ID A0A397Z8G1_BRACM Unreviewed; 1397 AA.
AC A0A397Z8G1;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU363044};
DE EC=3.6.4.12 {ECO:0000256|RuleBase:RU363044};
GN ORFNames=BRARA_E01052 {ECO:0000313|EMBL:RID61949.1};
OS Brassica campestris (Field mustard).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3711 {ECO:0000313|EMBL:RID61949.1, ECO:0000313|Proteomes:UP000264353};
RN [1] {ECO:0000313|EMBL:RID61949.1, ECO:0000313|Proteomes:UP000264353}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. B-3 {ECO:0000313|Proteomes:UP000264353};
RA Bowman J., Kohchi T., Yamato K., Jenkins J., Shu S., Ishizaki K.,
RA Yamaoka S., Nishihama R., Nakamura Y., Berger F., Adam C., Aki S.,
RA Althoff F., Araki T., Arteaga-Vazquez M., Balasubrmanian S., Bauer D.,
RA Boehm C., Briginshaw L., Caballero-Perez J., Catarino B., Chen F.,
RA Chiyoda S., Chovatia M., Davies K., Delmans M., Demura T., Dierschke T.,
RA Dolan L., Dorantes-Acosta A., Eklund D., Florent S., Flores-Sandoval E.,
RA Fujiyama A., Fukuzawa H., Galik B., Grimanelli D., Grimwood J.,
RA Grossniklaus U., Hamada T., Haseloff J., Hetherington A., Higo A.,
RA Hirakawa Y., Hundley H., Ikeda Y., Inoue K., Inoue S., Ishida S., Jia Q.,
RA Kakita M., Kanazawa T., Kawai Y., Kawashima T., Kennedy M., Kinose K.,
RA Kinoshita T., Kohara Y., Koide E., Komatsu K., Kopischke S., Kubo M.,
RA Kyozuka J., Lagercrantz U., Lin S., Lindquist E., Lipzen A., Lu C.,
RA Luna E., Martienssen R., Minamino N., Mizutani M., Mizutani M.,
RA Mochizuki N., Monte I., Mosher R., Nagasaki H., Nakagami H., Naramoto S.,
RA Nishitani K., Ohtani M., Okamoto T., Okumura M., Phillips J., Pollak B.,
RA Reinders A., Roevekamp M., Sano R., Sawa S., Schmid M., Shirakawa M.,
RA Solano R., Spunde A., Suetsugu N., Sugano S., Sugiyama A., Sun R.,
RA Suzuki Y., Takenaka M., Takezawa D., Tomogane H., Tsuzuki M., Ueda T.,
RA Umeda M., Ward J., Watanabe Y., Yazaki K., Yokoyama R., Yoshitake Y.,
RA Yotsui I., Zachgo S., Schmutz J.;
RT "WGS assembly of Brassica rapa FPsc.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- SIMILARITY: Belongs to the helicase family.
CC {ECO:0000256|RuleBase:RU363044}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CM010632; RID61949.1; -; Genomic_DNA.
DR Proteomes; UP000264353; Chromosome a5.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR CDD; cd18809; SF1_C_RecD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR010285; DNA_helicase_pif1-like.
DR InterPro; IPR025476; Helitron_helicase-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR049163; Pif1-like_2B_dom.
DR PANTHER; PTHR45786; DNA BINDING PROTEIN-LIKE; 1.
DR PANTHER; PTHR45786:SF66; REPLICATION FACTOR-A CARBOXY-TERMINAL DOMAIN PROTEIN; 1.
DR Pfam; PF14214; Helitron_like_N; 1.
DR Pfam; PF05970; PIF1; 1.
DR Pfam; PF21530; Pif1_2B_dom; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU363044};
KW DNA damage {ECO:0000256|RuleBase:RU363044};
KW DNA recombination {ECO:0000256|RuleBase:RU363044};
KW DNA repair {ECO:0000256|RuleBase:RU363044};
KW Helicase {ECO:0000256|RuleBase:RU363044};
KW Hydrolase {ECO:0000256|RuleBase:RU363044};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU363044}.
