ID   A0A398BH03_9BACI        Unreviewed;       571 AA.
AC   A0A398BH03;
DT   05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT   05-DEC-2018, sequence version 1.
DT   27-MAR-2024, entry version 13.
DE   RecName: Full=Rqc2 homolog RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
DE            Short=RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN   Name=rqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN   ORFNames=D1970_03640 {ECO:0000313|EMBL:RID87938.1};
OS   Mesobacillus zeae.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Mesobacillus.
OX   NCBI_TaxID=1917180 {ECO:0000313|EMBL:RID87938.1, ECO:0000313|Proteomes:UP000265816};
RN   [1] {ECO:0000313|EMBL:RID87938.1, ECO:0000313|Proteomes:UP000265816}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JJ-247 {ECO:0000313|EMBL:RID87938.1,
RC   ECO:0000313|Proteomes:UP000265816};
RA   Seuylemezian A., Vaishampayan P.;
RT   "Bacillus jemisoniae sp. nov., Bacillus chryseoplanitiae sp. nov., Bacillus
RT   resnikiae sp. nov., and Bacillus frankliniae sp. nov., isolated from Viking
RT   spacecraft and associated surfaces.";
RL   Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Part of the ribosome quality control system (RQC). Recruits
CC       Ala-charged tRNA and directs the elongation of stalled nascent chains
CC       on 50S ribosomal subunits, leading to non-templated C-terminal Ala
CC       extensions (Ala tail). The Ala tail promotes nascent chain degradation.
CC       May add between 1 and at least 8 Ala residues. Binds to stalled 50S
CC       ribosomal subunits. {ECO:0000256|HAMAP-Rule:MF_00844}.
CC   -!- SUBUNIT: Associates with stalled 50S ribosomal subunits.
CC       {ECO:0000256|HAMAP-Rule:MF_00844}.
CC   -!- SIMILARITY: Belongs to the NEMF family. {ECO:0000256|HAMAP-
CC       Rule:MF_00844}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RID87938.1}.
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DR   EMBL; QWVT01000008; RID87938.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A398BH03; -.
DR   OrthoDB; 9766163at2; -.
DR   Proteomes; UP000265816; Unassembled WGS sequence.
DR   GO; GO:0043023; F:ribosomal large subunit binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0072344; P:rescue of stalled ribosome; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.8.50; -; 1.
DR   Gene3D; 3.40.970.40; fibrinogen binding protein from staphylococcus aureus domain like; 1.
DR   Gene3D; 2.30.310.10; ibrinogen binding protein from staphylococcus aureus domain; 1.
DR   HAMAP; MF_00844_B; RqcH_B; 1.
DR   InterPro; IPR008532; NFACT_RNA-bd.
DR   InterPro; IPR043682; RqcH_bacterial.
DR   PANTHER; PTHR15239; NUCLEAR EXPORT MEDIATOR FACTOR NEMF; 1.
DR   PANTHER; PTHR15239:SF6; RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF; 1.
DR   Pfam; PF05670; NFACT-R_1; 1.
DR   Pfam; PF18297; NFACT-R_2; 1.
DR   Pfam; PF05833; NFACT_N; 1.
PE   3: Inferred from homology;
KW   Coiled coil {ECO:0000256|HAMAP-Rule:MF_00844};
KW   Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW   Rule:MF_00844}; Reference proteome {ECO:0000313|Proteomes:UP000265816};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|HAMAP-
KW   Rule:MF_00844};
KW   tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|HAMAP-
KW   Rule:MF_00844}.
FT   DOMAIN          453..541
FT                   /note="NFACT RNA-binding"
FT                   /evidence="ECO:0000259|Pfam:PF05670"
FT   COILED          380..407
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00844"
SQ   SEQUENCE   571 AA;  64785 MW;  803C0F6E1B9EF86B CRC64;
     MSFDGLFTRA VTNQLSSLLK GGRINKIHQP YKNEIIMTVR AGGVNHKLLL SAHPSYARVQ
     LANGQYDNPD TPPMFCMLLR KHLEGNIIEN IRQYGLDRII IFEIKGRNEL GDLSYKQLIV
     EIMGRHSNIV LVDKERNMIL DSIKHVSFAV NTHRAIQPGQ EYILPPAQDK INPFEASQED
     VLRKVDFNAG KIDQQLVANF AGLSPLVSRE IVYRAGLANR GTIPNAFTEM MRKLESHEYE
     PAFTSSQGKE SFYLVPLTHM EGETRTFSSL GALLDRFYFG KAERDRVKQQ GNDLEKFIVN
     ERAKNENKIK KLEQTLSEAR NAGQLQLFGE LLTANLHLAK KGMKALEVIN YYDENGASIT
     IPLDPLKTPS ENAQKYFTKY QKAKNAVKIV EEQIEKAKEE AAYFEMLYQQ VESASPKDIA
     EIREELAEGG YIRQRQKRGN KRGQPSKITL EKYISSDGTE ILVGKNNKQN DYLTNKAAAR
     DDIWLHTKDI PGSHVVIRNS EPSEKTILEA SCLAAYYSKA RQSSSVPVDY TRIRYVKKPA
     GSKPGFVIYD NQQTVYITPD EEKVLTMKTD R
//