ID A0A398CJN5_9BACL Unreviewed; 891 AA.
AC A0A398CJN5;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=D3H35_20080 {ECO:0000313|EMBL:RIE02913.1};
OS Cohnella faecalis.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Cohnella.
OX NCBI_TaxID=2315694 {ECO:0000313|EMBL:RIE02913.1, ECO:0000313|Proteomes:UP000266340};
RN [1] {ECO:0000313|EMBL:RIE02913.1, ECO:0000313|Proteomes:UP000266340}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K2E09-144 {ECO:0000313|EMBL:RIE02913.1,
RC ECO:0000313|Proteomes:UP000266340};
RA Zhu H.;
RT "Cohnella cavernae sp. nov., isolated from a karst cave.";
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SUBUNIT: Single-chain monomer with multiple functions.
CC {ECO:0000256|ARBA:ARBA00011541, ECO:0000256|RuleBase:RU004460}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RIE02913.1}.
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DR EMBL; QXJM01000039; RIE02913.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A398CJN5; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000266340; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06140; DNA_polA_I_Bacillus_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000266340};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 6..264
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 307..481
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 648..855
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 891 AA; 98252 MW; 5B9C49B227933D86 CRC64;
MGNGTDKLVL IDGNSIINRA FYAIPPLTNG SGLHTNAVLG FTTMLLKVLE EEKPSHILVA
FDAGKVTFRH EGFAEYKGGR QKTPPELSEQ FPLLKELLPA FGISQFELAG YEADDIIGTL
SRVAEEKGGS VVIVSGDKDM LQLASDQVTV LITRKGVSEV ERFTPEDVNE KYGLTPAQII
DLKGLMGDAS DNIPGVPGVG EKTALKLLHE YGTVENVLEK VTELKGKLRE NIENNMDSAR
MSKQVATIFR EVPLEKTLDD AAYSGYQSAE LVGALRKLEF KSLIERLDLV GAVSEDAGAA
GSEPLAYEIV LVEEAGWGEL NDALAANVDS LIVESNGDNP HQAEGIGLAV AAGEKIYFVP
WELLTAADAG PLRAWLGELS AAKSGYDLHR DELVLARAGI ELRGLAFDAL LAAYVLDPTE
ADPSIGGLLS RHGLASIAPD EAVFGKGAKF RVPDRERLAK HLAAKADAVR RLKPLLVEEM
EKQSMHRLYY EVEQPLSVVL AGMEKQGISV NGEALQELGA EFQRRIEEIV SAIYKIAGFE
FNIGSPKQLG EVLFDKLGLP VVKKTKTGYS TDAEVLEKLE PYHEIVRQIL LYRQLAKLQS
TYIEGLLKEI RPETGKIHTF YRQTIAATGR LSSQFPNLQN IPIRLEEGRQ IRKAFVPSEP
GWRILAADYS QIELRVLAHI SGDEGLKEAF RNDMDIHTKT ASDVFGVSPD QVDANMRRSA
KAVNFGIVYG ISDFGLSQNL NITRKEAAAF IEQYFSVFQG VSRFMETIKQ QAKQDGYVST
LLDRRRYLPD IRASNFNLRS FAERTAMNSP IQGTAADIIK LAMLRMDEAL RERGLRSRML
LQVHDELVFE VPEEELELMS KLVPEVMESA LKLDVPLKAD VSHGSNWYEA K
//
