ID   A0A3A1UUJ2_9BACL        Unreviewed;       543 AA.
AC   A0A3A1UUJ2;
DT   05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT   05-DEC-2018, sequence version 1.
DT   24-JAN-2024, entry version 12.
DE   RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE            EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN   ORFNames=D3P08_16135 {ECO:0000313|EMBL:RIX51446.1};
OS   Paenibacillus nanensis.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX   NCBI_TaxID=393251 {ECO:0000313|EMBL:RIX51446.1, ECO:0000313|Proteomes:UP000266482};
RN   [1] {ECO:0000313|EMBL:RIX51446.1, ECO:0000313|Proteomes:UP000266482}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 22867 {ECO:0000313|EMBL:RIX51446.1,
RC   ECO:0000313|Proteomes:UP000266482};
RA   Jurado V., Gutierrez-Patricio S., Gonzalez-Pimentel J.L., Miller A.Z.,
RA   Laiz L., Saiz-Jimenez C.;
RT   "Paenibacillus aracenensis nov. sp. isolated from a cave in southern
RT   Spain.";
RL   Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC         Evidence={ECO:0000256|RuleBase:RU361166};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000256|PROSITE-ProRule:PRU10060, ECO:0000256|RuleBase:RU361166}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RIX51446.1}.
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DR   EMBL; QXQA01000010; RIX51446.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A3A1UUJ2; -.
DR   OrthoDB; 9758662at2; -.
DR   Proteomes; UP000266482; Unassembled WGS sequence.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   CDD; cd02850; E_set_Cellulase_N; 1.
DR   Gene3D; 1.50.10.10; -; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR004197; Cellulase_Ig-like.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR   PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR   Pfam; PF02927; CelD_N; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS00698; GH9_3; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW   ECO:0000256|PROSITE-ProRule:PRU10060};
KW   Cellulose degradation {ECO:0000256|RuleBase:RU361166};
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW   ProRule:PRU10060};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU10060};
KW   Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW   ECO:0000256|PROSITE-ProRule:PRU10060};
KW   Reference proteome {ECO:0000313|Proteomes:UP000266482}.
FT   DOMAIN          6..82
FT                   /note="Cellulase Ig-like"
FT                   /evidence="ECO:0000259|Pfam:PF02927"
FT   DOMAIN          96..537
FT                   /note="Glycoside hydrolase family 9"
FT                   /evidence="ECO:0000259|Pfam:PF00759"
FT   ACT_SITE        515
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT   ACT_SITE        524
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ   SEQUENCE   543 AA;  59811 MW;  8E6BD71A3045C8B0 CRC64;
     MDKTRQAGIA INQLGYRSKD VKIAIRRDGS GGFSLVDAGS GETVWQGETG QAEWNEASGG
     MVSRGDFSGW QQPGVYRIET DKGEISYPFV IADDVYEEAH RALLKAFYFF RCGMELEERH
     AGPWTHRACH LTPAIVYGEE EKRVDAHGGW HDAGDYGKYV GPGAKAVADL LLAEEFYPQA
     FKRELGIPES GGFMPDALQE CRYELEWMLR MQDGATGGVY HKLTTLQFPP GDTMPEDDTA
     ELYVSPISAT ATGCFAAAMA MAARRYRPYD EAFAASCLRA AEKAWDWLEG HPGYPGFRNP
     PAVSTGEYGD EQDADERYWA AAELYRTTGE SKYHEAFRQL VDAVQPFGLF ELGWANMSGY
     GTIAYLFSER EQSPALAAQL REGLLRRADE LAAVCAADGY GISLQPEQYI WGSNMLVMNH
     AMLLLVANRL SGSAAYERHA IKHVHYLFGA NVLGMSYVTG LGTKPILYPH HRPSEGDGVD
     APVPGLVSGG PNYRLQDDYA REHLAGKAPA ASFADVMESY STNEVTIYWN SPAVFVLSHF
     VGL
//