FT DOMAIN 255..437
FT /note="Helitron helicase-like"
FT /evidence="ECO:0000259|Pfam:PF14214"
FT DOMAIN 1229..1275
FT /note="DNA helicase Pif1-like 2B"
FT /evidence="ECO:0000259|Pfam:PF21530"
FT REGION 581..611
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 581..606
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1397 AA; 158812 MW; D9782795E5E66947 CRC64;
MCCMRGKVKL PLLKEPPALL QRLLTSDDAV SKHYRDNIRP INMMFSFTSL GGKIDNSINA
VQGPKIFKLH GENYHLIGSM KPNQNEKAKF SQLYIHDTQN EVDNRISALS GNSGKSRIRP
DVVASIMEML RECNVHVKTF RNAMDRFNND EETEEVSLVL IHSRLKDGRV YNLPTSSEVA
ALVVGDFQQN MDKRDIILEK TSGKLKRISE LHPCYLPLQY PLIFPYGEDG FRLGIKHGFT
GPTKNKKPNI TMREYFAYRI QVRNVGSQVL HLSRRLLQQF LVDAYTMIES HRLRFIRKNQ
SNLRTLKFSK FVEAANQGQA TLAIEGNRII IPSSFTGGPR YMHQMYLDAM TICKYFGFPD
LFITFTCNPK WPELNRYFEK YKLKSEDRPE LCCRLFKVKL DSLMDDLTKK HLLGKTVSAI
YTIEFQKRGL PHAHILLFMD SKHKLPQADD IDGIISAEIP DKASEPRLYE VVKDLMIHGP
CGVVNKNSPC MQDGRCSKFF PRKNVEKTTV DSQGYPVYRR RDNGAFIEKK GIQCDNTFVV
PYNKKLLLAY NAHINVEWCN QSRSIKYLFK YINKGQDRVT GTVTQKNGNQ PQGESNGRQT
AGPQNVDGNA AEDPQVDEIK NYFDARYIST CESAWRILAF PTQYRSTPVE KLTFHLEGEQ
AVIYKEGDSV KSVMDRVQST KTMFLAWFDC CIIYPEAREL TYAELPTRFV YDGKQKAWNP
RKKGFSIGRL TPVSPSSGEY YFLRVLLNKV RGPTCYEDIK TVKSIVQSSF EDACYELGLL
DDDKEYIEGL KECSFWASSG YVRNLFAKML LSGCLSTPKL VWEATKDLLS EDVLYNERKR
RRYPGLILSE EEILNCTLIM IENILRSKNS SLNNWETMPK PVDNLNSFSE NLLLQEELNY
PKDELRLQHD EWIGKLTDEQ RSVYDQIIGA VLNKKGGVFF VYGFGGTGKT FLWNILSAAI
RSQGEIVLNV ASSGIASLLL PGGRTAHSRF GIPINPDEFS TCKIQPGSNQ AELIARSSLI
IWDEAPMMSK HCFEALDRTM ADIMKSGEGT PFGGKVVVFG GDFRQILPVI PRGNRADIVM
ATLNSSFLWK HCKVLELTKN MRLMAETDLR SAEEIKLFSK WILDLGDGKI NEPNSGETMI
EIPKDLLIKE CNDPIQAIVS EVYGNTFKDS KDPLFFQERA ILCPTNADVE VINNYMLDRL
TGEERVYLSS DSIDPSDLNS KDDSVFSPEF LNSIKVSGLP NHALRLRIGT PVMILRNIDP
TEGLCNGTRL QITQLANHII GAKFITGTRV GEKVFLYRLL IEPSDASLPF KMRRRQFPLK
VAFAMTINKS QGQTLGNVGL YLPKPVFSHG QLYVAVSRVK SRKGLKILIT DKDGKNQDST
MNVVYKEVFQ NLFDSDM
//
