ID   A0A3A5MXS6_9MICO        Unreviewed;       985 AA.
AC   A0A3A5MXS6;
DT   05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT   05-DEC-2018, sequence version 1.
DT   27-MAR-2024, entry version 21.
DE   RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE            EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
DE   Flags: Fragment;
GN   ORFNames=D6T64_05255 {ECO:0000313|EMBL:RJT89974.1};
OS   Cryobacterium melibiosiphilum.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC   Cryobacterium.
OX   NCBI_TaxID=995039 {ECO:0000313|EMBL:RJT89974.1, ECO:0000313|Proteomes:UP000272015};
RN   [1] {ECO:0000313|EMBL:RJT89974.1, ECO:0000313|Proteomes:UP000272015}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Hh39 {ECO:0000313|EMBL:RJT89974.1,
RC   ECO:0000313|Proteomes:UP000272015};
RA   Liu Q., Xin Y.-H.;
RT   "Novel species of Cryobacterium.";
RL   Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC         N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC         COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC         Evidence={ECO:0000256|ARBA:ARBA00001279};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RJT89974.1}.
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DR   EMBL; QZVS01000066; RJT89974.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A3A5MXS6; -.
DR   OrthoDB; 9776021at2; -.
DR   Proteomes; UP000272015; Unassembled WGS sequence.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR003356; DNA_methylase_A-5.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR041635; Type_ISP_LLaBIII_C.
DR   PANTHER; PTHR33841; DNA METHYLTRANSFERASE YEEA-RELATED; 1.
DR   PANTHER; PTHR33841:SF5; TYPE II METHYLTRANSFERASE M.HINDII; 1.
DR   Pfam; PF02384; N6_Mtase; 1.
DR   Pfam; PF18135; Type_ISP_C; 1.
DR   PRINTS; PR00507; N12N6MTFRASE.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS00092; N6_MTASE; 1.
PE   4: Predicted;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          234..420
FT                   /note="DNA methylase adenine-specific"
FT                   /evidence="ECO:0000259|Pfam:PF02384"
FT   DOMAIN          614..952
FT                   /note="Type ISP restriction-modification enzyme LLaBIII C-
FT                   terminal specificity"
FT                   /evidence="ECO:0000259|Pfam:PF18135"
FT   REGION          966..985
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   NON_TER         985
FT                   /evidence="ECO:0000313|EMBL:RJT89974.1"
SQ   SEQUENCE   985 AA;  108873 MW;  E195A9FAFB667D13 CRC64;
     MRGYIEVKAP GRKIDPDQMT GHDLEQWERQ RDLPNLIYTN GTRWRLYRDG EFIDEALLTE
     LELTEIGAGL RSNPKFEGIL RAFLAWKPDP IVSVNALVRA IAPLTRLLRG EVLDQLVGER
     KAVSAGAKEE DQPFIGLARD WRRLLFPQAD DKKFADGYAQ AVTFALLLAR TSGIEFEGRS
     LHDVGKDLGV QHSLMGRALQ LLIDDVAADF KVALDLLVTV IGSVRWERIR KTRRDVYLYL
     YESFLQEYDD ELRQASGTYY TPRELVTEMV RLTQDVLVQN VGIEEGFSSP GVFTIDPAMG
     TGTFLHSVVE TVRKHVEDEQ GPGAVPGAMT ELAGRLAGFE IQMGPYAVAE LRVAELFADA
     GATLPKDGLK LYVTDTLDDP TADETQIASG LHTIANSRKR ANAIKRNQKV NVIIGNPPYR
     ELAAGQGGWV EQGTPSQHGP STAILGDFLD GVPGRLAAKV KNLYVYFWRW ATWKVWESTP
     ADESGVVCFV TTSGYVTGTA FTGMRRHIRQ HASEGWIIDL TPEGHTPDVA TRMFPGVRQP
     LAIGIFVRRP GTSREVPADL HYISVRGRRA DKFTELQGLT LDAAEWRPVR KGWTDPFTAA
     ATSAWDEWPA VDQLMPWYSP GVFPTRTWVY SPSASCLEER WTQLVGEPEL ERRRVLMKDT
     TGAINVQFKD IPGHPHAARL NSIASLPVDN AMEKVVTIGF RAFDRQKLIA DPRLIHRARD
     GLWAAAAVPN QVYVVEQHSV SISDGPALLF SAHMPDFHHF NNRGGRTLPF LNPDGTPNFA
     PGLSTALTGK LNARVEAEDI LAYLAAVTAH PAFTQTFAVE LETPGIRIPI TADEVIWTKA
     VSIGRQVLWA QTFAQAFVDH PNGRPAHKLA YERGDSRRVS CLAPVSELPD GMVYDEEEQE
     IVLGNGRFGP VPRAVVEYEV GGRNVLRSWV AYRTKEPAGK RTSPLDDVNP GSWCCPSRYL
     TVAASSSVTM SGSGKAPPDD SGGAF
//