELIQT ICAVMGLDQN VEQDVRVMKR NLLKYINVKE FAAEAEFLDP GPSFILPNVA
CSNCDAYRDL DIGRDPALLT EKEWCCVDSQ CGKIYDREQM ENSLLQMVRQ RERMYHMQDL
VCTRCNQVKA AHLTEQCECS GSFRCKESGS EFLKRMEIFL DIAKRQKFKL LEECTSWILD
PTGSWQTNTG RHHS
//
