ID   A0A3A9GRD4_9FIRM        Unreviewed;       381 AA.
AC   A0A3A9GRD4;
DT   05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT   05-DEC-2018, sequence version 1.
DT   24-JAN-2024, entry version 17.
DE   SubName: Full=Class B sortase {ECO:0000313|EMBL:RKJ64532.1};
GN   ORFNames=D7Y06_11975 {ECO:0000313|EMBL:RKJ64532.1};
OS   Roseburia sp. 1XD42-69.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia.
OX   NCBI_TaxID=2320088 {ECO:0000313|EMBL:RKJ64532.1, ECO:0000313|Proteomes:UP000279120};
RN   [1] {ECO:0000313|EMBL:RKJ64532.1, ECO:0000313|Proteomes:UP000279120}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1XD42-69 {ECO:0000313|EMBL:RKJ64532.1,
RC   ECO:0000313|Proteomes:UP000279120};
RA   Liu C.;
RT   "Murine metabolic-syndrome-specific gut microbial biobank.";
RL   Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RKJ64532.1}.
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DR   EMBL; RAZG01000021; RKJ64532.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A3A9GRD4; -.
DR   OrthoDB; 9806013at2; -.
DR   Proteomes; UP000279120; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   CDD; cd05826; Sortase_B; 1.
DR   Gene3D; 2.40.260.10; Sortase; 1.
DR   InterPro; IPR005754; Sortase.
DR   InterPro; IPR023365; Sortase_dom-sf.
DR   InterPro; IPR009835; SrtB.
DR   Pfam; PF04203; Sortase; 1.
DR   SUPFAM; SSF63817; Sortase; 1.
PE   4: Predicted;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000279120};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        115..137
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   ACT_SITE        264
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR605754-1"
FT   ACT_SITE        364
FT                   /note="Acyl-thioester intermediate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR605754-1"
SQ   SEQUENCE   381 AA;  43963 MW;  1ED422192AADA6E3 CRC64;
     MEKYKIGRHE FDSKEEWENA LKDLKKIRDL VEQLDIEDAE DTLVLYQLLR NGDVTFDSEL
     GDAFFCDISD RVADNSMELI KQGSKKSFWP KVNVSSLIQR ETPGGPLEKE SIFKLAGVVC
     VVLALVCFGV YGLSVFAENR ATRKLEEVQS LKKENQVMGW FMERIDQEEP RQNSAQGDAS
     KLQMQEIDKP LEILPEYKEL HKKYPDLIGW VKIEGTSIDL PVMQTVDDFY LHNNIDGEED
     INGTLFLDSR DDVSHSMNLI VYGHNMRSGA MFGSLKQYLD DGFVAKHKQI QFDSIYEKGT
     YEVLYVCLTK VGYQDEPEYK YYNFIQPKSP EEFDAYFETI RACAVYDSKQ EVTEEDKFLT
     LSTCNSYIED GRLFVVAKKI Q
//