ID   A0A3A5M754_9MICO        Unreviewed;       787 AA.
AC   A0A3A5M754;
DT   05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT   05-DEC-2018, sequence version 1.
DT   27-MAR-2024, entry version 21.
DE   SubName: Full=Glycogen debranching enzyme GlgX {ECO:0000313|EMBL:RJT84565.1};
GN   Name=glgX {ECO:0000313|EMBL:RJT84565.1};
GN   ORFNames=D6T64_20560 {ECO:0000313|EMBL:RJT84565.1};
OS   Cryobacterium melibiosiphilum.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC   Cryobacterium.
OX   NCBI_TaxID=995039 {ECO:0000313|EMBL:RJT84565.1, ECO:0000313|Proteomes:UP000272015};
RN   [1] {ECO:0000313|EMBL:RJT84565.1, ECO:0000313|Proteomes:UP000272015}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Hh39 {ECO:0000313|EMBL:RJT84565.1,
RC   ECO:0000313|Proteomes:UP000272015};
RA   Liu Q., Xin Y.-H.;
RT   "Novel species of Cryobacterium.";
RL   Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|ARBA:ARBA00008061}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RJT84565.1}.
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DR   EMBL; QZVS01000097; RJT84565.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A3A5M754; -.
DR   OrthoDB; 3236218at2; -.
DR   Proteomes; UP000272015; Unassembled WGS sequence.
DR   GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR   CDD; cd11326; AmyAc_Glg_debranch; 1.
DR   CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR011837; Glycogen_debranch_GlgX.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   NCBIfam; TIGR02100; glgX_debranch; 1.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF00128; Alpha-amylase; 2.
DR   Pfam; PF02922; CBM_48; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
FT   DOMAIN          132..566
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   REGION          464..492
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          733..787
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        472..487
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        755..777
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   787 AA;  87332 MW;  192D132B48F11656 CRC64;
     METWPGTAYP LGATYDGSGT NFALFSEAAE RVELCLFDDD GVETRVEVRE SAAFIWHCYL
     PLVQPGQRYG YRVHAEYAPG AGQRANSNKL LLDPYAKATC GTIDWDPSLF SYNFGDPESR
     NDDDSAPHMM LGVVVNPFFD WAGDRPLRTP YSESLIYEAH VKGLTQLQTE VPESQRGTYA
     GVAHPSVIDH LQKLGVTAIE LMPVHQFVNE GTLQDQGLNN YWGYNTIGFF APHNAYSSTG
     DLGQQVQEFK GMVRSMHDAG IEVILDVVYN HTAEGNHLGP TISFKGIDNE AYYRLMDDDK
     RYYMDYTGTG NTLNVRHPHV LQLIMDSLRY WVLEMHVDGF RFDLASALAR DLYDVDKLST
     FFELVQQDPI VSQVKLIAEP WDVGPGGYQV GNFPSQWSEW NGKYRDTVRD FWRGEPSTLG
     EFASRVAGSA DLYEHSGRRP MASINFITAH DGFTIADLVS YNEKHNDANG ENNQDGESNN
     RSWNSGAEGP TDDAAVRSLR ARQQRNFIAT LLLSQGVPML LHGDELGRTQ QGNNNTYAQD
     SELSWIHWDE ADVPLIEFTA AVSRLRKEHP TFRRSRFFDG HPGRRVEGAS LPDIVWLSPD
     GDEMKSEDWD ESLARTVAKF LNGQGIRERD SRGKNVTDVN FLLCFNADPE DVEFTLPPRQ
     YALAWEIVVD TAGEGADSIP RPAGAIHTVS ARSLVVLRAY QPPETLPDHS VAASLAGLSG
     PVVPLTPDIE LEPDLLGETT PAPEKSTDAV PAASPEEEGK PKSKPRSRAK AKPKTDPAKP
     QAEGAGE
//