ID A0A399D0Y1_9BACT Unreviewed; 359 AA.
AC A0A399D0Y1;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE SubName: Full=[FeFe] hydrogenase H-cluster radical SAM maturase HydE {ECO:0000313|EMBL:RIH65083.1};
GN Name=hydE {ECO:0000313|EMBL:RIH65083.1};
GN ORFNames=D1164_10875 {ECO:0000313|EMBL:RIH65083.1};
OS Mariniphaga sediminis.
OC Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales; Prolixibacteraceae;
OC Mariniphaga.
OX NCBI_TaxID=1628158 {ECO:0000313|EMBL:RIH65083.1, ECO:0000313|Proteomes:UP000266441};
RN [1] {ECO:0000313|EMBL:RIH65083.1, ECO:0000313|Proteomes:UP000266441}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SY21 {ECO:0000313|EMBL:RIH65083.1,
RC ECO:0000313|Proteomes:UP000266441};
RX PubMed=26012581; DOI=10.1099/ijs.0.000354;
RA Wang F.Q., Shen Q.Y., Chen G.J., Du Z.J.;
RT "Mariniphaga sediminis sp. nov., isolated from coastal sediment.";
RL Int. J. Syst. Evol. Microbiol. 65:2908-2912(2015).
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC Evidence={ECO:0000256|ARBA:ARBA00034078};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RIH65083.1}.
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DR EMBL; QWET01000007; RIH65083.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A399D0Y1; -.
DR OrthoDB; 9775764at2; -.
DR Proteomes; UP000266441; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0044271; P:cellular nitrogen compound biosynthetic process; IEA:UniProt.
DR GO; GO:0018130; P:heterocycle biosynthetic process; IEA:UniProt.
DR GO; GO:1901362; P:organic cyclic compound biosynthetic process; IEA:UniProt.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR GO; GO:0044272; P:sulfur compound biosynthetic process; IEA:UniProt.
DR GO; GO:0042364; P:water-soluble vitamin biosynthetic process; IEA:UniProt.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR010722; BATS_dom.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR024021; FeFe-hyd_HydE_rSAM.
DR InterPro; IPR034422; HydE/PylB-like.
DR InterPro; IPR007197; rSAM.
DR NCBIfam; TIGR03956; rSAM_HydE; 1.
DR PANTHER; PTHR43726; 3-METHYLORNITHINE SYNTHASE; 1.
DR PANTHER; PTHR43726:SF1; BIOTIN SYNTHASE; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR PIRSF; PIRSF004762; CHP00423; 1.
DR SFLD; SFLDG01060; BATS_domain_containing; 1.
DR SFLD; SFLDG01280; HydE/PylB-like; 1.
DR SMART; SM00876; BATS; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000266441};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 46..272
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 359 AA; 41187 MW; 95C66F3952C04DCD CRC64;
MKTVTEIVNQ QLFSAEDLRV LLTAKGRDRE LLFAKAREVK ESVTGKKVYF RGLVEFSNVC
SKDCLYCGIR KSNGKVVRYN ATDEEILEAC RFAWEKRFGS VVLQSGELES KSFVQRVDKL
VKKIKQLSNN ELGITLSCGE QSQETYLRWF ESGAHRYLLR IEASNRELYY KIHPDTPKHS
FEKRVEALQF LREAGYQLGT GVMIGLPFQT SDDLVNDLLF FKKIDIDMCG MGPYIEHEHT
PLYQFRHLLQ PKMDRFHLAL NMIAVLRLLM PDINIAAATA LQAIDPAGRE KALTVGANVI
MPNLTPCEYR KEYLLYEDKP CLDEDAALCR NCLEARIELA GSEIGYDEWG DSKHFFSRQ
//
