ID A0A3A1QVU8_9BACI Unreviewed; 744 AA.
AC A0A3A1QVU8;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=D3H55_13345 {ECO:0000313|EMBL:RIW32256.1};
OS Bacillus salacetis.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=2315464 {ECO:0000313|EMBL:RIW32256.1, ECO:0000313|Proteomes:UP000265801};
RN [1] {ECO:0000313|EMBL:RIW32256.1, ECO:0000313|Proteomes:UP000265801}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SKP7-4 {ECO:0000313|EMBL:RIW32256.1,
RC ECO:0000313|Proteomes:UP000265801};
RA Daroonpunt R., Tanasupawat S., Yiamsombut S.;
RT "Bacillus saliacetes sp. nov., isolated from Thai shrimp paste (Ka-pi).";
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RIW32256.1}.
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DR EMBL; QXIR01000018; RIW32256.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3A1QVU8; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000265801; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR InterPro; IPR025916; YdjO.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR Pfam; PF14169; YdjO; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000265801}.
FT DOMAIN 48..322
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 323..596
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 69..76
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 744 AA; 85534 MW; A8B144FD75619729 CRC64;
MNNFSNRPFG VSSQVTPNAA VYHREDQFKQ DGESEDDSYF LLLEREGICL NDAQMKAVRH
TQGPCLTLAG AGSGKTSVLV SRTGYLLQVK GISPDNILLL TFSKKAAMEM KHRVAVLPGV
DHKEVNRIQA GTFHSFFLYI LRSRGFKQDI LGNERFKHIQ LKQIQRKLGI QEPYQPETLL
STLSNLKMNM KTLEDLPQKT TGDKEIKKIL LMFEEWKERN EKMDFDDILV KAHELLTQDH
RLLNALQNRF LHVMVDEFQD TNRLQYELIK LISHRSRNLF VVGDDDQTIY SFNGADHSFI
LDFDKEYPDA IVITLDINYR SNSYIVGLGN QVISRNRHRR KKTLKVVKEK PAKPQYSRPS
NSDEEAEWIV AKAKDLVSNG KMEYKDIAIL HRTASSSRAI FEKMVMDEIP FFSYNLGDQH
FYDHWVVKPL IDHLRLSLVP RNFAAIEGIL GTLFINRAAG MKFIFSHEES SRKKYPLIHL
SKMEQLHEFQ REKVKDRVRM IKNLSAEPPA AAIKQMRKDY YDHYMDTNEQ VSVTEHKEVI
KETLSELEAS AARFQSISAF ISFIDSITEK HEKMQQEDHK SNADAISLMT IHRAKGLEFP
AVFVIGASEG NLPHTSALNA DKLEDKIHLD TRNKNILAIE EERRLMYVAI TRAKEALFIS
SPAFYQGEKR DISRFLTDAF YEEPAKKEVA ANKALPKIKV AAWICSNEGC IAWSRITEKE
QNLKRKICPL CETRMAKGEK LVSG
//
